Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4199 and Pf1N1B4_4200 are separated by 126 nucleotides Pf1N1B4_4200 and Pf1N1B4_4201 overlap by 26 nucleotides
Pf1N1B4_4199 - Protocatechuate 4,5-dioxygenase beta chain (EC 1.13.11.8), at 4,650,399 to 4,651,661
_4199
Pf1N1B4_4200 - PhaK-like protein, at 4,651,788 to 4,653,035
_4200
Pf1N1B4_4201 - Transcriptional regulator, LysR family, at 4,653,010 to 4,654,245
_4201
Position (kb)
4651
4652
4653
4654 Strain fitness (log2 ratio)
-1
0
1 at 4651.183 kb on - strand, within Pf1N1B4_4199 at 4651.183 kb on - strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.185 kb on + strand, within Pf1N1B4_4199 at 4651.503 kb on + strand, within Pf1N1B4_4199 at 4651.504 kb on - strand, within Pf1N1B4_4199 at 4651.569 kb on + strand at 4651.783 kb on + strand at 4651.784 kb on - strand at 4651.912 kb on - strand at 4652.215 kb on + strand, within Pf1N1B4_4200 at 4652.215 kb on + strand, within Pf1N1B4_4200 at 4652.216 kb on - strand at 4652.298 kb on + strand, within Pf1N1B4_4200 at 4652.484 kb on + strand, within Pf1N1B4_4200 at 4652.485 kb on - strand, within Pf1N1B4_4200 at 4652.485 kb on - strand, within Pf1N1B4_4200 at 4652.636 kb on + strand, within Pf1N1B4_4200 at 4652.643 kb on + strand at 4652.727 kb on + strand, within Pf1N1B4_4200 at 4652.728 kb on - strand, within Pf1N1B4_4200 at 4652.728 kb on - strand at 4652.740 kb on - strand, within Pf1N1B4_4200 at 4652.829 kb on + strand, within Pf1N1B4_4200 at 4653.166 kb on + strand at 4653.485 kb on + strand, within Pf1N1B4_4201 at 4653.486 kb on - strand, within Pf1N1B4_4201 at 4653.486 kb on - strand, within Pf1N1B4_4201 at 4653.585 kb on + strand, within Pf1N1B4_4201 at 4653.586 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,651,183 - Pf1N1B4_4199 0.62 -1.3 4,651,183 - Pf1N1B4_4199 0.62 +1.5 4,651,185 + Pf1N1B4_4199 0.62 -0.3 4,651,185 + Pf1N1B4_4199 0.62 -1.1 4,651,185 + Pf1N1B4_4199 0.62 -0.2 4,651,503 + Pf1N1B4_4199 0.87 +1.0 4,651,504 - Pf1N1B4_4199 0.87 +0.6 4,651,569 + -1.3 4,651,783 + -1.4 4,651,784 - -0.4 4,651,912 - +0.2 4,652,215 + Pf1N1B4_4200 0.34 -0.2 4,652,215 + Pf1N1B4_4200 0.34 -0.4 4,652,216 - +1.6 4,652,298 + Pf1N1B4_4200 0.41 -0.7 4,652,484 + Pf1N1B4_4200 0.56 +1.0 4,652,485 - Pf1N1B4_4200 0.56 +0.6 4,652,485 - Pf1N1B4_4200 0.56 +1.0 4,652,636 + Pf1N1B4_4200 0.68 +1.7 4,652,643 + +1.6 4,652,727 + Pf1N1B4_4200 0.75 -0.3 4,652,728 - Pf1N1B4_4200 0.75 +0.9 4,652,728 - -0.4 4,652,740 - Pf1N1B4_4200 0.76 -0.3 4,652,829 + Pf1N1B4_4200 0.83 +1.6 4,653,166 + +0.6 4,653,485 + Pf1N1B4_4201 0.38 -0.4 4,653,486 - Pf1N1B4_4201 0.39 -0.1 4,653,486 - Pf1N1B4_4201 0.39 +1.2 4,653,585 + Pf1N1B4_4201 0.47 -0.1 4,653,586 - -0.4
Or see this region's nucleotide sequence