Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_495

Experiment: Nalidixic 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_494 and Pf1N1B4_495 are separated by 30 nucleotidesPf1N1B4_495 and Pf1N1B4_496 overlap by 4 nucleotides Pf1N1B4_494 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 586,319 to 587,281 _494 Pf1N1B4_495 - Flp pilus assembly protein TadB, at 587,312 to 588,298 _495 Pf1N1B4_496 - Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily, at 588,295 to 589,737 _496 Position (kb) 587 588 589Strain fitness (log2 ratio) -2 -1 0 1 2 3at 586.406 kb on + strandat 586.407 kb on - strandat 586.407 kb on - strandat 586.453 kb on + strand, within Pf1N1B4_494at 586.479 kb on + strand, within Pf1N1B4_494at 586.479 kb on + strand, within Pf1N1B4_494at 586.479 kb on + strand, within Pf1N1B4_494at 586.479 kb on + strand, within Pf1N1B4_494at 586.480 kb on - strand, within Pf1N1B4_494at 586.480 kb on - strand, within Pf1N1B4_494at 586.480 kb on - strand, within Pf1N1B4_494at 586.480 kb on - strand, within Pf1N1B4_494at 586.480 kb on - strand, within Pf1N1B4_494at 586.520 kb on - strand, within Pf1N1B4_494at 586.550 kb on + strand, within Pf1N1B4_494at 586.550 kb on + strand, within Pf1N1B4_494at 586.550 kb on + strand, within Pf1N1B4_494at 586.551 kb on - strandat 586.551 kb on - strand, within Pf1N1B4_494at 586.551 kb on - strand, within Pf1N1B4_494at 586.551 kb on - strand, within Pf1N1B4_494at 586.551 kb on - strand, within Pf1N1B4_494at 586.551 kb on - strand, within Pf1N1B4_494at 586.551 kb on - strand, within Pf1N1B4_494at 586.803 kb on + strand, within Pf1N1B4_494at 586.803 kb on + strand, within Pf1N1B4_494at 586.803 kb on + strand, within Pf1N1B4_494at 586.804 kb on - strand, within Pf1N1B4_494at 586.804 kb on - strand, within Pf1N1B4_494at 586.893 kb on - strand, within Pf1N1B4_494at 586.948 kb on - strand, within Pf1N1B4_494at 586.948 kb on - strand, within Pf1N1B4_494at 587.095 kb on + strand, within Pf1N1B4_494at 587.096 kb on - strand, within Pf1N1B4_494at 587.096 kb on - strandat 587.096 kb on - strand, within Pf1N1B4_494at 587.103 kb on - strandat 587.195 kb on - strandat 587.271 kb on - strandat 587.289 kb on - strandat 587.313 kb on + strandat 587.313 kb on + strandat 587.397 kb on - strandat 587.424 kb on + strand, within Pf1N1B4_495at 587.424 kb on + strand, within Pf1N1B4_495at 587.424 kb on + strand, within Pf1N1B4_495at 587.424 kb on + strand, within Pf1N1B4_495at 587.424 kb on + strand, within Pf1N1B4_495at 587.424 kb on + strand, within Pf1N1B4_495at 587.424 kb on + strand, within Pf1N1B4_495at 587.424 kb on + strand, within Pf1N1B4_495at 587.424 kb on + strand, within Pf1N1B4_495at 587.425 kb on - strand, within Pf1N1B4_495at 587.425 kb on - strandat 587.425 kb on - strand, within Pf1N1B4_495at 587.425 kb on - strand, within Pf1N1B4_495at 587.438 kb on - strand, within Pf1N1B4_495at 587.609 kb on - strand, within Pf1N1B4_495at 587.651 kb on - strand, within Pf1N1B4_495at 587.694 kb on + strand, within Pf1N1B4_495at 587.694 kb on + strand, within Pf1N1B4_495at 587.695 kb on - strand, within Pf1N1B4_495at 587.695 kb on - strand, within Pf1N1B4_495at 587.744 kb on - strand, within Pf1N1B4_495at 587.744 kb on - strand, within Pf1N1B4_495at 587.744 kb on - strandat 587.990 kb on + strand, within Pf1N1B4_495at 587.990 kb on + strand, within Pf1N1B4_495at 588.102 kb on + strand, within Pf1N1B4_495at 588.103 kb on - strand, within Pf1N1B4_495at 588.140 kb on - strand, within Pf1N1B4_495at 588.156 kb on - strand, within Pf1N1B4_495at 588.207 kb on - strandat 588.207 kb on - strandat 588.207 kb on - strandat 588.266 kb on + strandat 588.267 kb on - strandat 588.290 kb on - strandat 588.850 kb on + strand, within Pf1N1B4_496at 588.851 kb on - strand, within Pf1N1B4_496at 589.298 kb on + strand, within Pf1N1B4_496at 589.298 kb on + strand, within Pf1N1B4_496

