Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4256 and Pf1N1B4_4257 are separated by 224 nucleotides Pf1N1B4_4257 and Pf1N1B4_4258 are separated by 65 nucleotides Pf1N1B4_4258 and Pf1N1B4_4259 are separated by 160 nucleotides
Pf1N1B4_4256 - Probable transmembrane protein, at 4,718,468 to 4,719,148
_4256
Pf1N1B4_4257 - L-serine dehydratase, (PLP)-dependent (EC 4.3.1.17) @ Threonine dehydratase biosynthetic (EC 4.3.1.19), at 4,719,373 to 4,720,305
_4257
Pf1N1B4_4258 - Ribonucleotide reductase, alpha subunit, at 4,720,371 to 4,720,610
_4258
Pf1N1B4_4259 - hypothetical protein, at 4,720,771 to 4,721,544
_4259
Position (kb)
4719
4720
4721 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4718.453 kb on + strand at 4718.453 kb on + strand at 4718.454 kb on - strand at 4718.454 kb on - strand at 4718.473 kb on - strand at 4718.497 kb on + strand at 4718.498 kb on - strand at 4718.517 kb on + strand at 4718.518 kb on - strand at 4718.518 kb on - strand at 4718.541 kb on - strand, within Pf1N1B4_4256 at 4718.664 kb on + strand at 4718.664 kb on + strand, within Pf1N1B4_4256 at 4718.861 kb on - strand, within Pf1N1B4_4256 at 4718.862 kb on + strand, within Pf1N1B4_4256 at 4718.863 kb on - strand, within Pf1N1B4_4256 at 4719.076 kb on - strand, within Pf1N1B4_4256 at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.225 kb on - strand at 4719.288 kb on + strand at 4719.288 kb on + strand at 4719.288 kb on + strand at 4719.289 kb on - strand at 4719.289 kb on - strand at 4719.289 kb on - strand at 4719.314 kb on + strand at 4719.314 kb on + strand at 4719.314 kb on + strand at 4719.314 kb on + strand at 4719.315 kb on - strand at 4719.315 kb on - strand at 4719.315 kb on - strand at 4719.745 kb on + strand, within Pf1N1B4_4257 at 4719.745 kb on + strand, within Pf1N1B4_4257 at 4719.745 kb on + strand, within Pf1N1B4_4257 at 4719.746 kb on - strand at 4719.746 kb on - strand, within Pf1N1B4_4257 at 4720.303 kb on + strand at 4720.317 kb on + strand at 4720.462 kb on + strand, within Pf1N1B4_4258 at 4720.500 kb on - strand, within Pf1N1B4_4258 at 4720.552 kb on + strand, within Pf1N1B4_4258 at 4720.600 kb on - strand at 4720.638 kb on + strand at 4720.647 kb on + strand at 4720.647 kb on + strand at 4720.648 kb on - strand at 4720.656 kb on + strand at 4720.657 kb on - strand at 4720.698 kb on - strand at 4720.706 kb on + strand at 4720.706 kb on + strand at 4720.707 kb on - strand at 4720.707 kb on - strand at 4720.707 kb on - strand at 4720.707 kb on - strand at 4720.754 kb on + strand at 4720.754 kb on + strand at 4721.174 kb on - strand, within Pf1N1B4_4259 at 4721.234 kb on - strand, within Pf1N1B4_4259
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,718,453 + -0.6 4,718,453 + -0.5 4,718,454 - -0.4 4,718,454 - -0.4 4,718,473 - -1.6 4,718,497 + +1.1 4,718,498 - -0.3 4,718,517 + -1.2 4,718,518 - +0.3 4,718,518 - -1.0 4,718,541 - Pf1N1B4_4256 0.11 -0.7 4,718,664 + -2.2 4,718,664 + Pf1N1B4_4256 0.29 +0.8 4,718,861 - Pf1N1B4_4256 0.58 -0.8 4,718,862 + Pf1N1B4_4256 0.58 -0.2 4,718,863 - Pf1N1B4_4256 0.58 -1.1 4,719,076 - Pf1N1B4_4256 0.89 +1.9 4,719,150 + +0.6 4,719,150 + -0.9 4,719,150 + -0.8 4,719,150 + +0.3 4,719,150 + -0.7 4,719,150 + -1.0 4,719,150 + -0.2 4,719,150 + +0.8 4,719,151 - +1.9 4,719,151 - +0.2 4,719,151 - -1.0 4,719,151 - +0.6 4,719,151 - -0.4 4,719,151 - +0.3 4,719,151 - -2.4 4,719,225 - -0.1 4,719,288 + +1.4 4,719,288 + -1.7 4,719,288 + -2.1 4,719,289 - +1.3 4,719,289 - -0.3 4,719,289 - +0.2 4,719,314 + +0.6 4,719,314 + -0.2 4,719,314 + -0.3 4,719,314 + +0.2 4,719,315 - +0.4 4,719,315 - +0.6 4,719,315 - +0.0 4,719,745 + Pf1N1B4_4257 0.40 -0.2 4,719,745 + Pf1N1B4_4257 0.40 +0.6 4,719,745 + Pf1N1B4_4257 0.40 -0.8 4,719,746 - +0.6 4,719,746 - Pf1N1B4_4257 0.40 -2.3 4,720,303 + -2.2 4,720,317 + +2.4 4,720,462 + Pf1N1B4_4258 0.38 +0.9 4,720,500 - Pf1N1B4_4258 0.54 -1.4 4,720,552 + Pf1N1B4_4258 0.75 +1.1 4,720,600 - -1.3 4,720,638 + +0.6 4,720,647 + +1.3 4,720,647 + +0.6 4,720,648 - -0.4 4,720,656 + +1.6 4,720,657 - -0.4 4,720,698 - +0.7 4,720,706 + +1.6 4,720,706 + +1.0 4,720,707 - -1.4 4,720,707 - +1.2 4,720,707 - +0.2 4,720,707 - +2.1 4,720,754 + +0.3 4,720,754 + -2.9 4,721,174 - Pf1N1B4_4259 0.52 +0.2 4,721,234 - Pf1N1B4_4259 0.60 +0.3
Or see this region's nucleotide sequence