Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4555 and Pf1N1B4_4556 are separated by 53 nucleotides Pf1N1B4_4556 and Pf1N1B4_4557 are separated by 2 nucleotides Pf1N1B4_4557 and Pf1N1B4_4558 are separated by 82 nucleotides Pf1N1B4_4558 and Pf1N1B4_4559 are separated by 26 nucleotides
Pf1N1B4_4555 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 5,025,483 to 5,025,602
_4555
Pf1N1B4_4556 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 5,025,656 to 5,026,363
_4556
Pf1N1B4_4557 - FIG00955691: hypothetical protein, at 5,026,366 to 5,027,649
_4557
Pf1N1B4_4558 - Outer membrane lipoprotein-sorting protein, at 5,027,732 to 5,028,568
_4558
Pf1N1B4_4559 - Predicted exporter of the RND superfamily, at 5,028,595 to 5,031,210
_4559
Position (kb)
5026
5027
5028 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5025.531 kb on + strand, within Pf1N1B4_4555 at 5025.532 kb on - strand, within Pf1N1B4_4555 at 5025.532 kb on - strand, within Pf1N1B4_4555 at 5025.532 kb on - strand, within Pf1N1B4_4555 at 5025.532 kb on - strand, within Pf1N1B4_4555 at 5025.644 kb on - strand at 5025.770 kb on + strand at 5025.772 kb on + strand at 5025.839 kb on + strand at 5025.944 kb on + strand, within Pf1N1B4_4556 at 5025.945 kb on - strand, within Pf1N1B4_4556 at 5025.945 kb on - strand, within Pf1N1B4_4556 at 5025.997 kb on + strand, within Pf1N1B4_4556 at 5025.997 kb on + strand, within Pf1N1B4_4556 at 5025.998 kb on - strand, within Pf1N1B4_4556 at 5025.998 kb on - strand, within Pf1N1B4_4556 at 5026.361 kb on + strand at 5026.361 kb on + strand at 5026.528 kb on + strand, within Pf1N1B4_4557 at 5026.570 kb on + strand, within Pf1N1B4_4557 at 5026.679 kb on - strand, within Pf1N1B4_4557 at 5026.679 kb on - strand, within Pf1N1B4_4557 at 5026.690 kb on + strand, within Pf1N1B4_4557 at 5026.690 kb on + strand, within Pf1N1B4_4557 at 5026.691 kb on - strand, within Pf1N1B4_4557 at 5026.754 kb on + strand, within Pf1N1B4_4557 at 5026.837 kb on - strand at 5027.021 kb on - strand at 5027.102 kb on - strand, within Pf1N1B4_4557 at 5027.318 kb on - strand, within Pf1N1B4_4557 at 5027.412 kb on - strand, within Pf1N1B4_4557 at 5027.603 kb on - strand at 5027.653 kb on + strand at 5027.653 kb on + strand at 5028.059 kb on + strand, within Pf1N1B4_4558 at 5028.059 kb on + strand, within Pf1N1B4_4558 at 5028.138 kb on - strand at 5028.218 kb on + strand, within Pf1N1B4_4558 at 5028.218 kb on + strand, within Pf1N1B4_4558 at 5028.218 kb on + strand, within Pf1N1B4_4558 at 5028.219 kb on - strand, within Pf1N1B4_4558 at 5028.219 kb on - strand, within Pf1N1B4_4558
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 5,025,531 + Pf1N1B4_4555 0.40 -0.7 5,025,532 - Pf1N1B4_4555 0.41 -0.4 5,025,532 - Pf1N1B4_4555 0.41 +0.0 5,025,532 - Pf1N1B4_4555 0.41 +0.7 5,025,532 - Pf1N1B4_4555 0.41 +1.0 5,025,644 - -0.6 5,025,770 + +0.7 5,025,772 + +0.5 5,025,839 + +0.0 5,025,944 + Pf1N1B4_4556 0.41 -1.1 5,025,945 - Pf1N1B4_4556 0.41 +0.3 5,025,945 - Pf1N1B4_4556 0.41 -0.1 5,025,997 + Pf1N1B4_4556 0.48 +0.7 5,025,997 + Pf1N1B4_4556 0.48 +1.0 5,025,998 - Pf1N1B4_4556 0.48 +0.5 5,025,998 - Pf1N1B4_4556 0.48 -0.5 5,026,361 + +0.9 5,026,361 + -0.5 5,026,528 + Pf1N1B4_4557 0.13 -1.3 5,026,570 + Pf1N1B4_4557 0.16 +0.2 5,026,679 - Pf1N1B4_4557 0.24 -1.1 5,026,679 - Pf1N1B4_4557 0.24 +0.1 5,026,690 + Pf1N1B4_4557 0.25 -1.7 5,026,690 + Pf1N1B4_4557 0.25 -1.0 5,026,691 - Pf1N1B4_4557 0.25 -2.5 5,026,754 + Pf1N1B4_4557 0.30 +2.8 5,026,837 - +0.5 5,027,021 - +0.5 5,027,102 - Pf1N1B4_4557 0.57 -1.7 5,027,318 - Pf1N1B4_4557 0.74 -0.7 5,027,412 - Pf1N1B4_4557 0.81 +0.0 5,027,603 - -1.5 5,027,653 + -1.1 5,027,653 + -0.8 5,028,059 + Pf1N1B4_4558 0.39 -0.1 5,028,059 + Pf1N1B4_4558 0.39 -1.1 5,028,138 - -0.5 5,028,218 + Pf1N1B4_4558 0.58 +0.5 5,028,218 + Pf1N1B4_4558 0.58 -0.9 5,028,218 + Pf1N1B4_4558 0.58 -0.5 5,028,219 - Pf1N1B4_4558 0.58 -1.1 5,028,219 - Pf1N1B4_4558 0.58 -0.2
Or see this region's nucleotide sequence