Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4974 and Pf1N1B4_4975 are separated by 826 nucleotides Pf1N1B4_4975 and Pf1N1B4_4976 are separated by 145 nucleotides
Pf1N1B4_4974 - Protease subunit of ATP-dependent Clp proteases, at 5,513,420 to 5,513,977
_4974
Pf1N1B4_4975 - hypothetical protein, at 5,514,804 to 5,515,145
_4975
Pf1N1B4_4976 - D-serine/D-alanine/glycine transporter, at 5,515,291 to 5,516,712
_4976
Position (kb)
5514
5515
5516 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5513.821 kb on - strand, within Pf1N1B4_4974 at 5514.003 kb on + strand at 5514.024 kb on + strand at 5514.025 kb on - strand at 5514.084 kb on - strand at 5514.094 kb on + strand at 5514.096 kb on + strand at 5514.118 kb on + strand at 5514.147 kb on + strand at 5514.147 kb on + strand at 5514.147 kb on + strand at 5514.147 kb on + strand at 5514.147 kb on + strand at 5514.147 kb on + strand at 5514.147 kb on + strand at 5514.220 kb on - strand at 5514.363 kb on + strand at 5514.558 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.580 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.679 kb on + strand at 5514.680 kb on - strand at 5514.691 kb on + strand at 5514.691 kb on + strand at 5514.691 kb on + strand at 5514.691 kb on + strand at 5514.695 kb on - strand at 5514.695 kb on - strand at 5514.814 kb on + strand at 5514.814 kb on + strand at 5514.814 kb on + strand at 5514.815 kb on - strand at 5514.815 kb on - strand at 5514.919 kb on - strand, within Pf1N1B4_4975 at 5515.041 kb on + strand, within Pf1N1B4_4975 at 5515.041 kb on + strand, within Pf1N1B4_4975 at 5515.041 kb on + strand at 5515.042 kb on - strand, within Pf1N1B4_4975 at 5515.074 kb on + strand, within Pf1N1B4_4975 at 5515.074 kb on + strand, within Pf1N1B4_4975 at 5515.074 kb on + strand at 5515.074 kb on + strand, within Pf1N1B4_4975 at 5515.160 kb on + strand at 5515.548 kb on - strand, within Pf1N1B4_4976 at 5515.572 kb on + strand, within Pf1N1B4_4976 at 5515.573 kb on - strand, within Pf1N1B4_4976 at 5515.767 kb on + strand, within Pf1N1B4_4976 at 5515.767 kb on + strand at 5516.038 kb on + strand, within Pf1N1B4_4976
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 5,513,821 - Pf1N1B4_4974 0.72 +0.2 5,514,003 + -0.4 5,514,024 + +0.4 5,514,025 - +1.8 5,514,084 - -0.1 5,514,094 + +0.6 5,514,096 + -0.3 5,514,118 + -1.2 5,514,147 + -1.3 5,514,147 + -1.3 5,514,147 + +0.4 5,514,147 + +0.5 5,514,147 + +1.6 5,514,147 + -0.6 5,514,147 + +0.5 5,514,220 - -0.3 5,514,363 + -1.6 5,514,558 + -0.6 5,514,580 + -2.6 5,514,580 + +2.4 5,514,580 + +0.7 5,514,580 + +1.1 5,514,580 + -1.9 5,514,580 + +1.9 5,514,679 + +0.4 5,514,679 + -0.2 5,514,679 + +0.8 5,514,679 + +1.2 5,514,679 + +1.8 5,514,679 + +1.0 5,514,680 - +0.1 5,514,691 + +0.5 5,514,691 + +2.0 5,514,691 + +0.6 5,514,691 + -1.8 5,514,695 - -0.3 5,514,695 - +0.8 5,514,814 + +0.6 5,514,814 + +1.2 5,514,814 + +0.4 5,514,815 - -0.7 5,514,815 - +0.1 5,514,919 - Pf1N1B4_4975 0.34 -0.3 5,515,041 + Pf1N1B4_4975 0.69 +0.7 5,515,041 + Pf1N1B4_4975 0.69 +1.1 5,515,041 + +0.4 5,515,042 - Pf1N1B4_4975 0.70 +0.4 5,515,074 + Pf1N1B4_4975 0.79 +0.9 5,515,074 + Pf1N1B4_4975 0.79 -1.6 5,515,074 + +1.4 5,515,074 + Pf1N1B4_4975 0.79 +1.4 5,515,160 + +0.4 5,515,548 - Pf1N1B4_4976 0.18 -0.3 5,515,572 + Pf1N1B4_4976 0.20 -0.1 5,515,573 - Pf1N1B4_4976 0.20 -0.6 5,515,767 + Pf1N1B4_4976 0.33 -0.8 5,515,767 + +0.1 5,516,038 + Pf1N1B4_4976 0.53 -1.6
Or see this region's nucleotide sequence