Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4342 and Pf1N1B4_4343 are separated by 27 nucleotides Pf1N1B4_4343 and Pf1N1B4_4344 overlap by 4 nucleotides Pf1N1B4_4344 and Pf1N1B4_4345 are separated by 317 nucleotides Pf1N1B4_4345 and Pf1N1B4_4346 are separated by 17 nucleotides Pf1N1B4_4346 and Pf1N1B4_4347 are separated by 14 nucleotides Pf1N1B4_4347 and Pf1N1B4_4348 are separated by 124 nucleotides
Pf1N1B4_4342 - hypothetical protein, at 4,801,385 to 4,801,528
_4342
Pf1N1B4_4343 - hypothetical protein, at 4,801,556 to 4,801,825
_4343
Pf1N1B4_4344 - hypothetical protein, at 4,801,822 to 4,801,977
_4344
Pf1N1B4_4345 - hypothetical protein, at 4,802,295 to 4,803,356
_4345
Pf1N1B4_4346 - Protein of unknown function DUF1428, at 4,803,374 to 4,803,727
_4346
Pf1N1B4_4347 - hypothetical protein, at 4,803,742 to 4,803,936
_4347
Pf1N1B4_4348 - 2'-5' RNA ligase, at 4,804,061 to 4,804,600
_4348
Position (kb)
4802
4803
4804 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4801.343 kb on + strand at 4801.347 kb on + strand at 4801.348 kb on - strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.359 kb on + strand at 4801.360 kb on - strand at 4801.360 kb on - strand at 4801.380 kb on + strand at 4801.396 kb on - strand at 4801.614 kb on - strand, within Pf1N1B4_4343 at 4801.770 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.773 kb on + strand, within Pf1N1B4_4343 at 4801.774 kb on - strand, within Pf1N1B4_4343 at 4801.787 kb on + strand, within Pf1N1B4_4343 at 4801.955 kb on + strand, within Pf1N1B4_4344 at 4802.015 kb on + strand at 4802.015 kb on + strand at 4802.016 kb on - strand at 4802.019 kb on + strand at 4802.059 kb on + strand at 4802.059 kb on + strand at 4802.060 kb on - strand at 4802.139 kb on + strand at 4802.139 kb on + strand at 4802.142 kb on - strand at 4802.226 kb on + strand at 4802.226 kb on + strand at 4802.226 kb on + strand at 4802.226 kb on + strand at 4802.227 kb on - strand at 4802.227 kb on - strand at 4802.227 kb on - strand at 4802.281 kb on - strand at 4802.480 kb on + strand, within Pf1N1B4_4345 at 4802.480 kb on + strand at 4802.480 kb on + strand, within Pf1N1B4_4345 at 4802.481 kb on - strand, within Pf1N1B4_4345 at 4802.655 kb on + strand, within Pf1N1B4_4345 at 4802.762 kb on + strand, within Pf1N1B4_4345 at 4803.008 kb on - strand, within Pf1N1B4_4345 at 4803.113 kb on + strand, within Pf1N1B4_4345 at 4803.237 kb on + strand, within Pf1N1B4_4345 at 4803.238 kb on - strand, within Pf1N1B4_4345 at 4803.238 kb on - strand, within Pf1N1B4_4345 at 4803.238 kb on - strand at 4803.380 kb on + strand at 4803.380 kb on + strand at 4803.381 kb on - strand at 4803.381 kb on - strand at 4803.405 kb on + strand at 4803.546 kb on + strand, within Pf1N1B4_4346 at 4803.546 kb on + strand, within Pf1N1B4_4346 at 4803.547 kb on - strand, within Pf1N1B4_4346 at 4803.843 kb on - strand at 4803.843 kb on - strand, within Pf1N1B4_4347 at 4803.843 kb on - strand, within Pf1N1B4_4347 at 4803.968 kb on + strand at 4803.968 kb on + strand at 4803.969 kb on - strand at 4803.969 kb on - strand at 4803.970 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,801,343 + +1.6 4,801,347 + -0.4 4,801,348 - -0.2 4,801,359 + +1.4 4,801,359 + -0.8 4,801,359 + -2.2 4,801,359 + +0.6 4,801,359 + -0.0 4,801,360 - +0.6 4,801,360 - -0.4 4,801,380 + -0.4 4,801,396 - +2.2 4,801,614 - Pf1N1B4_4343 0.21 +1.3 4,801,770 + Pf1N1B4_4343 0.79 -1.8 4,801,773 + Pf1N1B4_4343 0.80 -1.8 4,801,773 + Pf1N1B4_4343 0.80 -0.4 4,801,773 + +1.2 4,801,773 + Pf1N1B4_4343 0.80 -2.0 4,801,773 + Pf1N1B4_4343 0.80 -1.0 4,801,773 + Pf1N1B4_4343 0.80 +1.0 4,801,774 - Pf1N1B4_4343 0.81 -0.4 4,801,787 + Pf1N1B4_4343 0.86 +1.0 4,801,955 + Pf1N1B4_4344 0.85 +0.8 4,802,015 + -0.4 4,802,015 + -0.0 4,802,016 - +0.3 4,802,019 + -1.0 4,802,059 + +0.6 4,802,059 + -2.0 4,802,060 - +0.2 4,802,139 + +0.6 4,802,139 + +3.2 4,802,142 - -0.0 4,802,226 + -1.3 4,802,226 + -1.3 4,802,226 + +1.6 4,802,226 + -0.1 4,802,227 - +2.4 4,802,227 - -0.4 4,802,227 - -0.8 4,802,281 - +0.7 4,802,480 + Pf1N1B4_4345 0.17 +1.1 4,802,480 + -0.4 4,802,480 + Pf1N1B4_4345 0.17 -1.4 4,802,481 - Pf1N1B4_4345 0.18 +0.6 4,802,655 + Pf1N1B4_4345 0.34 -1.4 4,802,762 + Pf1N1B4_4345 0.44 +0.6 4,803,008 - Pf1N1B4_4345 0.67 +1.4 4,803,113 + Pf1N1B4_4345 0.77 -0.3 4,803,237 + Pf1N1B4_4345 0.89 +1.0 4,803,238 - Pf1N1B4_4345 0.89 -0.8 4,803,238 - Pf1N1B4_4345 0.89 +1.9 4,803,238 - -0.4 4,803,380 + +0.4 4,803,380 + +1.4 4,803,381 - +0.6 4,803,381 - +0.6 4,803,405 + +2.1 4,803,546 + Pf1N1B4_4346 0.49 -1.2 4,803,546 + Pf1N1B4_4346 0.49 -0.0 4,803,547 - Pf1N1B4_4346 0.49 +0.0 4,803,843 - +1.9 4,803,843 - Pf1N1B4_4347 0.52 +1.0 4,803,843 - Pf1N1B4_4347 0.52 +1.0 4,803,968 + +0.1 4,803,968 + -0.8 4,803,969 - +0.1 4,803,969 - +1.1 4,803,970 + +1.1
Or see this region's nucleotide sequence