Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5719 and Pf1N1B4_5720 are separated by 67 nucleotides Pf1N1B4_5720 and Pf1N1B4_5721 are separated by 205 nucleotides Pf1N1B4_5721 and Pf1N1B4_5722 overlap by 4 nucleotides Pf1N1B4_5722 and Pf1N1B4_5723 are separated by 0 nucleotides Pf1N1B4_5723 and Pf1N1B4_5724 are separated by 34 nucleotides Pf1N1B4_5724 and Pf1N1B4_5725 overlap by 16 nucleotides
Pf1N1B4_5719 - hypothetical protein, at 162,369 to 163,043
_5719
Pf1N1B4_5720 - hypothetical protein, at 163,111 to 163,356
_5720
Pf1N1B4_5721 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,562 to 163,714
_5721
Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091
_5722
Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424
_5723
Pf1N1B4_5724 - High-affinity iron permease, at 164,459 to 164,941
_5724
Pf1N1B4_5725 - High-affinity iron permease, at 164,926 to 165,300
_5725
Position (kb) 163
164
165 Strain fitness (log2 ratio) -2
-1
0
1
2 at 162.759 kb on + strand, within Pf1N1B4_5719 at 162.760 kb on - strand, within Pf1N1B4_5719 at 162.876 kb on - strand, within Pf1N1B4_5719 at 162.957 kb on + strand, within Pf1N1B4_5719 at 162.958 kb on - strand at 163.056 kb on + strand at 163.058 kb on + strand at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.177 kb on - strand, within Pf1N1B4_5720 at 163.394 kb on - strand at 163.394 kb on - strand at 163.394 kb on - strand at 163.472 kb on + strand at 163.537 kb on - strand at 163.558 kb on + strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.561 kb on + strand at 163.562 kb on - strand at 163.568 kb on - strand at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.917 kb on - strand, within Pf1N1B4_5722 at 163.936 kb on - strand, within Pf1N1B4_5722 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.801 kb on + strand, within Pf1N1B4_5724 at 164.802 kb on - strand, within Pf1N1B4_5724
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 162,759 + Pf1N1B4_5719 0.58 -0.6 162,760 - Pf1N1B4_5719 0.58 -0.1 162,876 - Pf1N1B4_5719 0.75 +0.6 162,957 + Pf1N1B4_5719 0.87 +0.6 162,958 - +2.2 163,056 + -0.4 163,058 + -0.4 163,174 - Pf1N1B4_5720 0.26 +0.8 163,174 - Pf1N1B4_5720 0.26 -0.7 163,176 + Pf1N1B4_5720 0.26 -1.7 163,176 + Pf1N1B4_5720 0.26 +1.2 163,177 - Pf1N1B4_5720 0.27 -1.0 163,394 - -1.2 163,394 - -1.3 163,394 - -1.0 163,472 + +1.6 163,537 - +0.6 163,558 + -0.0 163,559 - -0.3 163,559 - -0.2 163,559 - +0.6 163,561 + -0.3 163,562 - -0.3 163,568 - +0.3 163,679 + Pf1N1B4_5721 0.76 -0.0 163,679 + Pf1N1B4_5721 0.76 -0.6 163,680 - Pf1N1B4_5721 0.77 +0.2 163,680 - Pf1N1B4_5721 0.77 +1.0 163,916 + Pf1N1B4_5722 0.54 -1.0 163,916 + Pf1N1B4_5722 0.54 -1.0 163,916 + Pf1N1B4_5722 0.54 +0.6 163,917 - Pf1N1B4_5722 0.54 -0.4 163,936 - Pf1N1B4_5722 0.59 -0.2 164,798 + Pf1N1B4_5724 0.70 -1.7 164,798 + Pf1N1B4_5724 0.70 +0.9 164,798 + Pf1N1B4_5724 0.70 -2.7 164,801 + Pf1N1B4_5724 0.71 +1.6 164,802 - Pf1N1B4_5724 0.71 +0.6
Or see this region's nucleotide sequence