Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4158 and Pf1N1B4_4159 overlap by 1 nucleotides Pf1N1B4_4159 and Pf1N1B4_4160 overlap by 4 nucleotides Pf1N1B4_4160 and Pf1N1B4_4161 overlap by 4 nucleotides
Pf1N1B4_4158 - FIG00955149: hypothetical protein, at 4,602,890 to 4,604,377
_4158
Pf1N1B4_4159 - FIG00955149: hypothetical protein, at 4,604,377 to 4,604,604
_4159
Pf1N1B4_4160 - FIG00956113: hypothetical protein, at 4,604,601 to 4,605,239
_4160
Pf1N1B4_4161 - UPF0192 protein all5100 precursor, at 4,605,236 to 4,609,810
_4161
Position (kb)
4604
4605 Strain fitness (log2 ratio)
-1
0
1 at 4603.406 kb on - strand, within Pf1N1B4_4158 at 4603.472 kb on + strand, within Pf1N1B4_4158 at 4603.473 kb on - strand, within Pf1N1B4_4158 at 4603.473 kb on - strand, within Pf1N1B4_4158 at 4604.003 kb on + strand, within Pf1N1B4_4158 at 4604.029 kb on - strand, within Pf1N1B4_4158 at 4604.029 kb on - strand, within Pf1N1B4_4158 at 4604.244 kb on + strand at 4604.386 kb on + strand at 4604.387 kb on - strand at 4604.468 kb on - strand, within Pf1N1B4_4159 at 4604.468 kb on - strand, within Pf1N1B4_4159 at 4604.486 kb on - strand, within Pf1N1B4_4159 at 4604.527 kb on + strand, within Pf1N1B4_4159 at 4604.528 kb on - strand, within Pf1N1B4_4159 at 4604.528 kb on - strand, within Pf1N1B4_4159 at 4604.950 kb on - strand, within Pf1N1B4_4160 at 4605.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,603,406 - Pf1N1B4_4158 0.35 -1.0 4,603,472 + Pf1N1B4_4158 0.39 +0.7 4,603,473 - Pf1N1B4_4158 0.39 -0.6 4,603,473 - Pf1N1B4_4158 0.39 +0.4 4,604,003 + Pf1N1B4_4158 0.75 -1.4 4,604,029 - Pf1N1B4_4158 0.77 +0.9 4,604,029 - Pf1N1B4_4158 0.77 +0.4 4,604,244 + +0.0 4,604,386 + -0.6 4,604,387 - +0.2 4,604,468 - Pf1N1B4_4159 0.40 +1.1 4,604,468 - Pf1N1B4_4159 0.40 +0.4 4,604,486 - Pf1N1B4_4159 0.48 +0.9 4,604,527 + Pf1N1B4_4159 0.66 +0.0 4,604,528 - Pf1N1B4_4159 0.66 +0.0 4,604,528 - Pf1N1B4_4159 0.66 +0.9 4,604,950 - Pf1N1B4_4160 0.55 +1.2 4,605,468 - -0.3
Or see this region's nucleotide sequence