Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5821 and Pf1N1B4_5822 are separated by 290 nucleotides Pf1N1B4_5822 and Pf1N1B4_5823 overlap by 1 nucleotides Pf1N1B4_5823 and Pf1N1B4_5824 are separated by 5 nucleotides
Pf1N1B4_5821 - Permease of the drug/metabolite transporter (DMT) superfamily, at 254,762 to 255,790
_5821
Pf1N1B4_5822 - Na(+) H(+) antiporter subunit A / Na(+) H(+) antiporter subunit B, at 256,081 to 258,957
_5822
Pf1N1B4_5823 - Na(+) H(+) antiporter subunit C, at 258,957 to 259,301
_5823
Pf1N1B4_5824 - Na(+) H(+) antiporter subunit D, at 259,307 to 260,983
_5824
Position (kb) 256
257
258
259 Strain fitness (log2 ratio) -2
-1
0
1
2 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.392 kb on + strand at 255.392 kb on + strand, within Pf1N1B4_5821 at 255.392 kb on + strand, within Pf1N1B4_5821 at 255.392 kb on + strand, within Pf1N1B4_5821 at 255.393 kb on - strand, within Pf1N1B4_5821 at 255.411 kb on + strand, within Pf1N1B4_5821 at 255.411 kb on + strand, within Pf1N1B4_5821 at 255.412 kb on - strand, within Pf1N1B4_5821 at 255.611 kb on + strand, within Pf1N1B4_5821 at 255.611 kb on + strand, within Pf1N1B4_5821 at 255.945 kb on - strand at 255.991 kb on + strand at 255.991 kb on + strand at 256.018 kb on + strand at 256.026 kb on + strand at 256.037 kb on + strand at 256.037 kb on + strand at 256.044 kb on - strand at 256.321 kb on + strand at 256.321 kb on + strand at 256.322 kb on - strand at 256.322 kb on - strand at 256.322 kb on - strand at 256.507 kb on + strand, within Pf1N1B4_5822 at 256.507 kb on + strand, within Pf1N1B4_5822 at 256.508 kb on - strand, within Pf1N1B4_5822 at 256.588 kb on + strand, within Pf1N1B4_5822 at 256.840 kb on + strand, within Pf1N1B4_5822 at 256.840 kb on + strand, within Pf1N1B4_5822 at 256.840 kb on + strand, within Pf1N1B4_5822 at 256.841 kb on - strand, within Pf1N1B4_5822 at 256.866 kb on + strand, within Pf1N1B4_5822 at 256.867 kb on - strand, within Pf1N1B4_5822 at 257.227 kb on - strand, within Pf1N1B4_5822 at 257.299 kb on + strand, within Pf1N1B4_5822 at 257.412 kb on + strand at 257.412 kb on + strand, within Pf1N1B4_5822 at 257.507 kb on - strand, within Pf1N1B4_5822 at 257.908 kb on + strand, within Pf1N1B4_5822 at 258.223 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.620 kb on - strand, within Pf1N1B4_5822 at 258.829 kb on + strand at 258.898 kb on - strand at 259.065 kb on + strand, within Pf1N1B4_5823 at 259.292 kb on + strand at 259.496 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.800 kb on - strand, within Pf1N1B4_5824 at 259.856 kb on + strand, within Pf1N1B4_5824
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 255,188 + Pf1N1B4_5821 0.41 -0.2 255,188 + Pf1N1B4_5821 0.41 -0.1 255,188 + Pf1N1B4_5821 0.41 +0.0 255,188 + Pf1N1B4_5821 0.41 +2.4 255,392 + +0.0 255,392 + Pf1N1B4_5821 0.61 +0.9 255,392 + Pf1N1B4_5821 0.61 -0.5 255,392 + Pf1N1B4_5821 0.61 -1.0 255,393 - Pf1N1B4_5821 0.61 -1.5 255,411 + Pf1N1B4_5821 0.63 +0.6 255,411 + Pf1N1B4_5821 0.63 +0.6 255,412 - Pf1N1B4_5821 0.63 -0.8 255,611 + Pf1N1B4_5821 0.83 +0.1 255,611 + Pf1N1B4_5821 0.83 +1.6 255,945 - +0.6 255,991 + +1.2 255,991 + +0.0 256,018 + +0.2 256,026 + +0.9 256,037 + +1.6 256,037 + -2.0 256,044 - -0.4 256,321 + -2.0 256,321 + -0.7 256,322 - +0.3 256,322 - -1.7 256,322 - -0.5 256,507 + Pf1N1B4_5822 0.15 +0.6 256,507 + Pf1N1B4_5822 0.15 -1.6 256,508 - Pf1N1B4_5822 0.15 -2.0 256,588 + Pf1N1B4_5822 0.18 -2.7 256,840 + Pf1N1B4_5822 0.26 -2.3 256,840 + Pf1N1B4_5822 0.26 -0.6 256,840 + Pf1N1B4_5822 0.26 +0.6 256,841 - Pf1N1B4_5822 0.26 -1.0 256,866 + Pf1N1B4_5822 0.27 -0.4 256,867 - Pf1N1B4_5822 0.27 -1.1 257,227 - Pf1N1B4_5822 0.40 -2.0 257,299 + Pf1N1B4_5822 0.42 -1.7 257,412 + -0.4 257,412 + Pf1N1B4_5822 0.46 -1.7 257,507 - Pf1N1B4_5822 0.50 +0.0 257,908 + Pf1N1B4_5822 0.64 -0.4 258,223 + Pf1N1B4_5822 0.74 -0.4 258,619 + Pf1N1B4_5822 0.88 -2.5 258,619 + Pf1N1B4_5822 0.88 -1.7 258,619 + Pf1N1B4_5822 0.88 -0.1 258,620 - Pf1N1B4_5822 0.88 -0.2 258,829 + -2.0 258,898 - -1.7 259,065 + Pf1N1B4_5823 0.31 -0.4 259,292 + -1.1 259,496 + Pf1N1B4_5824 0.11 -0.4 259,799 + Pf1N1B4_5824 0.29 +0.6 259,799 + Pf1N1B4_5824 0.29 -1.2 259,799 + Pf1N1B4_5824 0.29 -0.5 259,799 + +0.6 259,799 + Pf1N1B4_5824 0.29 +0.6 259,799 + Pf1N1B4_5824 0.29 -0.4 259,799 + Pf1N1B4_5824 0.29 -1.0 259,800 - Pf1N1B4_5824 0.29 -0.4 259,856 + Pf1N1B4_5824 0.33 +0.0
Or see this region's nucleotide sequence