Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_453 and Pf1N1B4_454 are separated by 34 nucleotides Pf1N1B4_454 and Pf1N1B4_455 are separated by 13 nucleotides Pf1N1B4_455 and Pf1N1B4_456 are separated by 104 nucleotides Pf1N1B4_456 and Pf1N1B4_457 are separated by 73 nucleotides
Pf1N1B4_453 - Phosphate:acyl-ACP acyltransferase PlsX, at 544,633 to 545,613
_453
Pf1N1B4_454 - LSU ribosomal protein L32p, at 545,648 to 545,830
_454
Pf1N1B4_455 - COG1399 protein, clustered with ribosomal protein L32p, at 545,844 to 546,371
_455
Pf1N1B4_456 - FIG146278: Maf/YceF/YhdE family protein, at 546,476 to 547,054
_456
Pf1N1B4_457 - Periplasmic serine proteases (ClpP class), at 547,128 to 548,144
_457
Position (kb) 546
547
548 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 545.554 kb on - strand at 546.486 kb on + strand at 546.487 kb on - strand at 546.487 kb on - strand at 546.489 kb on + strand at 546.490 kb on - strand at 546.609 kb on + strand, within Pf1N1B4_456 at 546.610 kb on - strand at 546.750 kb on + strand, within Pf1N1B4_456 at 546.750 kb on + strand, within Pf1N1B4_456 at 546.750 kb on + strand, within Pf1N1B4_456 at 546.750 kb on + strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.751 kb on - strand, within Pf1N1B4_456 at 546.849 kb on + strand, within Pf1N1B4_456 at 546.850 kb on - strand, within Pf1N1B4_456 at 546.888 kb on - strand, within Pf1N1B4_456 at 546.908 kb on + strand, within Pf1N1B4_456 at 546.908 kb on + strand, within Pf1N1B4_456 at 547.020 kb on + strand at 547.022 kb on - strand at 547.022 kb on - strand at 547.022 kb on - strand at 547.048 kb on - strand at 547.404 kb on - strand, within Pf1N1B4_457 at 547.513 kb on + strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.518 kb on - strand, within Pf1N1B4_457 at 547.612 kb on + strand, within Pf1N1B4_457 at 547.613 kb on - strand, within Pf1N1B4_457 at 547.639 kb on + strand at 547.639 kb on + strand, within Pf1N1B4_457 at 547.639 kb on + strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.642 kb on + strand, within Pf1N1B4_457 at 547.642 kb on + strand at 547.643 kb on - strand, within Pf1N1B4_457 at 547.688 kb on + strand at 547.689 kb on - strand, within Pf1N1B4_457 at 547.733 kb on - strand, within Pf1N1B4_457 at 547.873 kb on - strand, within Pf1N1B4_457 at 547.873 kb on - strand, within Pf1N1B4_457 at 547.873 kb on - strand, within Pf1N1B4_457 at 547.874 kb on + strand, within Pf1N1B4_457 at 547.875 kb on - strand, within Pf1N1B4_457 at 547.927 kb on - strand, within Pf1N1B4_457 at 547.927 kb on - strand, within Pf1N1B4_457 at 547.964 kb on - strand, within Pf1N1B4_457 at 548.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 545,554 - -0.5 546,486 + +1.2 546,487 - +0.6 546,487 - +0.5 546,489 + +0.9 546,490 - -1.4 546,609 + Pf1N1B4_456 0.23 -1.9 546,610 - -1.4 546,750 + Pf1N1B4_456 0.47 -1.3 546,750 + Pf1N1B4_456 0.47 -1.0 546,750 + Pf1N1B4_456 0.47 -1.9 546,750 + Pf1N1B4_456 0.47 +0.9 546,751 - Pf1N1B4_456 0.47 -0.7 546,751 - Pf1N1B4_456 0.47 -1.3 546,751 - Pf1N1B4_456 0.47 -1.7 546,751 - Pf1N1B4_456 0.47 -0.9 546,751 - Pf1N1B4_456 0.47 -1.4 546,849 + Pf1N1B4_456 0.64 +0.6 546,850 - Pf1N1B4_456 0.65 -0.1 546,888 - Pf1N1B4_456 0.71 -1.0 546,908 + Pf1N1B4_456 0.75 -1.4 546,908 + Pf1N1B4_456 0.75 +0.2 547,020 + -0.7 547,022 - -1.7 547,022 - +0.2 547,022 - +0.3 547,048 - +1.1 547,404 - Pf1N1B4_457 0.27 -1.4 547,513 + Pf1N1B4_457 0.38 +0.9 547,514 - Pf1N1B4_457 0.38 -0.5 547,514 - Pf1N1B4_457 0.38 -0.5 547,514 - Pf1N1B4_457 0.38 -0.1 547,514 - Pf1N1B4_457 0.38 -0.7 547,514 - Pf1N1B4_457 0.38 -0.7 547,514 - Pf1N1B4_457 0.38 -0.7 547,518 - Pf1N1B4_457 0.38 -0.7 547,612 + Pf1N1B4_457 0.48 -1.4 547,613 - Pf1N1B4_457 0.48 +1.7 547,639 + -0.1 547,639 + Pf1N1B4_457 0.50 -1.4 547,639 + Pf1N1B4_457 0.50 -1.7 547,640 - Pf1N1B4_457 0.50 -0.1 547,640 - Pf1N1B4_457 0.50 -0.7 547,640 - Pf1N1B4_457 0.50 -2.4 547,640 - Pf1N1B4_457 0.50 -0.7 547,640 - Pf1N1B4_457 0.50 +1.1 547,640 - Pf1N1B4_457 0.50 +1.6 547,640 - Pf1N1B4_457 0.50 -0.3 547,640 - Pf1N1B4_457 0.50 -1.1 547,640 - Pf1N1B4_457 0.50 -0.1 547,640 - Pf1N1B4_457 0.50 -1.1 547,642 + Pf1N1B4_457 0.51 +1.6 547,642 + +3.5 547,643 - Pf1N1B4_457 0.51 -0.9 547,688 + +0.9 547,689 - Pf1N1B4_457 0.55 -0.1 547,733 - Pf1N1B4_457 0.59 -2.1 547,873 - Pf1N1B4_457 0.73 -0.4 547,873 - Pf1N1B4_457 0.73 -0.3 547,873 - Pf1N1B4_457 0.73 -1.4 547,874 + Pf1N1B4_457 0.73 +0.9 547,875 - Pf1N1B4_457 0.73 -0.3 547,927 - Pf1N1B4_457 0.79 +0.3 547,927 - Pf1N1B4_457 0.79 +0.4 547,964 - Pf1N1B4_457 0.82 +0.1 548,050 - -0.1
Or see this region's nucleotide sequence