Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_521 and Pf1N1B4_522 are separated by 189 nucleotides Pf1N1B4_522 and Pf1N1B4_523 are separated by 114 nucleotides Pf1N1B4_523 and Pf1N1B4_524 are separated by 4 nucleotides
Pf1N1B4_521 - ABC transporter, transmembrane region:ABC transporter:Peptidase C39, bacteriocin processing, at 618,939 to 621,098
_521
Pf1N1B4_522 - HlyD family secretion protein, at 621,288 to 622,652
_522
Pf1N1B4_523 - FIG00955751: hypothetical protein, at 622,767 to 623,360
_523
Pf1N1B4_524 - Transcriptional regulator GbuR, at 623,365 to 624,258
_524
Position (kb) 621
622
623 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 620.413 kb on - strand, within Pf1N1B4_521 at 620.413 kb on - strand, within Pf1N1B4_521 at 620.413 kb on - strand, within Pf1N1B4_521 at 620.539 kb on - strand, within Pf1N1B4_521 at 620.539 kb on - strand, within Pf1N1B4_521 at 620.623 kb on - strand, within Pf1N1B4_521 at 620.689 kb on - strand, within Pf1N1B4_521 at 620.689 kb on - strand, within Pf1N1B4_521 at 620.843 kb on - strand, within Pf1N1B4_521 at 621.183 kb on + strand at 621.203 kb on + strand at 621.203 kb on + strand at 621.324 kb on + strand at 621.324 kb on + strand at 621.669 kb on + strand, within Pf1N1B4_522 at 621.669 kb on + strand, within Pf1N1B4_522 at 621.786 kb on + strand, within Pf1N1B4_522 at 622.392 kb on + strand, within Pf1N1B4_522 at 622.398 kb on + strand at 622.407 kb on + strand, within Pf1N1B4_522 at 622.408 kb on - strand, within Pf1N1B4_522 at 622.480 kb on - strand, within Pf1N1B4_522 at 622.480 kb on - strand at 622.571 kb on + strand at 622.571 kb on + strand at 622.571 kb on + strand at 622.599 kb on + strand at 622.599 kb on + strand at 622.608 kb on - strand at 623.062 kb on - strand, within Pf1N1B4_523 at 623.083 kb on + strand, within Pf1N1B4_523 at 623.083 kb on + strand, within Pf1N1B4_523 at 623.084 kb on - strand, within Pf1N1B4_523 at 623.366 kb on + strand at 623.366 kb on + strand at 623.367 kb on - strand at 623.367 kb on - strand at 623.494 kb on + strand, within Pf1N1B4_524 at 623.494 kb on + strand, within Pf1N1B4_524 at 623.494 kb on + strand, within Pf1N1B4_524 at 623.525 kb on + strand, within Pf1N1B4_524 at 623.541 kb on - strand, within Pf1N1B4_524 at 623.549 kb on + strand, within Pf1N1B4_524 at 623.549 kb on + strand, within Pf1N1B4_524 at 623.550 kb on - strand, within Pf1N1B4_524 at 623.550 kb on - strand, within Pf1N1B4_524 at 623.550 kb on - strand, within Pf1N1B4_524 at 623.550 kb on - strand, within Pf1N1B4_524 at 623.586 kb on + strand, within Pf1N1B4_524 at 623.586 kb on + strand, within Pf1N1B4_524 at 623.586 kb on + strand, within Pf1N1B4_524 at 623.586 kb on + strand, within Pf1N1B4_524 at 623.587 kb on - strand, within Pf1N1B4_524
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 620,413 - Pf1N1B4_521 0.68 +0.9 620,413 - Pf1N1B4_521 0.68 -0.5 620,413 - Pf1N1B4_521 0.68 +0.1 620,539 - Pf1N1B4_521 0.74 +1.9 620,539 - Pf1N1B4_521 0.74 +1.8 620,623 - Pf1N1B4_521 0.78 -1.1 620,689 - Pf1N1B4_521 0.81 +0.9 620,689 - Pf1N1B4_521 0.81 +2.5 620,843 - Pf1N1B4_521 0.88 -0.4 621,183 + +1.5 621,203 + +0.1 621,203 + -2.1 621,324 + +0.9 621,324 + -1.2 621,669 + Pf1N1B4_522 0.28 +0.3 621,669 + Pf1N1B4_522 0.28 -0.5 621,786 + Pf1N1B4_522 0.36 -0.4 622,392 + Pf1N1B4_522 0.81 +0.9 622,398 + +0.9 622,407 + Pf1N1B4_522 0.82 -2.3 622,408 - Pf1N1B4_522 0.82 +0.2 622,480 - Pf1N1B4_522 0.87 -1.5 622,480 - +0.2 622,571 + +3.7 622,571 + +1.3 622,571 + +1.9 622,599 + -2.1 622,599 + -0.1 622,608 - -0.9 623,062 - Pf1N1B4_523 0.50 +2.5 623,083 + Pf1N1B4_523 0.53 +0.4 623,083 + Pf1N1B4_523 0.53 -1.9 623,084 - Pf1N1B4_523 0.53 -0.3 623,366 + +2.2 623,366 + -0.8 623,367 - -0.7 623,367 - -0.1 623,494 + Pf1N1B4_524 0.14 -0.5 623,494 + Pf1N1B4_524 0.14 -0.4 623,494 + Pf1N1B4_524 0.14 -0.1 623,525 + Pf1N1B4_524 0.18 +1.5 623,541 - Pf1N1B4_524 0.20 +0.8 623,549 + Pf1N1B4_524 0.21 +0.3 623,549 + Pf1N1B4_524 0.21 -0.7 623,550 - Pf1N1B4_524 0.21 +2.4 623,550 - Pf1N1B4_524 0.21 -1.6 623,550 - Pf1N1B4_524 0.21 +1.1 623,550 - Pf1N1B4_524 0.21 -2.4 623,586 + Pf1N1B4_524 0.25 -0.2 623,586 + Pf1N1B4_524 0.25 -0.4 623,586 + Pf1N1B4_524 0.25 +0.9 623,586 + Pf1N1B4_524 0.25 +0.3 623,587 - Pf1N1B4_524 0.25 -0.1
Or see this region's nucleotide sequence