Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3984 and Pf1N1B4_3985 overlap by 1 nucleotides Pf1N1B4_3985 and Pf1N1B4_3986 are separated by 115 nucleotides
Pf1N1B4_3984 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 4,424,874 to 4,426,829
_3984
Pf1N1B4_3985 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 4,426,829 to 4,427,644
_3985
Pf1N1B4_3986 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 4,427,760 to 4,429,367
_3986
Position (kb)
4426
4427
4428 Strain fitness (log2 ratio)
-2
-1
0
1 at 4426.015 kb on + strand, within Pf1N1B4_3984 at 4426.150 kb on + strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.152 kb on + strand, within Pf1N1B4_3984 at 4426.153 kb on - strand, within Pf1N1B4_3984 at 4426.153 kb on - strand, within Pf1N1B4_3984 at 4426.213 kb on + strand, within Pf1N1B4_3984 at 4426.214 kb on - strand, within Pf1N1B4_3984 at 4426.639 kb on + strand at 4426.640 kb on - strand at 4426.640 kb on - strand at 4427.321 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.891 kb on - strand at 4427.891 kb on - strand at 4427.891 kb on - strand at 4427.891 kb on - strand at 4427.894 kb on - strand at 4428.104 kb on - strand, within Pf1N1B4_3986 at 4428.215 kb on - strand, within Pf1N1B4_3986 at 4428.392 kb on + strand, within Pf1N1B4_3986
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,426,015 + Pf1N1B4_3984 0.58 +0.1 4,426,150 + Pf1N1B4_3984 0.65 -1.6 4,426,151 - Pf1N1B4_3984 0.65 -0.3 4,426,151 - Pf1N1B4_3984 0.65 +0.7 4,426,151 - Pf1N1B4_3984 0.65 +0.4 4,426,151 - Pf1N1B4_3984 0.65 +1.7 4,426,151 - Pf1N1B4_3984 0.65 +1.1 4,426,152 + Pf1N1B4_3984 0.65 -0.2 4,426,153 - Pf1N1B4_3984 0.65 -0.9 4,426,153 - Pf1N1B4_3984 0.65 -0.9 4,426,213 + Pf1N1B4_3984 0.68 +0.2 4,426,214 - Pf1N1B4_3984 0.69 +1.7 4,426,639 + -0.3 4,426,640 - +1.4 4,426,640 - -2.3 4,427,321 + Pf1N1B4_3985 0.60 -2.1 4,427,361 + Pf1N1B4_3985 0.65 -0.6 4,427,361 + Pf1N1B4_3985 0.65 -0.6 4,427,361 + Pf1N1B4_3985 0.65 -0.3 4,427,891 - -0.7 4,427,891 - -0.1 4,427,891 - +0.2 4,427,891 - -0.1 4,427,894 - -0.3 4,428,104 - Pf1N1B4_3986 0.21 +0.3 4,428,215 - Pf1N1B4_3986 0.28 -2.1 4,428,392 + Pf1N1B4_3986 0.39 +0.0
Or see this region's nucleotide sequence