Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5310 and Pf1N1B4_5311 are separated by 924 nucleotides Pf1N1B4_5311 and Pf1N1B4_5312 are separated by 242 nucleotides Pf1N1B4_5312 and Pf1N1B4_5313 are separated by 40 nucleotides
Pf1N1B4_5310 - hypothetical protein, at 60,819 to 61,040
_5310
Pf1N1B4_5311 - hypothetical protein, at 61,965 to 62,252
_5311
Pf1N1B4_5312 - Putative activity regulator of membrane protease YbbK, at 62,495 to 62,932
_5312
Pf1N1B4_5313 - Putative stomatin/prohibitin-family membrane protease subunit YbbK, at 62,973 to 63,839
_5313
Position (kb) 61
62
63 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 61.074 kb on + strand at 61.075 kb on - strand at 61.098 kb on - strand at 61.222 kb on - strand at 61.421 kb on - strand at 61.421 kb on - strand at 61.448 kb on - strand at 61.464 kb on + strand at 61.496 kb on + strand at 61.534 kb on + strand at 61.605 kb on - strand at 61.675 kb on + strand at 61.675 kb on + strand at 61.676 kb on - strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.679 kb on + strand at 61.680 kb on - strand at 61.714 kb on + strand at 61.714 kb on + strand at 61.723 kb on + strand at 61.866 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.920 kb on + strand at 61.921 kb on - strand at 61.921 kb on - strand at 61.921 kb on - strand at 62.067 kb on + strand, within Pf1N1B4_5311 at 62.068 kb on - strand, within Pf1N1B4_5311 at 62.113 kb on + strand, within Pf1N1B4_5311 at 62.113 kb on + strand, within Pf1N1B4_5311 at 62.279 kb on + strand at 62.279 kb on + strand at 62.279 kb on + strand at 62.279 kb on + strand at 62.323 kb on + strand at 62.323 kb on + strand at 62.323 kb on + strand at 62.323 kb on + strand at 62.324 kb on - strand at 62.324 kb on - strand at 62.324 kb on - strand at 62.324 kb on - strand at 62.329 kb on + strand at 62.330 kb on + strand at 62.330 kb on + strand at 62.331 kb on - strand at 62.372 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.387 kb on + strand at 62.388 kb on - strand at 62.388 kb on - strand at 62.388 kb on - strand at 62.421 kb on + strand at 62.421 kb on + strand at 62.421 kb on + strand at 62.421 kb on + strand at 62.430 kb on + strand at 62.456 kb on + strand at 62.456 kb on + strand at 62.457 kb on - strand at 62.614 kb on + strand, within Pf1N1B4_5312 at 62.614 kb on + strand at 62.615 kb on - strand, within Pf1N1B4_5312 at 62.634 kb on + strand, within Pf1N1B4_5312 at 62.634 kb on + strand, within Pf1N1B4_5312 at 62.635 kb on - strand, within Pf1N1B4_5312 at 62.725 kb on + strand, within Pf1N1B4_5312 at 62.826 kb on + strand, within Pf1N1B4_5312 at 62.826 kb on + strand, within Pf1N1B4_5312 at 62.827 kb on - strand, within Pf1N1B4_5312 at 62.892 kb on + strand at 62.892 kb on + strand at 62.892 kb on + strand at 62.892 kb on + strand at 62.893 kb on - strand at 62.952 kb on + strand at 62.952 kb on + strand at 62.952 kb on + strand at 62.968 kb on + strand at 62.968 kb on + strand at 62.968 kb on + strand at 62.968 kb on + strand at 62.971 kb on + strand at 62.971 kb on + strand at 62.972 kb on - strand at 63.131 kb on - strand, within Pf1N1B4_5313
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 61,074 + +1.3 61,075 - +0.0 61,098 - +0.3 61,222 - +1.0 61,421 - -0.4 61,421 - +0.0 61,448 - +0.8 61,464 + +0.8 61,496 + -0.1 61,534 + -1.3 61,605 - +0.0 61,675 + +0.1 61,675 + -0.4 61,676 - +0.2 61,679 + -0.1 61,679 + -0.1 61,679 + +0.5 61,679 + -0.1 61,679 + -0.6 61,679 + +2.6 61,679 + -0.7 61,679 + -0.6 61,679 + -1.6 61,680 - -2.0 61,714 + +0.8 61,714 + -0.3 61,723 + +1.0 61,866 + +0.3 61,920 + -1.3 61,920 + +0.0 61,920 + +1.4 61,920 + -0.3 61,920 + +1.4 61,920 + -2.3 61,920 + -0.3 61,920 + +0.4 61,920 + +0.2 61,920 + +1.2 61,920 + +3.6 61,920 + -2.0 61,920 + -1.3 61,920 + +0.4 61,920 + -1.6 61,921 - +1.6 61,921 - -0.1 61,921 - +1.0 62,067 + Pf1N1B4_5311 0.35 -0.3 62,068 - Pf1N1B4_5311 0.36 -1.2 62,113 + Pf1N1B4_5311 0.51 +0.5 62,113 + Pf1N1B4_5311 0.51 -2.3 62,279 + +0.7 62,279 + +0.0 62,279 + +0.3 62,279 + -0.7 62,323 + -0.2 62,323 + -0.3 62,323 + -0.9 62,323 + -0.7 62,324 - +0.3 62,324 - -0.3 62,324 - +0.1 62,324 - -0.6 62,329 + +0.2 62,330 + +0.6 62,330 + +0.0 62,331 - -1.0 62,372 + -2.0 62,387 + +0.1 62,387 + -1.2 62,387 + -1.1 62,387 + +0.0 62,387 + +0.6 62,387 + +0.0 62,387 + +0.2 62,387 + -0.6 62,387 + -0.3 62,387 + +0.2 62,388 - +0.2 62,388 - -3.1 62,388 - +1.0 62,421 + -0.4 62,421 + -0.6 62,421 + -1.0 62,421 + +2.0 62,430 + -1.3 62,456 + +0.0 62,456 + -1.0 62,457 - -1.3 62,614 + Pf1N1B4_5312 0.27 -2.0 62,614 + +0.0 62,615 - Pf1N1B4_5312 0.27 -0.5 62,634 + Pf1N1B4_5312 0.32 +0.4 62,634 + Pf1N1B4_5312 0.32 +0.6 62,635 - Pf1N1B4_5312 0.32 +1.0 62,725 + Pf1N1B4_5312 0.53 +0.0 62,826 + Pf1N1B4_5312 0.76 +0.8 62,826 + Pf1N1B4_5312 0.76 -1.0 62,827 - Pf1N1B4_5312 0.76 +0.7 62,892 + -1.8 62,892 + -1.8 62,892 + -1.6 62,892 + -0.1 62,893 - +0.0 62,952 + -0.3 62,952 + +0.6 62,952 + -2.6 62,968 + +0.2 62,968 + +0.9 62,968 + -1.8 62,968 + +0.0 62,971 + +1.4 62,971 + -0.8 62,972 - -1.0 63,131 - Pf1N1B4_5313 0.18 +0.3
Or see this region's nucleotide sequence