Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3967 and Pf1N1B4_3968 are separated by 228 nucleotides Pf1N1B4_3968 and Pf1N1B4_3969 are separated by 17 nucleotides
Pf1N1B4_3967 - AFG1-like ATPase, at 4,408,021 to 4,409,151
_3967
Pf1N1B4_3968 - Spermidine/putrescine-binding periplasmic protein, at 4,409,380 to 4,410,423
_3968
Pf1N1B4_3969 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 4,410,441 to 4,411,556
_3969
Position (kb)
4409
4410
4411 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4408.436 kb on + strand, within Pf1N1B4_3967 at 4408.895 kb on + strand at 4408.895 kb on + strand, within Pf1N1B4_3967 at 4408.895 kb on + strand, within Pf1N1B4_3967 at 4408.896 kb on - strand, within Pf1N1B4_3967 at 4408.896 kb on - strand, within Pf1N1B4_3967 at 4408.997 kb on + strand, within Pf1N1B4_3967 at 4409.027 kb on + strand, within Pf1N1B4_3967 at 4409.027 kb on + strand, within Pf1N1B4_3967 at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.118 kb on + strand at 4409.119 kb on - strand at 4409.173 kb on + strand at 4409.201 kb on - strand at 4409.201 kb on - strand at 4409.201 kb on - strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.247 kb on + strand at 4409.248 kb on - strand at 4409.515 kb on - strand, within Pf1N1B4_3968 at 4409.685 kb on - strand, within Pf1N1B4_3968 at 4409.798 kb on + strand, within Pf1N1B4_3968 at 4409.799 kb on - strand, within Pf1N1B4_3968 at 4410.023 kb on + strand, within Pf1N1B4_3968 at 4410.023 kb on + strand, within Pf1N1B4_3968 at 4410.024 kb on - strand, within Pf1N1B4_3968 at 4410.150 kb on - strand, within Pf1N1B4_3968 at 4410.336 kb on - strand at 4410.640 kb on + strand, within Pf1N1B4_3969 at 4410.641 kb on - strand, within Pf1N1B4_3969 at 4410.820 kb on + strand, within Pf1N1B4_3969 at 4410.820 kb on + strand, within Pf1N1B4_3969 at 4410.820 kb on + strand, within Pf1N1B4_3969 at 4410.821 kb on - strand at 4410.821 kb on - strand, within Pf1N1B4_3969 at 4410.821 kb on - strand, within Pf1N1B4_3969 at 4410.821 kb on - strand, within Pf1N1B4_3969 at 4410.821 kb on - strand, within Pf1N1B4_3969 at 4410.821 kb on - strand, within Pf1N1B4_3969 at 4410.821 kb on - strand, within Pf1N1B4_3969 at 4411.249 kb on + strand, within Pf1N1B4_3969 at 4411.420 kb on + strand, within Pf1N1B4_3969 at 4411.420 kb on + strand, within Pf1N1B4_3969
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,408,436 + Pf1N1B4_3967 0.37 -0.8 4,408,895 + +1.3 4,408,895 + Pf1N1B4_3967 0.77 +2.7 4,408,895 + Pf1N1B4_3967 0.77 +0.5 4,408,896 - Pf1N1B4_3967 0.77 -1.0 4,408,896 - Pf1N1B4_3967 0.77 +0.2 4,408,997 + Pf1N1B4_3967 0.86 -0.3 4,409,027 + Pf1N1B4_3967 0.89 -0.1 4,409,027 + Pf1N1B4_3967 0.89 -0.1 4,409,118 + -2.3 4,409,118 + +1.1 4,409,118 + -0.8 4,409,118 + +1.5 4,409,118 + -1.9 4,409,118 + +1.5 4,409,119 - -0.0 4,409,173 + -0.2 4,409,201 - -0.5 4,409,201 - -0.3 4,409,201 - -0.3 4,409,247 + +0.4 4,409,247 + +0.7 4,409,247 + +2.0 4,409,247 + -0.4 4,409,247 + -0.2 4,409,247 + +1.4 4,409,248 - +0.3 4,409,515 - Pf1N1B4_3968 0.13 +2.5 4,409,685 - Pf1N1B4_3968 0.29 +0.7 4,409,798 + Pf1N1B4_3968 0.40 +0.1 4,409,799 - Pf1N1B4_3968 0.40 +1.1 4,410,023 + Pf1N1B4_3968 0.62 +2.7 4,410,023 + Pf1N1B4_3968 0.62 +1.1 4,410,024 - Pf1N1B4_3968 0.62 -1.3 4,410,150 - Pf1N1B4_3968 0.74 +0.3 4,410,336 - -0.9 4,410,640 + Pf1N1B4_3969 0.18 +0.7 4,410,641 - Pf1N1B4_3969 0.18 -0.3 4,410,820 + Pf1N1B4_3969 0.34 -0.3 4,410,820 + Pf1N1B4_3969 0.34 -0.3 4,410,820 + Pf1N1B4_3969 0.34 +0.7 4,410,821 - -0.3 4,410,821 - Pf1N1B4_3969 0.34 +1.0 4,410,821 - Pf1N1B4_3969 0.34 -0.1 4,410,821 - Pf1N1B4_3969 0.34 +0.7 4,410,821 - Pf1N1B4_3969 0.34 +0.1 4,410,821 - Pf1N1B4_3969 0.34 -1.3 4,410,821 - Pf1N1B4_3969 0.34 -0.6 4,411,249 + Pf1N1B4_3969 0.72 -0.2 4,411,420 + Pf1N1B4_3969 0.88 +1.2 4,411,420 + Pf1N1B4_3969 0.88 -1.5
Or see this region's nucleotide sequence