Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5824 and Pf1N1B4_5825 overlap by 4 nucleotides Pf1N1B4_5825 and Pf1N1B4_5826 overlap by 4 nucleotides Pf1N1B4_5826 and Pf1N1B4_5827 are separated by 8 nucleotides Pf1N1B4_5827 and Pf1N1B4_5828 are separated by 95 nucleotides
Pf1N1B4_5824 - Na(+) H(+) antiporter subunit D, at 259,307 to 260,983
_5824
Pf1N1B4_5825 - Na(+) H(+) antiporter subunit E, at 260,980 to 261,468
_5825
Pf1N1B4_5826 - Na(+) H(+) antiporter subunit F, at 261,465 to 261,743
_5826
Pf1N1B4_5827 - Na(+) H(+) antiporter subunit G, at 261,752 to 262,102
_5827
Pf1N1B4_5828 - Mobile element protein, at 262,198 to 263,499
_5828
Position (kb) 261
262
263 Strain fitness (log2 ratio) -2
-1
0
1 at 260.906 kb on + strand at 260.906 kb on + strand at 260.906 kb on + strand at 260.906 kb on + strand at 260.907 kb on - strand at 260.907 kb on - strand at 260.907 kb on - strand at 261.068 kb on + strand, within Pf1N1B4_5825 at 261.595 kb on - strand, within Pf1N1B4_5826 at 261.595 kb on - strand, within Pf1N1B4_5826 at 261.595 kb on - strand, within Pf1N1B4_5826 at 261.808 kb on + strand, within Pf1N1B4_5827 at 261.923 kb on + strand, within Pf1N1B4_5827 at 262.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 260,906 + -0.7 260,906 + -1.6 260,906 + -2.6 260,906 + -0.1 260,907 - +0.2 260,907 - -0.2 260,907 - -1.7 261,068 + Pf1N1B4_5825 0.18 -0.9 261,595 - Pf1N1B4_5826 0.47 -1.4 261,595 - Pf1N1B4_5826 0.47 -0.0 261,595 - Pf1N1B4_5826 0.47 -0.4 261,808 + Pf1N1B4_5827 0.16 -1.0 261,923 + Pf1N1B4_5827 0.49 -1.1 262,075 + -1.0
Or see this region's nucleotide sequence