Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4649 and Pf1N1B4_4650 are separated by 159 nucleotides Pf1N1B4_4650 and Pf1N1B4_4651 are separated by 92 nucleotides Pf1N1B4_4651 and Pf1N1B4_4652 are separated by 70 nucleotides
Pf1N1B4_4649 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 5,128,882 to 5,129,391
_4649
Pf1N1B4_4650 - Aspartate racemase (EC 5.1.1.13), at 5,129,551 to 5,130,243
_4650
Pf1N1B4_4651 - Transcriptional regulator, AraC family, at 5,130,336 to 5,131,112
_4651
Pf1N1B4_4652 - FIG00958406: hypothetical protein, at 5,131,183 to 5,131,677
_4652
Position (kb) 5129
5130
5131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5128.842 kb on - strand at 5128.955 kb on - strand at 5128.985 kb on + strand, within Pf1N1B4_4649 at 5129.330 kb on - strand, within Pf1N1B4_4649 at 5129.532 kb on + strand at 5129.762 kb on + strand, within Pf1N1B4_4650 at 5129.763 kb on - strand, within Pf1N1B4_4650 at 5129.763 kb on - strand, within Pf1N1B4_4650 at 5129.998 kb on - strand, within Pf1N1B4_4650 at 5130.122 kb on + strand, within Pf1N1B4_4650 at 5130.123 kb on - strand, within Pf1N1B4_4650 at 5130.123 kb on - strand, within Pf1N1B4_4650 at 5130.166 kb on + strand at 5130.166 kb on + strand, within Pf1N1B4_4650 at 5130.192 kb on - strand at 5130.192 kb on - strand at 5130.257 kb on - strand at 5130.321 kb on - strand at 5130.406 kb on - strand at 5130.528 kb on + strand at 5130.530 kb on + strand, within Pf1N1B4_4651 at 5130.530 kb on + strand, within Pf1N1B4_4651 at 5130.531 kb on - strand, within Pf1N1B4_4651 at 5130.531 kb on - strand, within Pf1N1B4_4651 at 5130.531 kb on - strand, within Pf1N1B4_4651 at 5130.531 kb on - strand at 5130.607 kb on + strand, within Pf1N1B4_4651 at 5130.623 kb on - strand, within Pf1N1B4_4651 at 5130.689 kb on - strand, within Pf1N1B4_4651 at 5131.111 kb on - strand at 5131.222 kb on + strand at 5131.222 kb on + strand at 5131.222 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 5,128,842 - -0.6 5,128,955 - +0.4 5,128,985 + Pf1N1B4_4649 0.20 -0.0 5,129,330 - Pf1N1B4_4649 0.88 +1.6 5,129,532 + -1.2 5,129,762 + Pf1N1B4_4650 0.30 -0.6 5,129,763 - Pf1N1B4_4650 0.31 +1.4 5,129,763 - Pf1N1B4_4650 0.31 +0.3 5,129,998 - Pf1N1B4_4650 0.65 +1.8 5,130,122 + Pf1N1B4_4650 0.82 +1.8 5,130,123 - Pf1N1B4_4650 0.83 +0.4 5,130,123 - Pf1N1B4_4650 0.83 +1.4 5,130,166 + +0.4 5,130,166 + Pf1N1B4_4650 0.89 +1.2 5,130,192 - +0.4 5,130,192 - -2.2 5,130,257 - +0.4 5,130,321 - -0.5 5,130,406 - +1.0 5,130,528 + +0.4 5,130,530 + Pf1N1B4_4651 0.25 -0.4 5,130,530 + Pf1N1B4_4651 0.25 +1.8 5,130,531 - Pf1N1B4_4651 0.25 +0.0 5,130,531 - Pf1N1B4_4651 0.25 -0.3 5,130,531 - Pf1N1B4_4651 0.25 -2.8 5,130,531 - +0.4 5,130,607 + Pf1N1B4_4651 0.35 +0.1 5,130,623 - Pf1N1B4_4651 0.37 -0.2 5,130,689 - Pf1N1B4_4651 0.45 -1.5 5,131,111 - +0.2 5,131,222 + -0.5 5,131,222 + -1.5 5,131,222 + -0.6
Or see this region's nucleotide sequence