Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4764 and Pf1N1B4_4765 are separated by 61 nucleotides Pf1N1B4_4765 and Pf1N1B4_4766 overlap by 8 nucleotides Pf1N1B4_4766 and Pf1N1B4_4767 are separated by 165 nucleotides
Pf1N1B4_4764 - 2-ketogluconate transporter, at 5,260,243 to 5,261,529
_4764
Pf1N1B4_4765 - 2-ketogluconate kinase (EC 2.7.1.13), at 5,261,591 to 5,262,610
_4765
Pf1N1B4_4766 - Epimerase KguE, at 5,262,603 to 5,263,385
_4766
Pf1N1B4_4767 - 2-ketogluconate utilization repressor PtxS, at 5,263,551 to 5,264,576
_4767
Position (kb)
5261
5262
5263 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5260.593 kb on - strand at 5260.593 kb on - strand, within Pf1N1B4_4764 at 5260.700 kb on + strand, within Pf1N1B4_4764 at 5260.981 kb on - strand, within Pf1N1B4_4764 at 5261.315 kb on + strand at 5261.315 kb on + strand at 5261.315 kb on + strand, within Pf1N1B4_4764 at 5261.316 kb on - strand, within Pf1N1B4_4764 at 5261.435 kb on + strand at 5261.436 kb on - strand at 5261.465 kb on + strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.529 kb on + strand at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.962 kb on - strand, within Pf1N1B4_4765 at 5262.291 kb on + strand at 5262.291 kb on + strand, within Pf1N1B4_4765 at 5262.701 kb on - strand, within Pf1N1B4_4766 at 5262.897 kb on - strand, within Pf1N1B4_4766 at 5263.190 kb on - strand at 5263.388 kb on + strand at 5263.389 kb on + strand at 5263.429 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 5,260,593 - +1.0 5,260,593 - Pf1N1B4_4764 0.27 +1.0 5,260,700 + Pf1N1B4_4764 0.36 +0.1 5,260,981 - Pf1N1B4_4764 0.57 +0.2 5,261,315 + +1.4 5,261,315 + +0.4 5,261,315 + Pf1N1B4_4764 0.83 +0.4 5,261,316 - Pf1N1B4_4764 0.83 -3.3 5,261,435 + -1.9 5,261,436 - -1.9 5,261,465 + -0.1 5,261,493 - +1.4 5,261,493 - +0.2 5,261,493 - +1.0 5,261,493 - +0.4 5,261,493 - +0.5 5,261,493 - +0.1 5,261,493 - -1.6 5,261,529 + -0.9 5,261,908 - Pf1N1B4_4765 0.31 -0.4 5,261,908 - Pf1N1B4_4765 0.31 +0.2 5,261,908 - Pf1N1B4_4765 0.31 -0.6 5,261,962 - Pf1N1B4_4765 0.36 -2.4 5,262,291 + +0.4 5,262,291 + Pf1N1B4_4765 0.69 -1.4 5,262,701 - Pf1N1B4_4766 0.13 +0.6 5,262,897 - Pf1N1B4_4766 0.38 -0.0 5,263,190 - -1.6 5,263,388 + +2.7 5,263,389 + -1.8 5,263,429 + -0.6
Or see this region's nucleotide sequence