Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5363 and Pf1N1B4_5364 are separated by 184 nucleotides Pf1N1B4_5364 and Pf1N1B4_5365 are separated by 64 nucleotides Pf1N1B4_5365 and Pf1N1B4_5366 are separated by 145 nucleotides Pf1N1B4_5366 and Pf1N1B4_5367 are separated by 291 nucleotides
Pf1N1B4_5363 - hypothetical protein, at 97,638 to 98,639
_5363
Pf1N1B4_5364 - hypothetical protein, at 98,824 to 98,964
_5364
Pf1N1B4_5365 - hypothetical protein, at 99,029 to 99,457
_5365
Pf1N1B4_5366 - hypothetical protein, at 99,603 to 99,803
_5366
Pf1N1B4_5367 - hypothetical protein, at 100,095 to 100,583
_5367
Position (kb) 99
100 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 98.038 kb on + strand, within Pf1N1B4_5363 at 98.038 kb on + strand, within Pf1N1B4_5363 at 98.039 kb on - strand, within Pf1N1B4_5363 at 98.185 kb on + strand, within Pf1N1B4_5363 at 98.230 kb on + strand, within Pf1N1B4_5363 at 98.240 kb on + strand, within Pf1N1B4_5363 at 98.266 kb on + strand, within Pf1N1B4_5363 at 98.266 kb on + strand at 98.335 kb on + strand, within Pf1N1B4_5363 at 98.479 kb on + strand, within Pf1N1B4_5363 at 98.479 kb on + strand, within Pf1N1B4_5363 at 98.480 kb on - strand, within Pf1N1B4_5363 at 98.482 kb on + strand, within Pf1N1B4_5363 at 98.483 kb on - strand, within Pf1N1B4_5363 at 98.557 kb on + strand at 98.656 kb on + strand at 98.657 kb on - strand at 98.657 kb on - strand at 98.661 kb on + strand at 98.662 kb on - strand at 98.662 kb on - strand at 98.678 kb on + strand at 98.678 kb on + strand at 98.678 kb on + strand at 98.679 kb on - strand at 98.692 kb on + strand at 98.774 kb on - strand at 98.797 kb on + strand at 98.797 kb on + strand at 98.797 kb on + strand at 98.823 kb on + strand at 98.824 kb on - strand at 98.932 kb on - strand at 98.978 kb on + strand at 98.979 kb on - strand at 98.979 kb on - strand at 99.090 kb on + strand, within Pf1N1B4_5365 at 99.090 kb on + strand, within Pf1N1B4_5365 at 99.090 kb on + strand, within Pf1N1B4_5365 at 99.090 kb on + strand, within Pf1N1B4_5365 at 99.091 kb on - strand, within Pf1N1B4_5365 at 99.160 kb on + strand, within Pf1N1B4_5365 at 99.160 kb on + strand, within Pf1N1B4_5365 at 99.160 kb on + strand at 99.161 kb on - strand, within Pf1N1B4_5365 at 99.482 kb on + strand at 99.482 kb on + strand at 99.483 kb on - strand at 99.583 kb on + strand at 99.583 kb on + strand at 99.584 kb on - strand at 99.584 kb on - strand at 99.746 kb on + strand, within Pf1N1B4_5366 at 99.895 kb on + strand at 99.896 kb on - strand at 99.896 kb on - strand at 99.896 kb on - strand at 99.896 kb on - strand at 100.062 kb on + strand at 100.077 kb on + strand at 100.077 kb on + strand at 100.077 kb on + strand at 100.174 kb on + strand, within Pf1N1B4_5367 at 100.249 kb on + strand, within Pf1N1B4_5367 at 100.273 kb on + strand, within Pf1N1B4_5367 at 100.274 kb on - strand, within Pf1N1B4_5367 at 100.274 kb on - strand, within Pf1N1B4_5367 at 100.274 kb on - strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.313 kb on + strand, within Pf1N1B4_5367 at 100.314 kb on - strand, within Pf1N1B4_5367
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 98,038 + Pf1N1B4_5363 0.40 -0.8 98,038 + Pf1N1B4_5363 0.40 +0.6 98,039 - Pf1N1B4_5363 0.40 -0.4 98,185 + Pf1N1B4_5363 0.55 -1.0 98,230 + Pf1N1B4_5363 0.59 +0.2 98,240 + Pf1N1B4_5363 0.60 -1.3 98,266 + Pf1N1B4_5363 0.63 +0.7 98,266 + +0.0 98,335 + Pf1N1B4_5363 0.70 +0.0 98,479 + Pf1N1B4_5363 0.84 +0.7 98,479 + Pf1N1B4_5363 0.84 -0.4 98,480 - Pf1N1B4_5363 0.84 -1.2 98,482 + Pf1N1B4_5363 0.84 -0.8 98,483 - Pf1N1B4_5363 0.84 -0.2 98,557 + +1.0 98,656 + +0.0 98,657 - -2.4 98,657 - -2.0 98,661 + -0.2 98,662 - -1.6 98,662 - -0.6 98,678 + +2.0 98,678 + +0.0 98,678 + -0.6 98,679 - -0.2 98,692 + -1.8 98,774 - +1.0 98,797 + +0.3 98,797 + -0.3 98,797 + +0.3 98,823 + +1.4 98,824 - +0.0 98,932 - +2.0 98,978 + +3.1 98,979 - -0.2 98,979 - -0.6 99,090 + Pf1N1B4_5365 0.14 +1.4 99,090 + Pf1N1B4_5365 0.14 +1.2 99,090 + Pf1N1B4_5365 0.14 +0.7 99,090 + Pf1N1B4_5365 0.14 -0.3 99,091 - Pf1N1B4_5365 0.14 +0.4 99,160 + Pf1N1B4_5365 0.31 +0.7 99,160 + Pf1N1B4_5365 0.31 -0.3 99,160 + +1.0 99,161 - Pf1N1B4_5365 0.31 +0.7 99,482 + +0.4 99,482 + +0.2 99,483 - +2.0 99,583 + -0.6 99,583 + +0.2 99,584 - +0.5 99,584 - -0.4 99,746 + Pf1N1B4_5366 0.71 -2.4 99,895 + +0.0 99,896 - -0.3 99,896 - -0.4 99,896 - +0.1 99,896 - +0.0 100,062 + +0.8 100,077 + +0.0 100,077 + +1.0 100,077 + -0.9 100,174 + Pf1N1B4_5367 0.16 +0.6 100,249 + Pf1N1B4_5367 0.31 -2.6 100,273 + Pf1N1B4_5367 0.36 -0.3 100,274 - Pf1N1B4_5367 0.37 +0.7 100,274 - Pf1N1B4_5367 0.37 +0.4 100,274 - Pf1N1B4_5367 0.37 -1.6 100,313 + Pf1N1B4_5367 0.45 +1.3 100,313 + Pf1N1B4_5367 0.45 -0.4 100,313 + Pf1N1B4_5367 0.45 +0.3 100,313 + Pf1N1B4_5367 0.45 +0.6 100,313 + Pf1N1B4_5367 0.45 +1.0 100,314 - Pf1N1B4_5367 0.45 -0.3
Or see this region's nucleotide sequence