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Per-strain Table

Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml
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586,406 + -0.1
586,407 - +0.2
586,407 - -0.7
586,453 + Pf1N1B4_494 0.14 +1.3
586,479 + Pf1N1B4_494 0.17 +1.3
586,479 + Pf1N1B4_494 0.17 +0.7
586,479 + Pf1N1B4_494 0.17 -0.1
586,479 + Pf1N1B4_494 0.17 -1.4
586,480 - Pf1N1B4_494 0.17 +0.7
586,480 - Pf1N1B4_494 0.17 -0.5
586,480 - Pf1N1B4_494 0.17 -0.2
586,480 - Pf1N1B4_494 0.17 +1.3
586,480 - Pf1N1B4_494 0.17 +0.3
586,520 - Pf1N1B4_494 0.21 +0.9
586,550 + Pf1N1B4_494 0.24 -1.9
586,550 + Pf1N1B4_494 0.24 +1.1
586,550 + Pf1N1B4_494 0.24 +1.9
586,551 - -0.7
586,551 - Pf1N1B4_494 0.24 -0.2
586,551 - Pf1N1B4_494 0.24 +0.6
586,551 - Pf1N1B4_494 0.24 +0.7
586,551 - Pf1N1B4_494 0.24 -1.9
586,551 - Pf1N1B4_494 0.24 +1.4
586,551 - Pf1N1B4_494 0.24 -0.1
586,803 + Pf1N1B4_494 0.50 -0.5
586,803 + Pf1N1B4_494 0.50 +0.3
586,803 + Pf1N1B4_494 0.50 +0.6
586,804 - Pf1N1B4_494 0.50 +0.0
586,804 - Pf1N1B4_494 0.50 +0.5
586,893 - Pf1N1B4_494 0.60 +0.4
586,948 - Pf1N1B4_494 0.65 +0.3
586,948 - Pf1N1B4_494 0.65 +0.3
587,095 + Pf1N1B4_494 0.81 -0.1
587,096 - Pf1N1B4_494 0.81 -0.4
587,096 - -1.7
587,096 - Pf1N1B4_494 0.81 -0.1
587,103 - -1.1
587,195 - -0.7
587,271 - -0.1
587,289 - +0.9
587,313 + -2.1
587,313 + +1.9
587,397 - -0.3
587,424 + Pf1N1B4_495 0.11 +1.4
587,424 + Pf1N1B4_495 0.11 +1.0
587,424 + Pf1N1B4_495 0.11 +0.9
587,424 + Pf1N1B4_495 0.11 +2.7
587,424 + Pf1N1B4_495 0.11 -1.1
587,424 + Pf1N1B4_495 0.11 -1.7
587,424 + Pf1N1B4_495 0.11 +0.5
587,424 + Pf1N1B4_495 0.11 +1.4
587,424 + Pf1N1B4_495 0.11 +2.2
587,425 - Pf1N1B4_495 0.11 +1.2
587,425 - -0.7
587,425 - Pf1N1B4_495 0.11 -0.7
587,425 - Pf1N1B4_495 0.11 -0.8
587,438 - Pf1N1B4_495 0.13 +0.9
587,609 - Pf1N1B4_495 0.30 -0.1
587,651 - Pf1N1B4_495 0.34 +1.2
587,694 + Pf1N1B4_495 0.39 +0.9
587,694 + Pf1N1B4_495 0.39 -2.3
587,695 - Pf1N1B4_495 0.39 -1.1
587,695 - Pf1N1B4_495 0.39 -1.3
587,744 - Pf1N1B4_495 0.44 +1.5
587,744 - Pf1N1B4_495 0.44 +2.9
587,744 - -1.9
587,990 + Pf1N1B4_495 0.69 -0.1
587,990 + Pf1N1B4_495 0.69 -1.1
588,102 + Pf1N1B4_495 0.80 -0.5
588,103 - Pf1N1B4_495 0.80 -0.3
588,140 - Pf1N1B4_495 0.84 -2.4
588,156 - Pf1N1B4_495 0.86 +1.6
588,207 - -0.2
588,207 - +1.1
588,207 - -0.9
588,266 + -0.1
588,267 - +2.1
588,290 - -0.1
588,850 + Pf1N1B4_496 0.38 -0.7
588,851 - Pf1N1B4_496 0.39 -0.7
589,298 + Pf1N1B4_496 0.70 +0.6
589,298 + Pf1N1B4_496 0.70 +0.1

Or see this region's nucleotide sequence