Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4416 and Pf1N1B4_4417 are separated by 464 nucleotides Pf1N1B4_4417 and Pf1N1B4_4418 overlap by 4 nucleotides Pf1N1B4_4418 and Pf1N1B4_4419 are separated by 232 nucleotides Pf1N1B4_4419 and Pf1N1B4_4420 are separated by 112 nucleotides Pf1N1B4_4420 and Pf1N1B4_4421 are separated by 68 nucleotides
Pf1N1B4_4416 - membrane protein, putative, at 4,878,920 to 4,879,435
_4416
Pf1N1B4_4417 - Transcriptional regulator, at 4,879,900 to 4,880,547
_4417
Pf1N1B4_4418 - hypothetical protein, at 4,880,544 to 4,880,705
_4418
Pf1N1B4_4419 - Organosulfonate utilization protein SsuF, at 4,880,938 to 4,881,153
_4419
Pf1N1B4_4420 - hypothetical protein, at 4,881,266 to 4,881,406
_4420
Pf1N1B4_4421 - LysR family transcriptional regulator lrhA, at 4,881,475 to 4,882,335
_4421
Position (kb)
4879
4880
4881 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4878.921 kb on + strand at 4878.922 kb on - strand at 4879.563 kb on - strand at 4879.839 kb on + strand at 4879.840 kb on - strand at 4879.840 kb on - strand at 4879.840 kb on - strand at 4879.840 kb on - strand at 4879.840 kb on - strand at 4879.840 kb on - strand at 4879.840 kb on - strand at 4879.919 kb on + strand at 4879.935 kb on - strand at 4879.935 kb on - strand at 4879.935 kb on - strand at 4879.989 kb on - strand, within Pf1N1B4_4417 at 4880.021 kb on + strand at 4880.021 kb on + strand, within Pf1N1B4_4417 at 4880.022 kb on - strand, within Pf1N1B4_4417 at 4880.022 kb on - strand, within Pf1N1B4_4417 at 4880.599 kb on - strand, within Pf1N1B4_4418 at 4880.599 kb on - strand, within Pf1N1B4_4418 at 4880.609 kb on + strand, within Pf1N1B4_4418 at 4880.617 kb on + strand, within Pf1N1B4_4418 at 4880.617 kb on + strand, within Pf1N1B4_4418 at 4880.705 kb on + strand at 4880.750 kb on - strand at 4880.993 kb on + strand, within Pf1N1B4_4419 at 4880.996 kb on + strand, within Pf1N1B4_4419 at 4880.997 kb on - strand at 4881.060 kb on + strand, within Pf1N1B4_4419 at 4881.060 kb on + strand, within Pf1N1B4_4419 at 4881.156 kb on + strand at 4881.156 kb on + strand at 4881.194 kb on + strand at 4881.194 kb on + strand at 4881.506 kb on + strand at 4881.506 kb on + strand at 4881.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,878,921 + +2.7 4,878,922 - -0.2 4,879,563 - -1.9 4,879,839 + -1.1 4,879,840 - -0.5 4,879,840 - +1.5 4,879,840 - -0.1 4,879,840 - -0.5 4,879,840 - +0.1 4,879,840 - +2.5 4,879,840 - -0.3 4,879,919 + +0.3 4,879,935 - -0.1 4,879,935 - +1.3 4,879,935 - +0.1 4,879,989 - Pf1N1B4_4417 0.14 +0.8 4,880,021 + +0.5 4,880,021 + Pf1N1B4_4417 0.19 -1.5 4,880,022 - Pf1N1B4_4417 0.19 +1.8 4,880,022 - Pf1N1B4_4417 0.19 +1.3 4,880,599 - Pf1N1B4_4418 0.34 +1.9 4,880,599 - Pf1N1B4_4418 0.34 -0.2 4,880,609 + Pf1N1B4_4418 0.40 +0.0 4,880,617 + Pf1N1B4_4418 0.45 -0.8 4,880,617 + Pf1N1B4_4418 0.45 +0.5 4,880,705 + +1.1 4,880,750 - +0.0 4,880,993 + Pf1N1B4_4419 0.25 +0.2 4,880,996 + Pf1N1B4_4419 0.27 +1.0 4,880,997 - +1.5 4,881,060 + Pf1N1B4_4419 0.56 -0.2 4,881,060 + Pf1N1B4_4419 0.56 +0.9 4,881,156 + -0.3 4,881,156 + -0.8 4,881,194 + +0.8 4,881,194 + -1.1 4,881,506 + +1.5 4,881,506 + +0.5 4,881,507 - -0.2
Or see this region's nucleotide sequence