Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_386 and Pf1N1B4_387 overlap by 24 nucleotides Pf1N1B4_387 and Pf1N1B4_388 are separated by 120 nucleotides Pf1N1B4_388 and Pf1N1B4_389 overlap by 4 nucleotides Pf1N1B4_389 and Pf1N1B4_390 are separated by 9 nucleotides
Pf1N1B4_386 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 455,166 to 456,605
_386
Pf1N1B4_387 - hypothetical protein, at 456,582 to 456,695
_387
Pf1N1B4_388 - OmpA family protein, at 456,816 to 457,628
_388
Pf1N1B4_389 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 457,625 to 457,981
_389
Pf1N1B4_390 - Extracellular solute-binding protein, family 3, at 457,991 to 458,827
_390
Position (kb) 456
457
458 Strain fitness (log2 ratio) -2
-1
0
1
2 at 455.844 kb on - strand, within Pf1N1B4_386 at 455.844 kb on - strand, within Pf1N1B4_386 at 455.989 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 455.992 kb on + strand, within Pf1N1B4_386 at 456.036 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.172 kb on + strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.173 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.245 kb on - strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.403 kb on + strand, within Pf1N1B4_386 at 456.404 kb on - strand, within Pf1N1B4_386 at 456.404 kb on - strand, within Pf1N1B4_386 at 456.538 kb on + strand at 456.538 kb on + strand at 456.539 kb on - strand at 456.539 kb on - strand at 456.578 kb on + strand at 456.578 kb on + strand at 456.578 kb on + strand at 456.578 kb on + strand at 456.579 kb on - strand at 456.579 kb on - strand at 456.579 kb on - strand at 456.579 kb on - strand at 456.586 kb on + strand at 456.586 kb on + strand at 456.586 kb on + strand at 456.586 kb on + strand at 456.634 kb on + strand, within Pf1N1B4_387 at 456.634 kb on + strand, within Pf1N1B4_387 at 456.634 kb on + strand, within Pf1N1B4_387 at 456.635 kb on - strand, within Pf1N1B4_387 at 456.665 kb on + strand, within Pf1N1B4_387 at 456.673 kb on + strand, within Pf1N1B4_387 at 456.703 kb on + strand at 456.703 kb on + strand at 456.703 kb on + strand at 456.703 kb on + strand at 456.944 kb on + strand at 456.944 kb on + strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 456.945 kb on - strand, within Pf1N1B4_388 at 457.048 kb on + strand, within Pf1N1B4_388 at 457.049 kb on - strand, within Pf1N1B4_388 at 457.049 kb on - strand, within Pf1N1B4_388 at 457.366 kb on + strand, within Pf1N1B4_388 at 457.367 kb on - strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.381 kb on + strand, within Pf1N1B4_388 at 457.382 kb on - strand, within Pf1N1B4_388 at 457.387 kb on + strand, within Pf1N1B4_388 at 457.388 kb on - strand, within Pf1N1B4_388 at 457.388 kb on - strand, within Pf1N1B4_388 at 457.418 kb on - strand, within Pf1N1B4_388 at 457.418 kb on - strand, within Pf1N1B4_388 at 457.571 kb on - strand at 457.670 kb on - strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.898 kb on + strand, within Pf1N1B4_389 at 457.899 kb on - strand, within Pf1N1B4_389 at 457.899 kb on - strand, within Pf1N1B4_389 at 457.899 kb on - strand, within Pf1N1B4_389 at 457.899 kb on - strand, within Pf1N1B4_389 at 457.899 kb on - strand, within Pf1N1B4_389 at 457.899 kb on - strand, within Pf1N1B4_389 at 457.899 kb on - strand, within Pf1N1B4_389 at 457.975 kb on + strand at 457.975 kb on + strand at 457.976 kb on - strand at 457.976 kb on - strand at 457.982 kb on + strand at 457.983 kb on - strand at 457.992 kb on + strand at 458.113 kb on + strand, within Pf1N1B4_390 at 458.155 kb on + strand, within Pf1N1B4_390 at 458.257 kb on - strand, within Pf1N1B4_390 at 458.375 kb on + strand, within Pf1N1B4_390 at 458.375 kb on + strand, within Pf1N1B4_390 at 458.433 kb on + strand, within Pf1N1B4_390 at 458.441 kb on + strand, within Pf1N1B4_390 at 458.469 kb on + strand, within Pf1N1B4_390 at 458.499 kb on + strand, within Pf1N1B4_390 at 458.499 kb on + strand at 458.499 kb on + strand, within Pf1N1B4_390 at 458.499 kb on + strand, within Pf1N1B4_390 at 458.500 kb on - strand, within Pf1N1B4_390 at 458.500 kb on - strand, within Pf1N1B4_390 at 458.601 kb on + strand, within Pf1N1B4_390
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 455,844 - Pf1N1B4_386 0.47 +1.9 455,844 - Pf1N1B4_386 0.47 +0.9 455,989 + Pf1N1B4_386 0.57 +0.9 455,992 + Pf1N1B4_386 0.57 +0.5 455,992 + Pf1N1B4_386 0.57 -1.5 455,992 + Pf1N1B4_386 0.57 +1.3 455,992 + +0.9 455,992 + Pf1N1B4_386 0.57 -0.1 455,992 + Pf1N1B4_386 0.57 -0.1 455,992 + Pf1N1B4_386 0.57 -0.1 455,992 + Pf1N1B4_386 0.57 -0.9 455,992 + Pf1N1B4_386 0.57 -2.5 455,992 + Pf1N1B4_386 0.57 -0.1 456,036 + Pf1N1B4_386 0.60 +0.2 456,172 + Pf1N1B4_386 0.70 +0.1 456,172 + Pf1N1B4_386 0.70 +2.5 456,172 + Pf1N1B4_386 0.70 +0.5 456,172 + +0.5 456,172 + Pf1N1B4_386 0.70 +0.1 456,172 + Pf1N1B4_386 0.70 -1.3 456,172 + Pf1N1B4_386 0.70 -1.0 456,173 - Pf1N1B4_386 0.70 +0.0 456,173 - Pf1N1B4_386 0.70 +0.6 456,173 - Pf1N1B4_386 0.70 -0.9 456,173 - Pf1N1B4_386 0.70 -2.5 456,173 - Pf1N1B4_386 0.70 -0.3 456,245 - Pf1N1B4_386 0.75 +0.0 456,245 - Pf1N1B4_386 0.75 -0.1 456,245 - Pf1N1B4_386 0.75 +1.2 456,245 - Pf1N1B4_386 0.75 -0.3 456,403 + Pf1N1B4_386 0.86 -1.7 456,403 + Pf1N1B4_386 0.86 -0.5 456,403 + Pf1N1B4_386 0.86 -0.4 456,403 + Pf1N1B4_386 0.86 -1.5 456,403 + Pf1N1B4_386 0.86 +0.9 456,403 + Pf1N1B4_386 0.86 -0.2 456,404 - Pf1N1B4_386 0.86 -1.7 