Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4350 and Pf1N1B4_4351 overlap by 4 nucleotides Pf1N1B4_4351 and Pf1N1B4_4352 overlap by 4 nucleotides Pf1N1B4_4352 and Pf1N1B4_4353 are separated by 350 nucleotides
Pf1N1B4_4350 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 4,806,194 to 4,807,078
_4350
Pf1N1B4_4351 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 4,807,075 to 4,807,989
_4351
Pf1N1B4_4352 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 4,807,986 to 4,809,128
_4352
Pf1N1B4_4353 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94), at 4,809,479 to 4,810,240
_4353
Position (kb)
4807
4808
4809
4810 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4807.024 kb on - strand at 4807.024 kb on - strand at 4807.028 kb on - strand at 4807.445 kb on + strand, within Pf1N1B4_4351 at 4807.446 kb on - strand, within Pf1N1B4_4351 at 4807.446 kb on - strand, within Pf1N1B4_4351 at 4807.452 kb on - strand, within Pf1N1B4_4351 at 4807.452 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.664 kb on + strand, within Pf1N1B4_4351 at 4807.665 kb on - strand, within Pf1N1B4_4351 at 4807.665 kb on - strand at 4807.745 kb on + strand, within Pf1N1B4_4351 at 4807.745 kb on + strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.773 kb on - strand, within Pf1N1B4_4351 at 4807.820 kb on + strand, within Pf1N1B4_4351 at 4807.832 kb on + strand, within Pf1N1B4_4351 at 4807.841 kb on - strand at 4808.029 kb on + strand at 4808.030 kb on - strand at 4808.177 kb on + strand, within Pf1N1B4_4352 at 4808.233 kb on + strand, within Pf1N1B4_4352 at 4808.234 kb on - strand, within Pf1N1B4_4352 at 4808.234 kb on - strand, within Pf1N1B4_4352 at 4808.407 kb on + strand, within Pf1N1B4_4352 at 4808.407 kb on + strand, within Pf1N1B4_4352 at 4808.408 kb on - strand, within Pf1N1B4_4352 at 4808.408 kb on - strand, within Pf1N1B4_4352 at 4809.106 kb on + strand at 4809.106 kb on + strand at 4809.106 kb on + strand at 4809.107 kb on - strand at 4809.107 kb on - strand at 4809.176 kb on + strand at 4809.176 kb on + strand at 4809.176 kb on + strand at 4809.281 kb on - strand at 4809.376 kb on + strand at 4809.377 kb on - strand at 4809.400 kb on + strand at 4809.401 kb on - strand at 4809.403 kb on + strand at 4809.403 kb on + strand at 4809.404 kb on - strand at 4809.404 kb on - strand at 4809.455 kb on + strand at 4809.456 kb on - strand at 4809.488 kb on - strand at 4809.538 kb on + strand at 4809.546 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,807,024 - -1.9 4,807,024 - +0.1 4,807,028 - +2.6 4,807,445 + Pf1N1B4_4351 0.40 +1.6 4,807,446 - Pf1N1B4_4351 0.41 -0.6 4,807,446 - Pf1N1B4_4351 0.41 -0.7 4,807,452 - Pf1N1B4_4351 0.41 -0.9 4,807,452 - Pf1N1B4_4351 0.41 +0.6 4,807,467 - Pf1N1B4_4351 0.43 +0.1 4,807,467 - Pf1N1B4_4351 0.43 +0.1 4,807,467 - Pf1N1B4_4351 0.43 +0.5 4,807,664 + Pf1N1B4_4351 0.64 -0.2 4,807,665 - Pf1N1B4_4351 0.64 +0.6 4,807,665 - +0.6 4,807,745 + Pf1N1B4_4351 0.73 -1.4 4,807,745 + Pf1N1B4_4351 0.73 -0.4 4,807,746 - Pf1N1B4_4351 0.73 -3.0 4,807,746 - Pf1N1B4_4351 0.73 +1.3 4,807,746 - Pf1N1B4_4351 0.73 -0.4 4,807,746 - Pf1N1B4_4351 0.73 +1.7 4,807,773 - Pf1N1B4_4351 0.76 -0.6 4,807,820 + Pf1N1B4_4351 0.81 +0.3 4,807,832 + Pf1N1B4_4351 0.83 +0.1 4,807,841 - -1.0 4,808,029 + +0.6 4,808,030 - +1.3 4,808,177 + Pf1N1B4_4352 0.17 -0.7 4,808,233 + Pf1N1B4_4352 0.22 -1.0 4,808,234 - Pf1N1B4_4352 0.22 -0.1 4,808,234 - Pf1N1B4_4352 0.22 -1.4 4,808,407 + Pf1N1B4_4352 0.37 -2.0 4,808,407 + Pf1N1B4_4352 0.37 -0.2 4,808,408 - Pf1N1B4_4352 0.37 -0.4 4,808,408 - Pf1N1B4_4352 0.37 -0.7 4,809,106 + +3.2 4,809,106 + -0.0 4,809,106 + -2.9 4,809,107 - -0.4 4,809,107 - -2.8 4,809,176 + -0.3 4,809,176 + +0.6 4,809,176 + +0.9 4,809,281 - -1.2 4,809,376 + +1.2 4,809,377 - -0.0 4,809,400 + -0.0 4,809,401 - -1.8 4,809,403 + +0.6 4,809,403 + +0.6 4,809,404 - -0.3 4,809,404 - -1.0 4,809,455 + +0.6 4,809,456 - -1.4 4,809,488 - +0.6 4,809,538 + +0.6 4,809,546 + -0.8
Or see this region's nucleotide sequence