Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4221 and Pf1N1B4_4222 are separated by 19 nucleotides Pf1N1B4_4222 and Pf1N1B4_4223 are separated by 6 nucleotides
Pf1N1B4_4221 - hypothetical protein, at 4,678,383 to 4,678,499
_4221
Pf1N1B4_4222 - putative tyrosinase, at 4,678,519 to 4,679,379
_4222
Pf1N1B4_4223 - Transcriptional regulator, at 4,679,386 to 4,680,279
_4223
Position (kb)
4678
4679
4680 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4677.553 kb on - strand at 4677.604 kb on + strand at 4677.671 kb on - strand at 4677.831 kb on + strand at 4677.831 kb on + strand at 4677.835 kb on + strand at 4677.836 kb on - strand at 4677.978 kb on + strand at 4677.979 kb on - strand at 4678.073 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.108 kb on + strand at 4678.109 kb on - strand at 4678.126 kb on + strand at 4678.269 kb on + strand at 4678.270 kb on - strand at 4678.362 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.498 kb on - strand at 4678.531 kb on + strand at 4678.604 kb on - strand at 4678.604 kb on - strand at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4679.011 kb on + strand, within Pf1N1B4_4222 at 4679.012 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.098 kb on - strand, within Pf1N1B4_4222 at 4679.230 kb on + strand, within Pf1N1B4_4222 at 4679.231 kb on - strand at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.335 kb on + strand at 4679.336 kb on - strand at 4679.345 kb on - strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.353 kb on + strand at 4679.377 kb on + strand at 4679.377 kb on + strand at 4679.501 kb on + strand, within Pf1N1B4_4223 at 4679.502 kb on - strand at 4679.734 kb on + strand, within Pf1N1B4_4223 at 4679.734 kb on + strand, within Pf1N1B4_4223 at 4679.807 kb on + strand, within Pf1N1B4_4223 at 4679.884 kb on - strand at 4679.933 kb on + strand at 4679.934 kb on - strand, within Pf1N1B4_4223 at 4679.934 kb on - strand, within Pf1N1B4_4223 at 4680.137 kb on - strand, within Pf1N1B4_4223 at 4680.244 kb on - strand at 4680.303 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,677,553 - +1.4 4,677,604 + +0.0 4,677,671 - +0.3 4,677,831 + +2.2 4,677,831 + -0.4 4,677,835 + -1.7 4,677,836 - +0.1 4,677,978 + +0.0 4,677,979 - -0.4 4,678,073 + -1.4 4,678,108 + -2.2 4,678,108 + +1.2 4,678,108 + -0.2 4,678,108 + +0.2 4,678,108 + +0.3 4,678,108 + +0.6 4,678,109 - -2.2 4,678,126 + -1.2 4,678,269 + +0.8 4,678,270 - +0.6 4,678,362 + -1.2 4,678,497 + -0.1 4,678,497 + +0.3 4,678,497 + +0.5 4,678,497 + +0.8 4,678,498 - +0.2 4,678,531 + +0.5 4,678,604 - -0.4 4,678,604 - -1.4 4,678,846 + Pf1N1B4_4222 0.38 +0.6 4,678,846 + Pf1N1B4_4222 0.38 +0.0 4,679,011 + Pf1N1B4_4222 0.57 -0.4 4,679,012 - Pf1N1B4_4222 0.57 -1.1 4,679,027 - Pf1N1B4_4222 0.59 -0.3 4,679,027 - Pf1N1B4_4222 0.59 -0.8 4,679,027 - Pf1N1B4_4222 0.59 -1.0 4,679,098 - Pf1N1B4_4222 0.67 -0.4 4,679,230 + Pf1N1B4_4222 0.83 -1.2 4,679,231 - -0.4 4,679,234 + Pf1N1B4_4222 0.83 +0.6 4,679,234 + Pf1N1B4_4222 0.83 -1.2 4,679,235 - Pf1N1B4_4222 0.83 -0.4 4,679,235 - Pf1N1B4_4222 0.83 -0.2 4,679,290 + Pf1N1B4_4222 0.90 -0.7 4,679,290 + Pf1N1B4_4222 0.90 -1.5 4,679,291 - Pf1N1B4_4222 0.90 -0.4 4,679,291 - Pf1N1B4_4222 0.90 -0.4 4,679,291 - Pf1N1B4_4222 0.90 -2.2 4,679,335 + -1.0 4,679,336 - -1.4 4,679,345 - -1.4 4,679,347 + +0.5 4,679,347 + +0.6 4,679,347 + +0.6 4,679,347 + +1.4 4,679,348 - +0.6 4,679,348 - +1.2 4,679,348 - -1.1 4,679,353 + +1.0 4,679,377 + +0.0 4,679,377 + -1.0 4,679,501 + Pf1N1B4_4223 0.13 -0.1 4,679,502 - +2.2 4,679,734 + Pf1N1B4_4223 0.39 -0.7 4,679,734 + Pf1N1B4_4223 0.39 +0.6 4,679,807 + Pf1N1B4_4223 0.47 +0.6 4,679,884 - +1.6 4,679,933 + +0.6 4,679,934 - Pf1N1B4_4223 0.61 -2.5 4,679,934 - Pf1N1B4_4223 0.61 +0.2 4,680,137 - Pf1N1B4_4223 0.84 +1.1 4,680,244 - +1.2 4,680,303 + +1.6
Or see this region's nucleotide sequence