Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5082 and Pf1N1B4_5083 are separated by 24 nucleotides Pf1N1B4_5083 and Pf1N1B4_5084 are separated by 2 nucleotides Pf1N1B4_5084 and Pf1N1B4_5085 are separated by 139 nucleotides
Pf1N1B4_5082 - Capsular polysaccharide synthesis enzyme CpsB, at 5,631,074 to 5,632,228
_5082
Pf1N1B4_5083 - Capsular polysaccharide synthesis enzyme CpsC, polysaccharide export, at 5,632,253 to 5,632,774
_5083
Pf1N1B4_5084 - Capsular polysaccharide synthesis enzyme CpsD, exopolysaccharide synthesis, at 5,632,777 to 5,635,023
_5084
Pf1N1B4_5085 - Capsular polysaccharide synthesis enzyme CpsA, sugar transferase, at 5,635,163 to 5,636,554
_5085
Position (kb)
5632
5633
5634
5635
5636 Strain fitness (log2 ratio)
-2
-1
0
1 at 5631.812 kb on + strand, within Pf1N1B4_5082 at 5631.900 kb on - strand, within Pf1N1B4_5082 at 5632.089 kb on - strand at 5632.089 kb on - strand, within Pf1N1B4_5082 at 5632.089 kb on - strand, within Pf1N1B4_5082 at 5632.219 kb on - strand at 5632.342 kb on - strand, within Pf1N1B4_5083 at 5632.342 kb on - strand, within Pf1N1B4_5083 at 5632.554 kb on - strand, within Pf1N1B4_5083 at 5632.773 kb on - strand at 5632.786 kb on - strand at 5632.799 kb on - strand at 5633.072 kb on - strand at 5633.363 kb on + strand, within Pf1N1B4_5084 at 5633.643 kb on + strand at 5633.679 kb on - strand, within Pf1N1B4_5084 at 5633.925 kb on - strand, within Pf1N1B4_5084 at 5633.981 kb on + strand, within Pf1N1B4_5084 at 5633.982 kb on - strand, within Pf1N1B4_5084 at 5634.447 kb on - strand, within Pf1N1B4_5084 at 5634.844 kb on + strand at 5634.845 kb on - strand at 5634.882 kb on - strand at 5635.010 kb on - strand at 5635.022 kb on - strand at 5635.065 kb on - strand at 5635.065 kb on - strand at 5635.092 kb on + strand at 5635.092 kb on + strand at 5635.346 kb on + strand, within Pf1N1B4_5085 at 5635.551 kb on - strand, within Pf1N1B4_5085 at 5635.627 kb on + strand, within Pf1N1B4_5085 at 5635.627 kb on + strand, within Pf1N1B4_5085 at 5635.628 kb on - strand, within Pf1N1B4_5085 at 5635.773 kb on + strand, within Pf1N1B4_5085 at 5635.773 kb on + strand, within Pf1N1B4_5085 at 5635.785 kb on - strand, within Pf1N1B4_5085 at 5635.785 kb on - strand, within Pf1N1B4_5085 at 5635.830 kb on - strand, within Pf1N1B4_5085 at 5635.937 kb on + strand, within Pf1N1B4_5085 at 5636.023 kb on + strand, within Pf1N1B4_5085
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 5,631,812 + Pf1N1B4_5082 0.64 -0.4 5,631,900 - Pf1N1B4_5082 0.72 -0.3 5,632,089 - +1.6 5,632,089 - Pf1N1B4_5082 0.88 +0.4 5,632,089 - Pf1N1B4_5082 0.88 +0.4 5,632,219 - -0.4 5,632,342 - Pf1N1B4_5083 0.17 -1.2 5,632,342 - Pf1N1B4_5083 0.17 +0.8 5,632,554 - Pf1N1B4_5083 0.58 -0.3 5,632,773 - +0.1 5,632,786 - -0.7 5,632,799 - +1.1 5,633,072 - -0.6 5,633,363 + Pf1N1B4_5084 0.26 -0.1 5,633,643 + -0.6 5,633,679 - Pf1N1B4_5084 0.40 -0.3 5,633,925 - Pf1N1B4_5084 0.51 -1.1 5,633,981 + Pf1N1B4_5084 0.54 +0.1 5,633,982 - Pf1N1B4_5084 0.54 -1.1 5,634,447 - Pf1N1B4_5084 0.74 -1.9 5,634,844 + -0.6 5,634,845 - +0.1 5,634,882 - +0.8 5,635,010 - -0.3 5,635,022 - -0.9 5,635,065 - +1.0 5,635,065 - -0.8 5,635,092 + +0.4 5,635,092 + +0.4 5,635,346 + Pf1N1B4_5085 0.13 -1.6 5,635,551 - Pf1N1B4_5085 0.28 -0.9 5,635,627 + Pf1N1B4_5085 0.33 +0.8 5,635,627 + Pf1N1B4_5085 0.33 -0.6 5,635,628 - Pf1N1B4_5085 0.33 +0.5 5,635,773 + Pf1N1B4_5085 0.44 -2.1 5,635,773 + Pf1N1B4_5085 0.44 +0.0 5,635,785 - Pf1N1B4_5085 0.45 -0.4 5,635,785 - Pf1N1B4_5085 0.45 -1.3 5,635,830 - Pf1N1B4_5085 0.48 -1.6 5,635,937 + Pf1N1B4_5085 0.56 -0.2 5,636,023 + Pf1N1B4_5085 0.62 +0.7
Or see this region's nucleotide sequence