Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5329 and Pf1N1B4_5330 are separated by 25 nucleotides Pf1N1B4_5330 and Pf1N1B4_5331 are separated by 356 nucleotides Pf1N1B4_5331 and Pf1N1B4_5332 are separated by 2 nucleotides Pf1N1B4_5332 and Pf1N1B4_5333 are separated by 11 nucleotides Pf1N1B4_5333 and Pf1N1B4_5334 are separated by 131 nucleotides
Pf1N1B4_5329 - hypothetical protein, at 77,354 to 77,896
_5329
Pf1N1B4_5330 - hypothetical protein, at 77,922 to 78,233
_5330
Pf1N1B4_5331 - hypothetical protein, at 78,590 to 78,760
_5331
Pf1N1B4_5332 - hypothetical protein, at 78,763 to 78,957
_5332
Pf1N1B4_5333 - hypothetical protein, at 78,969 to 79,595
_5333
Pf1N1B4_5334 - hypothetical protein, at 79,727 to 79,930
_5334
Position (kb) 78
79 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 77.781 kb on + strand, within Pf1N1B4_5329 at 77.781 kb on + strand at 77.782 kb on - strand, within Pf1N1B4_5329 at 77.847 kb on + strand at 77.923 kb on + strand at 77.923 kb on + strand at 77.980 kb on + strand, within Pf1N1B4_5330 at 78.022 kb on - strand, within Pf1N1B4_5330 at 78.152 kb on + strand at 78.152 kb on + strand, within Pf1N1B4_5330 at 78.153 kb on - strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.314 kb on - strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.352 kb on - strand at 78.352 kb on - strand at 78.392 kb on + strand at 78.392 kb on + strand at 78.425 kb on + strand at 78.425 kb on + strand at 78.426 kb on - strand at 78.426 kb on - strand at 78.474 kb on + strand at 78.474 kb on + strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.729 kb on + strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.739 kb on - strand, within Pf1N1B4_5331 at 78.971 kb on - strand at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.106 kb on + strand, within Pf1N1B4_5333 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.446 kb on - strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.580 kb on + strand at 79.580 kb on + strand at 79.726 kb on - strand at 79.728 kb on + strand at 79.793 kb on + strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.797 kb on + strand, within Pf1N1B4_5334
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 77,781 + Pf1N1B4_5329 0.79 +1.2 77,781 + +0.0 77,782 - Pf1N1B4_5329 0.79 -0.9 77,847 + -1.4 77,923 + -1.2 77,923 + -1.1 77,980 + Pf1N1B4_5330 0.19 -0.9 78,022 - Pf1N1B4_5330 0.32 -2.0 78,152 + +0.0 78,152 + Pf1N1B4_5330 0.74 -1.6 78,153 - +3.0 78,313 + +0.6 78,313 + -1.3 78,313 + -1.3 78,313 + -0.1 78,314 - -1.6 78,336 + +1.0 78,336 + +0.2 78,336 + +1.0 78,336 + +0.0 78,351 + +1.6 78,351 + +0.0 78,351 + -0.5 78,352 - +0.2 78,352 - +0.6 78,392 + -0.4 78,392 + -1.0 78,425 + -1.3 78,425 + -0.8 78,426 - -1.8 78,426 - -0.3 78,474 + +2.6 78,474 + -1.0 78,475 - -2.3 78,475 - -2.6 78,475 - +1.0 78,475 - +1.3 78,475 - +1.0 78,597 - +1.5 78,597 - +0.0 78,597 - -0.3 78,729 + Pf1N1B4_5331 0.81 -0.6 78,730 - Pf1N1B4_5331 0.82 -0.5 78,730 - Pf1N1B4_5331 0.82 -0.2 78,730 - Pf1N1B4_5331 0.82 -2.3 78,739 - Pf1N1B4_5331 0.87 +0.5 78,971 - -0.6 79,045 + Pf1N1B4_5333 0.12 -0.4 79,045 + Pf1N1B4_5333 0.12 +0.6 79,046 - Pf1N1B4_5333 0.12 -0.6 79,046 - Pf1N1B4_5333 0.12 -1.2 79,046 - Pf1N1B4_5333 0.12 +0.7 79,106 + Pf1N1B4_5333 0.22 -1.3 79,268 + Pf1N1B4_5333 0.48 +2.0 79,268 + Pf1N1B4_5333 0.48 +0.3 79,269 - Pf1N1B4_5333 0.48 -0.7 79,269 - Pf1N1B4_5333 0.48 +1.0 79,393 - Pf1N1B4_5333 0.68 +1.8 79,393 - Pf1N1B4_5333 0.68 -0.4 79,446 - Pf1N1B4_5333 0.76 +1.0 79,501 + Pf1N1B4_5333 0.85 -1.8 79,501 + Pf1N1B4_5333 0.85 -0.5 79,502 - Pf1N1B4_5333 0.85 +0.1 79,502 - Pf1N1B4_5333 0.85 -0.2 79,576 + -2.2 79,576 + -2.0 79,576 + +1.4 79,576 + +1.0 79,577 - +1.4 79,577 - +0.3 79,577 - -1.8 79,577 - +0.1 79,577 - +1.4 79,580 + +0.6 79,580 + +1.0 79,726 - +2.0 79,728 + +1.4 79,793 + Pf1N1B4_5334 0.32 +0.7 79,794 - Pf1N1B4_5334 0.33 +0.3 79,794 - Pf1N1B4_5334 0.33 +0.3 79,794 - Pf1N1B4_5334 0.33 +0.0 79,794 - Pf1N1B4_5334 0.33 -0.5 79,797 + Pf1N1B4_5334 0.34 -1.2
Or see this region's nucleotide sequence