Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3829 and Pf1N1B4_3830 are separated by 63 nucleotides Pf1N1B4_3830 and Pf1N1B4_3831 are separated by 2 nucleotides Pf1N1B4_3831 and Pf1N1B4_3832 are separated by 290 nucleotides Pf1N1B4_3832 and Pf1N1B4_3833 are separated by 107 nucleotides
Pf1N1B4_3829 - Magnesium and cobalt transport protein CorA, at 4,243,578 to 4,244,573
_3829
Pf1N1B4_3830 - CrfX protein, at 4,244,637 to 4,244,885
_3830
Pf1N1B4_3831 - CmpX, at 4,244,888 to 4,245,712
_3831
Pf1N1B4_3832 - DNA-directed RNA polymerase specialized sigma subunit, sigma24-like, at 4,246,003 to 4,246,389
_3832
Pf1N1B4_3833 - Major porin and structural outer membrane porin OprF, at 4,246,497 to 4,247,531
_3833
Position (kb)
4244
4245
4246 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.030 kb on + strand, within Pf1N1B4_3829 at 4244.030 kb on + strand, within Pf1N1B4_3829 at 4244.030 kb on + strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.040 kb on + strand, within Pf1N1B4_3829 at 4244.040 kb on + strand, within Pf1N1B4_3829 at 4244.040 kb on + strand, within Pf1N1B4_3829 at 4244.041 kb on - strand at 4244.041 kb on - strand, within Pf1N1B4_3829 at 4244.041 kb on - strand, within Pf1N1B4_3829 at 4244.041 kb on - strand, within Pf1N1B4_3829 at 4244.049 kb on - strand, within Pf1N1B4_3829 at 4244.129 kb on + strand, within Pf1N1B4_3829 at 4244.129 kb on + strand, within Pf1N1B4_3829 at 4244.129 kb on + strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.207 kb on + strand, within Pf1N1B4_3829 at 4244.208 kb on - strand, within Pf1N1B4_3829 at 4244.230 kb on + strand at 4244.230 kb on + strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.237 kb on - strand, within Pf1N1B4_3829 at 4244.392 kb on - strand, within Pf1N1B4_3829 at 4244.392 kb on - strand, within Pf1N1B4_3829 at 4244.392 kb on - strand, within Pf1N1B4_3829 at 4244.392 kb on - strand, within Pf1N1B4_3829 at 4244.469 kb on - strand, within Pf1N1B4_3829 at 4244.549 kb on - strand at 4244.549 kb on - strand at 4244.620 kb on + strand at 4244.620 kb on + strand at 4244.620 kb on + strand at 4244.621 kb on - strand at 4244.621 kb on - strand at 4244.621 kb on - strand at 4244.621 kb on - strand at 4244.621 kb on - strand at 4244.621 kb on - strand at 4244.621 kb on - strand at 4244.636 kb on + strand at 4244.636 kb on + strand at 4244.636 kb on + strand at 4244.636 kb on + strand at 4244.636 kb on + strand at 4244.637 kb on - strand at 4244.637 kb on - strand at 4244.825 kb on + strand, within Pf1N1B4_3830 at 4244.825 kb on + strand, within Pf1N1B4_3830 at 4244.849 kb on + strand, within Pf1N1B4_3830 at 4244.850 kb on - strand, within Pf1N1B4_3830 at 4244.850 kb on - strand, within Pf1N1B4_3830 at 4244.850 kb on - strand, within Pf1N1B4_3830 at 4244.855 kb on + strand, within Pf1N1B4_3830 at 4244.855 kb on + strand, within Pf1N1B4_3830 at 4244.873 kb on + strand at 4244.873 kb on + strand at 4244.874 kb on - strand at 4245.124 kb on + strand, within Pf1N1B4_3831 at 4245.143 kb on - strand, within Pf1N1B4_3831 at 4245.171 kb on + strand, within Pf1N1B4_3831 at 4245.171 kb on + strand, within Pf1N1B4_3831 