Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4515 and Pf1N1B4_4516 are separated by 0 nucleotides Pf1N1B4_4516 and Pf1N1B4_4517 are separated by 160 nucleotides
Pf1N1B4_4515 - Dihydropyrimidine dehydrogenase [NADP+] (EC 1.3.1.2), at 4,976,832 to 4,978,106
_4515
Pf1N1B4_4516 - Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism, at 4,978,107 to 4,979,474
_4516
Pf1N1B4_4517 - Dihydropyrimidinase (EC 3.5.2.2), at 4,979,635 to 4,981,074
_4517
Position (kb)
4978
4979
4980 Strain fitness (log2 ratio)
-1
0
1 at 4977.107 kb on - strand, within Pf1N1B4_4515 at 4977.122 kb on - strand at 4977.262 kb on - strand, within Pf1N1B4_4515 at 4977.607 kb on + strand at 4977.607 kb on + strand, within Pf1N1B4_4515 at 4977.608 kb on - strand at 4977.608 kb on - strand, within Pf1N1B4_4515 at 4977.641 kb on - strand, within Pf1N1B4_4515 at 4977.898 kb on - strand, within Pf1N1B4_4515 at 4978.022 kb on - strand at 4978.108 kb on + strand at 4978.109 kb on - strand at 4978.109 kb on - strand at 4978.225 kb on + strand at 4978.512 kb on + strand, within Pf1N1B4_4516 at 4978.512 kb on + strand, within Pf1N1B4_4516 at 4978.512 kb on + strand, within Pf1N1B4_4516 at 4978.512 kb on + strand, within Pf1N1B4_4516 at 4978.513 kb on - strand, within Pf1N1B4_4516 at 4978.702 kb on + strand, within Pf1N1B4_4516 at 4978.703 kb on - strand at 4979.493 kb on - strand at 4979.658 kb on + strand at 4979.659 kb on - strand at 4979.659 kb on - strand at 4979.721 kb on - strand at 4980.296 kb on + strand at 4980.369 kb on - strand, within Pf1N1B4_4517
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,977,107 - Pf1N1B4_4515 0.22 -0.8 4,977,122 - -0.5 4,977,262 - Pf1N1B4_4515 0.34 -0.2 4,977,607 + +0.5 4,977,607 + Pf1N1B4_4515 0.61 -1.5 4,977,608 - +1.5 4,977,608 - Pf1N1B4_4515 0.61 +0.3 4,977,641 - Pf1N1B4_4515 0.63 +1.3 4,977,898 - Pf1N1B4_4515 0.84 -0.8 4,978,022 - -0.7 4,978,108 + +0.2 4,978,109 - +1.5 4,978,109 - -1.5 4,978,225 + +0.3 4,978,512 + Pf1N1B4_4516 0.30 -0.3 4,978,512 + Pf1N1B4_4516 0.30 -0.8 4,978,512 + Pf1N1B4_4516 0.30 -0.8 4,978,512 + Pf1N1B4_4516 0.30 +1.2 4,978,513 - Pf1N1B4_4516 0.30 -0.9 4,978,702 + Pf1N1B4_4516 0.43 -1.4 4,978,703 - -0.5 4,979,493 - +1.1 4,979,658 + +1.1 4,979,659 - +0.6 4,979,659 - -0.2 4,979,721 - +1.2 4,980,296 + -0.1 4,980,369 - Pf1N1B4_4517 0.51 -0.8
Or see this region's nucleotide sequence