Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5392 and Pf1N1B4_5393 are separated by 2 nucleotides Pf1N1B4_5393 and Pf1N1B4_5394 overlap by 46 nucleotides Pf1N1B4_5394 and Pf1N1B4_5395 are separated by 523 nucleotides
Pf1N1B4_5392 - hypothetical protein, at 123,107 to 124,111
_5392
Pf1N1B4_5393 - hypothetical protein, at 124,114 to 125,103
_5393
Pf1N1B4_5394 - hypothetical protein, at 125,058 to 125,429
_5394
Pf1N1B4_5395 - hypothetical protein, at 125,953 to 127,242
_5395
Position (kb)
124
125
126 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 123.151 kb on + strand at 123.418 kb on - strand, within Pf1N1B4_5392 at 123.418 kb on - strand, within Pf1N1B4_5392 at 123.418 kb on - strand, within Pf1N1B4_5392 at 123.424 kb on + strand, within Pf1N1B4_5392 at 123.425 kb on - strand, within Pf1N1B4_5392 at 123.425 kb on - strand, within Pf1N1B4_5392 at 123.491 kb on + strand, within Pf1N1B4_5392 at 123.492 kb on - strand at 123.506 kb on - strand, within Pf1N1B4_5392 at 123.535 kb on + strand, within Pf1N1B4_5392 at 123.536 kb on - strand, within Pf1N1B4_5392 at 123.536 kb on - strand, within Pf1N1B4_5392 at 123.806 kb on - strand, within Pf1N1B4_5392 at 123.810 kb on - strand, within Pf1N1B4_5392 at 123.896 kb on + strand, within Pf1N1B4_5392 at 123.897 kb on - strand, within Pf1N1B4_5392 at 123.897 kb on - strand, within Pf1N1B4_5392 at 123.985 kb on - strand, within Pf1N1B4_5392 at 123.988 kb on + strand at 123.988 kb on + strand, within Pf1N1B4_5392 at 123.988 kb on + strand, within Pf1N1B4_5392 at 123.988 kb on + strand at 123.989 kb on - strand, within Pf1N1B4_5392 at 123.991 kb on + strand, within Pf1N1B4_5392 at 123.991 kb on + strand, within Pf1N1B4_5392 at 123.991 kb on + strand at 123.991 kb on + strand, within Pf1N1B4_5392 at 123.991 kb on + strand at 123.996 kb on - strand, within Pf1N1B4_5392 at 124.268 kb on + strand, within Pf1N1B4_5393 at 124.288 kb on + strand, within Pf1N1B4_5393 at 124.289 kb on - strand, within Pf1N1B4_5393 at 124.555 kb on - strand, within Pf1N1B4_5393 at 124.922 kb on - strand, within Pf1N1B4_5393 at 125.022 kb on + strand at 125.023 kb on - strand at 125.023 kb on - strand at 125.283 kb on + strand, within Pf1N1B4_5394 at 125.460 kb on + strand at 125.506 kb on + strand at 125.506 kb on + strand at 125.506 kb on + strand at 125.506 kb on + strand at 125.506 kb on + strand at 125.506 kb on + strand at 125.506 kb on + strand at 125.506 kb on + strand at 125.507 kb on - strand at 125.594 kb on - strand at 125.753 kb on + strand at 125.753 kb on + strand at 125.773 kb on + strand at 125.773 kb on + strand at 125.773 kb on + strand at 125.774 kb on - strand at 125.774 kb on - strand at 125.955 kb on - strand at 125.955 kb on - strand at 125.955 kb on - strand at 125.985 kb on + strand at 125.987 kb on - strand at 125.987 kb on - strand at 126.011 kb on + strand at 126.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 123,151 + -0.6 123,418 - Pf1N1B4_5392 0.31 -1.6 123,418 - Pf1N1B4_5392 0.31 +0.1 123,418 - Pf1N1B4_5392 0.31 +0.6 123,424 + Pf1N1B4_5392 0.32 +0.3 123,425 - Pf1N1B4_5392 0.32 -1.0 123,425 - Pf1N1B4_5392 0.32 +0.4 123,491 + Pf1N1B4_5392 0.38 +0.3 123,492 - -0.6 123,506 - Pf1N1B4_5392 0.40 +1.8 123,535 + Pf1N1B4_5392 0.43 -0.2 123,536 - Pf1N1B4_5392 0.43 -0.8 123,536 - Pf1N1B4_5392 0.43 -0.3 123,806 - Pf1N1B4_5392 0.70 +0.2 123,810 - Pf1N1B4_5392 0.70 +0.2 123,896 + Pf1N1B4_5392 0.79 -0.2 123,897 - Pf1N1B4_5392 0.79 -1.8 123,897 - Pf1N1B4_5392 0.79 +0.6 123,985 - Pf1N1B4_5392 0.87 +0.0 123,988 + +0.4 123,988 + Pf1N1B4_5392 0.88 +2.6 123,988 + Pf1N1B4_5392 0.88 -0.2 123,988 + +3.3 123,989 - Pf1N1B4_5392 0.88 -1.0 123,991 + Pf1N1B4_5392 0.88 +0.3 123,991 + Pf1N1B4_5392 0.88 -0.3 123,991 + +1.8 123,991 + Pf1N1B4_5392 0.88 -1.0 123,991 + +1.0 123,996 - Pf1N1B4_5392 0.88 +1.0 124,268 + Pf1N1B4_5393 0.16 +1.4 124,288 + Pf1N1B4_5393 0.18 -1.6 124,289 - Pf1N1B4_5393 0.18 -1.8 124,555 - Pf1N1B4_5393 0.45 -1.7 124,922 - Pf1N1B4_5393 0.82 -0.4 125,022 + +1.0 125,023 - -1.0 125,023 - -2.4 125,283 + Pf1N1B4_5394 0.60 +1.3 125,460 + -1.0 125,506 + +0.5 125,506 + +1.0 125,506 + +0.0 125,506 + +1.0 125,506 + -0.4 125,506 + -2.0 125,506 + +0.0 125,506 + +0.4 125,507 - -1.8 125,594 - -2.0 125,753 + +0.2 125,753 + +0.2 125,773 + +1.6 125,773 + +1.0 125,773 + -1.0 125,774 - -2.3 125,774 - +1.0 125,955 - -0.3 125,955 - -0.2 125,955 - +0.1 125,985 + +1.0 125,987 - +0.0 125,987 - +0.6 126,011 + +1.3 126,012 - +0.4
Or see this region's nucleotide sequence