Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5094 and Pf1N1B4_5095 are separated by 80 nucleotides Pf1N1B4_5095 and Pf1N1B4_5096 are separated by 357 nucleotides
Pf1N1B4_5094 - Methyl-accepting chemotaxis protein, at 5,647,912 to 5,648,736
_5094
Pf1N1B4_5095 - hypothetical protein, at 5,648,817 to 5,649,380
_5095
Pf1N1B4_5096 - Nitrate ABC transporter, nitrate-binding protein, at 5,649,738 to 5,651,024
_5096
Position (kb)
5648
5649
5650 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5647.826 kb on + strand at 5647.827 kb on - strand at 5647.827 kb on - strand at 5647.833 kb on - strand at 5647.949 kb on + strand at 5648.236 kb on - strand, within Pf1N1B4_5094 at 5648.547 kb on + strand, within Pf1N1B4_5094 at 5648.548 kb on - strand, within Pf1N1B4_5094 at 5648.548 kb on - strand, within Pf1N1B4_5094 at 5648.667 kb on + strand at 5648.741 kb on - strand at 5648.952 kb on + strand, within Pf1N1B4_5095 at 5648.984 kb on - strand, within Pf1N1B4_5095 at 5649.200 kb on - strand, within Pf1N1B4_5095 at 5649.200 kb on - strand, within Pf1N1B4_5095 at 5649.205 kb on - strand, within Pf1N1B4_5095 at 5649.385 kb on + strand at 5649.385 kb on + strand at 5649.385 kb on + strand at 5649.386 kb on - strand at 5649.386 kb on - strand at 5649.427 kb on + strand at 5649.487 kb on + strand at 5649.959 kb on - strand, within Pf1N1B4_5096 at 5649.991 kb on - strand at 5650.095 kb on + strand, within Pf1N1B4_5096 at 5650.095 kb on + strand, within Pf1N1B4_5096 at 5650.096 kb on - strand, within Pf1N1B4_5096 at 5650.309 kb on - strand, within Pf1N1B4_5096
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 5,647,826 + -0.6 5,647,827 - -1.2 5,647,827 - -0.5 5,647,833 - -0.2 5,647,949 + +0.8 5,648,236 - Pf1N1B4_5094 0.39 -0.9 5,648,547 + Pf1N1B4_5094 0.77 -0.0 5,648,548 - Pf1N1B4_5094 0.77 -0.6 5,648,548 - Pf1N1B4_5094 0.77 -0.0 5,648,667 + -1.6 5,648,741 - +0.4 5,648,952 + Pf1N1B4_5095 0.24 +0.8 5,648,984 - Pf1N1B4_5095 0.30 -2.1 5,649,200 - Pf1N1B4_5095 0.68 -1.3 5,649,200 - Pf1N1B4_5095 0.68 -0.6 5,649,205 - Pf1N1B4_5095 0.69 -3.3 5,649,385 + -0.6 5,649,385 + -1.2 5,649,385 + -1.8 5,649,386 - -0.5 5,649,386 - -1.2 5,649,427 + +0.3 5,649,487 + -1.4 5,649,959 - Pf1N1B4_5096 0.17 +0.5 5,649,991 - +0.4 5,650,095 + Pf1N1B4_5096 0.28 +0.2 5,650,095 + Pf1N1B4_5096 0.28 -1.6 5,650,096 - Pf1N1B4_5096 0.28 +1.3 5,650,309 - Pf1N1B4_5096 0.44 +0.9
Or see this region's nucleotide sequence