Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_532 and Pf1N1B4_533 are separated by 104 nucleotides Pf1N1B4_533 and Pf1N1B4_534 overlap by 46 nucleotides
Pf1N1B4_532 - Transcriptional regulators, LysR family, at 630,636 to 631,529
_532
Pf1N1B4_533 - Possible MFS Superfamily transporter precursor, at 631,634 to 632,824
_533
Pf1N1B4_534 - hypothetical protein, at 632,779 to 632,955
_534
Position (kb) 631
632
633 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 630.674 kb on - strand at 631.186 kb on + strand, within Pf1N1B4_532 at 631.240 kb on + strand at 631.240 kb on + strand at 631.240 kb on + strand, within Pf1N1B4_532 at 631.240 kb on + strand, within Pf1N1B4_532 at 631.240 kb on + strand, within Pf1N1B4_532 at 631.241 kb on - strand, within Pf1N1B4_532 at 631.241 kb on - strand, within Pf1N1B4_532 at 631.241 kb on - strand, within Pf1N1B4_532 at 631.241 kb on - strand, within Pf1N1B4_532 at 631.241 kb on - strand, within Pf1N1B4_532 at 631.241 kb on - strand at 631.241 kb on - strand, within Pf1N1B4_532 at 631.331 kb on - strand, within Pf1N1B4_532 at 631.793 kb on + strand at 631.793 kb on + strand, within Pf1N1B4_533 at 632.132 kb on + strand, within Pf1N1B4_533 at 632.132 kb on + strand at 632.132 kb on + strand, within Pf1N1B4_533 at 632.132 kb on + strand, within Pf1N1B4_533 at 632.132 kb on + strand, within Pf1N1B4_533 at 632.132 kb on + strand at 632.132 kb on + strand, within Pf1N1B4_533 at 632.133 kb on - strand, within Pf1N1B4_533 at 632.133 kb on - strand, within Pf1N1B4_533 at 632.133 kb on - strand, within Pf1N1B4_533 at 632.256 kb on + strand, within Pf1N1B4_533 at 632.261 kb on + strand, within Pf1N1B4_533 at 632.261 kb on + strand, within Pf1N1B4_533 at 632.261 kb on + strand, within Pf1N1B4_533 at 632.261 kb on + strand, within Pf1N1B4_533 at 632.261 kb on + strand, within Pf1N1B4_533 at 632.261 kb on + strand, within Pf1N1B4_533 at 632.262 kb on - strand, within Pf1N1B4_533 at 632.262 kb on - strand, within Pf1N1B4_533 at 632.262 kb on - strand, within Pf1N1B4_533 at 632.270 kb on + strand, within Pf1N1B4_533 at 632.271 kb on - strand, within Pf1N1B4_533 at 632.282 kb on + strand, within Pf1N1B4_533 at 632.450 kb on - strand, within Pf1N1B4_533 at 632.983 kb on + strand at 632.983 kb on + strand at 632.984 kb on - strand at 633.015 kb on + strand at 633.115 kb on + strand at 633.115 kb on + strand at 633.116 kb on - strand at 633.322 kb on - strand at 633.322 kb on - strand at 633.378 kb on - strand at 633.378 kb on - strand at 633.380 kb on - strand at 633.380 kb on - strand at 633.531 kb on + strand at 633.531 kb on + strand at 633.532 kb on - strand at 633.532 kb on - strand at 633.548 kb on + strand at 633.707 kb on - strand at 633.735 kb on + strand at 633.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 630,674 - +0.5 631,186 + Pf1N1B4_532 0.62 +3.7 631,240 + -0.1 631,240 + -0.7 631,240 + Pf1N1B4_532 0.68 -1.1 631,240 + Pf1N1B4_532 0.68 +0.2 631,240 + Pf1N1B4_532 0.68 -0.3 631,241 - Pf1N1B4_532 0.68 -1.9 631,241 - Pf1N1B4_532 0.68 +1.6 631,241 - Pf1N1B4_532 0.68 +0.6 631,241 - Pf1N1B4_532 0.68 +0.1 631,241 - Pf1N1B4_532 0.68 +0.9 631,241 - +0.9 631,241 - Pf1N1B4_532 0.68 -1.0 631,331 - Pf1N1B4_532 0.78 +0.7 631,793 + -0.1 631,793 + Pf1N1B4_533 0.13 -0.9 632,132 + Pf1N1B4_533 0.42 -0.7 632,132 + +0.9 632,132 + Pf1N1B4_533 0.42 +0.3 632,132 + Pf1N1B4_533 0.42 -0.4 632,132 + Pf1N1B4_533 0.42 -1.0 632,132 + +0.9 632,132 + Pf1N1B4_533 0.42 -0.9 632,133 - Pf1N1B4_533 0.42 -2.3 632,133 - Pf1N1B4_533 0.42 -0.7 632,133 - Pf1N1B4_533 0.42 +0.3 632,256 + Pf1N1B4_533 0.52 -1.1 632,261 + Pf1N1B4_533 0.53 -1.1 632,261 + Pf1N1B4_533 0.53 -0.3 632,261 + Pf1N1B4_533 0.53 +0.3 632,261 + Pf1N1B4_533 0.53 -0.1 632,261 + Pf1N1B4_533 0.53 +0.3 632,261 + Pf1N1B4_533 0.53 +0.2 632,262 - Pf1N1B4_533 0.53 -1.4 632,262 - Pf1N1B4_533 0.53 +1.6 632,262 - Pf1N1B4_533 0.53 -0.7 632,270 + Pf1N1B4_533 0.53 -1.1 632,271 - Pf1N1B4_533 0.53 +0.9 632,282 + Pf1N1B4_533 0.54 -1.1 632,450 - Pf1N1B4_533 0.69 -0.9 632,983 + -0.9 632,983 + +0.0 632,984 - -1.7 633,015 + +0.1 633,115 + +0.2 633,115 + -1.3 633,116 - +0.3 633,322 - +0.9 633,322 - -0.1 633,378 - -1.3 633,378 - -0.3 633,380 - -0.4 633,380 - -0.3 633,531 + +0.0 633,531 + -0.1 633,532 - +0.4 633,532 - +1.5 633,548 + -0.9 633,707 - -1.1 633,735 + +0.1 633,787 - +0.3
Or see this region's nucleotide sequence