456,404 - Pf1N1B4_386 0.86 +1.4 456,538 + -0.7 456,538 + -0.2 456,539 - -1.1 456,539 - +0.1 456,578 + -0.7 456,578 + +0.4 456,578 + -0.1 456,578 + +0.1 456,579 - -0.9 456,579 - +0.5 456,579 - -1.1 456,579 - -0.1 456,586 + -0.1 456,586 + -1.2 456,586 + +0.3 456,586 + +0.9 456,634 + Pf1N1B4_387 0.46 -1.1 456,634 + Pf1N1B4_387 0.46 +0.9 456,634 + Pf1N1B4_387 0.46 -2.1 456,635 - Pf1N1B4_387 0.46 +2.7 456,665 + Pf1N1B4_387 0.73 +0.1 456,673 + Pf1N1B4_387 0.80 -0.4 456,703 + -0.5 456,703 + +1.1 456,703 + +0.9 456,703 + +0.9 456,944 + -0.1 456,944 + Pf1N1B4_388 0.16 -1.1 456,945 - Pf1N1B4_388 0.16 -1.5 456,945 - Pf1N1B4_388 0.16 -1.5 456,945 - Pf1N1B4_388 0.16 -0.5 456,945 - Pf1N1B4_388 0.16 -1.3 456,945 - -0.1 456,945 - Pf1N1B4_388 0.16 +0.6 456,945 - Pf1N1B4_388 0.16 -2.2 456,945 - Pf1N1B4_388 0.16 -1.5 457,048 + Pf1N1B4_388 0.29 -0.5 457,049 - Pf1N1B4_388 0.29 -2.0 457,049 - Pf1N1B4_388 0.29 -1.8 457,366 + Pf1N1B4_388 0.68 -1.9 457,367 - Pf1N1B4_388 0.68 -2.7 457,381 + Pf1N1B4_388 0.69 -2.7 457,381 + Pf1N1B4_388 0.69 -1.5 457,381 + Pf1N1B4_388 0.69 +0.9 457,381 + Pf1N1B4_388 0.69 +1.5 457,381 + Pf1N1B4_388 0.69 -0.5 457,382 - Pf1N1B4_388 0.70 +0.9 457,387 + Pf1N1B4_388 0.70 -1.3 457,388 - Pf1N1B4_388 0.70 +0.9 457,388 - Pf1N1B4_388 0.70 -1.9 457,418 - Pf1N1B4_388 0.74 -1.5 457,418 - Pf1N1B4_388 0.74 +0.1 457,571 - -1.1 457,670 - Pf1N1B4_389 0.13 -0.7 457,898 + Pf1N1B4_389 0.76 +0.5 457,898 + Pf1N1B4_389 0.76 -0.5 457,898 + Pf1N1B4_389 0.76 -1.4 457,898 + Pf1N1B4_389 0.76 -2.1 457,898 + Pf1N1B4_389 0.76 -0.2 457,898 + Pf1N1B4_389 0.76 -1.3 457,898 + Pf1N1B4_389 0.76 +0.5 457,898 + Pf1N1B4_389 0.76 -1.7 457,898 + Pf1N1B4_389 0.76 -1.5 457,898 + Pf1N1B4_389 0.76 -1.0 457,898 + Pf1N1B4_389 0.76 -1.3 457,898 + Pf1N1B4_389 0.76 -0.9 457,898 + Pf1N1B4_389 0.76 -1.1 457,898 + Pf1N1B4_389 0.76 +0.3 457,898 + Pf1N1B4_389 0.76 -2.7 457,899 - Pf1N1B4_389 0.77 -1.1 457,899 - Pf1N1B4_389 0.77 -2.3 457,899 - Pf1N1B4_389 0.77 +0.3 457,899 - Pf1N1B4_389 0.77 -2.4 457,899 - Pf1N1B4_389 0.77 -0.1 457,899 - Pf1N1B4_389 0.77 -2.7 457,899 - Pf1N1B4_389 0.77 -1.5 457,975 + -1.5 457,975 + -1.6 457,976 - -1.7 457,976 - -1.4 457,982 + -1.3 457,983 - -0.3 457,992 + -1.9 458,113 + Pf1N1B4_390 0.15 -2.1 458,155 + Pf1N1B4_390 0.20 +0.7 458,257 - Pf1N1B4_390 0.32 -1.3 458,375 + Pf1N1B4_390 0.46 -1.3 458,375 + Pf1N1B4_390 0.46 -0.4 458,433 + Pf1N1B4_390 0.53 -0.5 458,441 + Pf1N1B4_390 0.54 +0.3 458,469 + Pf1N1B4_390 0.57 +1.0 458,499 + Pf1N1B4_390 0.61 -0.3 458,499 + +2.5 458,499 + Pf1N1B4_390 0.61 +1.5 458,499 + Pf1N1B4_390 0.61 +0.6 458,500 - Pf1N1B4_390 0.61 +0.4 458,500 - Pf1N1B4_390 0.61 -0.7 458,601 + Pf1N1B4_390 0.73 +0.3
Or see this region's nucleotide sequence