Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5575 and Pf1N1B4_5576 are separated by 838 nucleotides Pf1N1B4_5576 and Pf1N1B4_5577 are separated by 217 nucleotides
Pf1N1B4_5575 - hypothetical protein, at 345,254 to 345,634
_5575
Pf1N1B4_5576 - hypothetical protein, at 346,473 to 346,823
_5576
Pf1N1B4_5577 - twitching motility protein PilG, at 347,041 to 347,319
_5577
Position (kb) 346
347 Strain fitness (log2 ratio) -2
-1
0
1
2 at 345.536 kb on + strand, within Pf1N1B4_5575 at 345.536 kb on + strand, within Pf1N1B4_5575 at 345.537 kb on - strand, within Pf1N1B4_5575 at 345.554 kb on - strand, within Pf1N1B4_5575 at 345.679 kb on + strand at 345.680 kb on - strand at 345.680 kb on - strand at 345.680 kb on - strand at 345.699 kb on - strand at 345.805 kb on - strand at 345.875 kb on + strand at 345.876 kb on - strand at 345.887 kb on + strand at 345.888 kb on - strand at 345.888 kb on - strand at 345.952 kb on + strand at 345.952 kb on + strand at 345.952 kb on + strand at 345.952 kb on + strand at 345.952 kb on + strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.953 kb on - strand at 345.956 kb on + strand at 345.956 kb on + strand at 345.956 kb on + strand at 345.956 kb on + strand at 345.957 kb on - strand at 345.957 kb on - strand at 345.963 kb on + strand at 345.963 kb on + strand at 345.963 kb on + strand at 345.964 kb on - strand at 345.964 kb on - strand at 346.013 kb on + strand at 346.013 kb on + strand at 346.013 kb on + strand at 346.019 kb on + strand at 346.019 kb on + strand at 346.019 kb on + strand at 346.020 kb on - strand at 346.020 kb on - strand at 346.020 kb on - strand at 346.020 kb on - strand at 346.021 kb on + strand at 346.022 kb on - strand at 346.058 kb on + strand at 346.059 kb on - strand at 346.059 kb on - strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.060 kb on + strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.061 kb on - strand at 346.162 kb on + strand at 346.162 kb on + strand at 346.163 kb on - strand at 346.171 kb on + strand at 346.171 kb on + strand at 346.171 kb on + strand at 346.172 kb on - strand at 346.202 kb on + strand at 346.231 kb on + strand at 346.257 kb on - strand at 346.351 kb on - strand at 346.351 kb on - strand at 346.664 kb on + strand, within Pf1N1B4_5576 at 346.670 kb on + strand, within Pf1N1B4_5576 at 346.670 kb on + strand, within Pf1N1B4_5576 at 346.671 kb on - strand at 346.767 kb on + strand, within Pf1N1B4_5576 at 346.768 kb on - strand, within Pf1N1B4_5576 at 346.768 kb on - strand, within Pf1N1B4_5576 at 346.768 kb on - strand, within Pf1N1B4_5576 at 346.768 kb on - strand, within Pf1N1B4_5576 at 346.771 kb on - strand, within Pf1N1B4_5576 at 347.051 kb on + strand at 347.052 kb on - strand at 347.052 kb on - strand at 347.102 kb on + strand, within Pf1N1B4_5577 at 347.102 kb on + strand, within Pf1N1B4_5577 at 347.157 kb on + strand, within Pf1N1B4_5577 at 347.157 kb on + strand, within Pf1N1B4_5577 at 347.179 kb on - strand, within Pf1N1B4_5577 at 347.319 kb on + strand at 347.320 kb on - strand at 347.320 kb on - strand at 347.391 kb on + strand at 347.392 kb on - strand at 347.392 kb on - strand at 347.424 kb on + strand at 347.480 kb on + strand at 347.480 kb on + strand at 347.481 kb on - strand at 347.505 kb on + strand at 347.506 kb on - strand at 347.516 kb on - strand at 347.519 kb on + strand at 347.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 345,536 + Pf1N1B4_5575 0.74 +0.4 345,536 + Pf1N1B4_5575 0.74 +0.0 345,537 - Pf1N1B4_5575 0.74 +1.8 345,554 - Pf1N1B4_5575 0.79 +0.8 345,679 + -0.3 345,680 - -2.4 345,680 - +0.3 345,680 - +0.4 345,699 - -0.3 345,805 - +0.3 345,875 + +1.0 345,876 - -0.4 345,887 + -0.3 345,888 - +0.2 345,888 - -0.6 345,952 + -0.6 345,952 + +1.2 345,952 + -1.7 345,952 + +0.2 345,952 + +0.5 345,953 - +0.3 345,953 - -0.6 345,953 - -0.7 345,953 - -0.4 345,953 - -1.0 345,953 - +0.0 345,953 - -0.2 345,953 - +1.6 345,956 + +0.4 345,956 + -0.4 345,956 + +1.0 345,956 + +1.0 345,957 - -0.0 345,957 - +0.7 345,963 + +0.4 345,963 + +0.0 345,963 + -1.0 345,964 - +1.3 345,964 - -1.3 346,013 + -0.5 346,013 + +1.2 346,013 + -0.3 346,019 + +1.0 346,019 + -0.2 346,019 + -0.1 346,020 - +1.4 346,020 - +1.9 346,020 - +0.0 346,020 - +0.5 346,021 + -1.6 346,022 - +0.3 346,058 + +1.6 346,059 - -1.0 346,059 - -0.3 346,060 + +0.2 346,060 + +0.0 346,060 + -0.7 346,060 + -1.1 346,060 + -1.0 346,060 + +0.2 346,061 - +0.8 346,061 - -1.6 346,061 - -1.0 346,061 - -1.3 346,061 - +0.0 346,061 - -0.8 346,061 - -0.6 346,061 - -2.0 346,061 - -1.1 346,061 - +0.3 346,061 - +0.0 346,061 - +1.8 346,061 - -0.3 346,162 + -0.6 346,162 + -2.6 346,163 - -0.4 346,171 + -1.0 346,171 + +0.4 346,171 + +0.0 346,172 - -0.4 346,202 + -0.4 346,231 + -0.1 346,257 - +0.8 346,351 - +0.0 346,351 - +0.0 346,664 + Pf1N1B4_5576 0.54 -1.8 346,670 + Pf1N1B4_5576 0.56 +0.6 346,670 + Pf1N1B4_5576 0.56 -0.2 346,671 - +1.0 346,767 + Pf1N1B4_5576 0.84 +0.0 346,768 - Pf1N1B4_5576 0.84 -2.6 346,768 - Pf1N1B4_5576 0.84 -0.6 346,768 - Pf1N1B4_5576 0.84 -0.4 346,768 - Pf1N1B4_5576 0.84 -1.3 346,771 - Pf1N1B4_5576 0.85 -1.6 347,051 + +1.0 347,052 - +0.7 347,052 - -0.1 347,102 + Pf1N1B4_5577 0.22 +0.3 347,102 + Pf1N1B4_5577 0.22 -1.0 347,157 + Pf1N1B4_5577 0.42 +0.3 347,157 + Pf1N1B4_5577 0.42 -1.4 347,179 - Pf1N1B4_5577 0.49 -1.8 347,319 + +0.3 347,320 - +0.2 347,320 - -0.7 347,391 + +0.7 347,392 - +0.0 347,392 - +0.7 347,424 + -0.3 347,480 + -0.6 347,480 + +0.3 347,481 - +0.4 347,505 + -0.4 347,506 - -1.0 347,516 - -1.2 347,519 + +0.1 347,520 - -0.2
Or see this region's nucleotide sequence