at 4245.171 kb on + strand, within Pf1N1B4_3831 at 4245.172 kb on - strand, within Pf1N1B4_3831 at 4245.242 kb on + strand, within Pf1N1B4_3831 at 4245.242 kb on + strand, within Pf1N1B4_3831 at 4245.242 kb on + strand, within Pf1N1B4_3831 at 4245.242 kb on + strand, within Pf1N1B4_3831 at 4245.242 kb on + strand, within Pf1N1B4_3831 at 4245.243 kb on - strand, within Pf1N1B4_3831 at 4245.256 kb on - strand, within Pf1N1B4_3831 at 4245.400 kb on + strand, within Pf1N1B4_3831 at 4245.400 kb on + strand, within Pf1N1B4_3831 at 4245.400 kb on + strand, within Pf1N1B4_3831 at 4245.400 kb on + strand, within Pf1N1B4_3831 at 4245.401 kb on - strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand, within Pf1N1B4_3831 at 4245.548 kb on + strand at 4245.549 kb on - strand, within Pf1N1B4_3831 at 4245.549 kb on - strand, within Pf1N1B4_3831 at 4245.563 kb on + strand, within Pf1N1B4_3831 at 4245.563 kb on + strand at 4245.563 kb on + strand, within Pf1N1B4_3831 at 4245.563 kb on + strand, within Pf1N1B4_3831 at 4245.564 kb on - strand, within Pf1N1B4_3831 at 4245.564 kb on - strand at 4245.728 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.733 kb on + strand at 4245.804 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.387 kb on + strand at 4246.401 kb on + strand at 4246.401 kb on + strand at 4246.401 kb on + strand at 4246.401 kb on + strand at 4246.402 kb on - strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.408 kb on + strand at 4246.409 kb on - strand at 4246.414 kb on + strand at 4246.414 kb on + strand at 4246.674 kb on + strand, within Pf1N1B4_3833
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,244,028 + Pf1N1B4_3829 0.45 -0.0 4,244,028 + Pf1N1B4_3829 0.45 -1.2 4,244,028 + Pf1N1B4_3829 0.45 -1.6 4,244,028 + Pf1N1B4_3829 0.45 +0.5 4,244,028 + Pf1N1B4_3829 0.45 -3.2 4,244,028 + Pf1N1B4_3829 0.45 -2.1 4,244,028 + Pf1N1B4_3829 0.45 -1.4 4,244,028 + Pf1N1B4_3829 0.45 -0.7 4,244,028 + Pf1N1B4_3829 0.45 -0.7 4,244,028 + Pf1N1B4_3829 0.45 +0.4 4,244,028 + Pf1N1B4_3829 0.45 -0.8 4,244,029 - Pf1N1B4_3829 0.45 -0.3 4,244,029 - Pf1N1B4_3829 0.45 +1.2 4,244,029 - Pf1N1B4_3829 0.45 -0.7 4,244,029 - Pf1N1B4_3829 0.45 -1.2 4,244,029 - Pf1N1B4_3829 0.45 -0.2 4,244,029 - Pf1N1B4_3829 0.45 +0.1 4,244,029 - Pf1N1B4_3829 0.45 -1.3 4,244,029 - Pf1N1B4_3829 0.45 -0.3 4,244,029 - Pf1N1B4_3829 0.45 -2.7 4,244,029 - Pf1N1B4_3829 0.45 -1.1 4,244,029 - Pf1N1B4_3829 0.45 -2.0 4,244,029 - Pf1N1B4_3829 0.45 -1.0 4,244,029 - Pf1N1B4_3829 0.45 -1.3 4,244,029 - Pf1N1B4_3829 0.45 -1.8 4,244,029 - Pf1N1B4_3829 0.45 -0.0 4,244,030 + Pf1N1B4_3829 0.45 +0.7 4,244,030 + Pf1N1B4_3829 0.45 -0.6 4,244,030 + Pf1N1B4_3829 0.45 +0.2 4,244,031 - Pf1N1B4_3829 0.45 -0.8 4,244,031 - Pf1N1B4_3829 0.45 +0.7 4,244,031 - Pf1N1B4_3829 0.45 -1.3 4,244,031 - Pf1N1B4_3829 0.45 -2.6 4,244,031 - Pf1N1B4_3829 0.45 -1.6 4,244,031 - Pf1N1B4_3829 0.45 -0.3 4,244,040 + Pf1N1B4_3829 0.46 -1.3 4,244,040 + Pf1N1B4_3829 0.46 +1.3 4,244,040 + Pf1N1B4_3829 0.46 +0.7 4,244,041 - +1.7 4,244,041 - Pf1N1B4_3829 0.46 -1.0 4,244,041 - Pf1N1B4_3829 0.46 -1.8 4,244,041 - Pf1N1B4_3829 0.46 -0.5 4,244,049 - Pf1N1B4_3829 0.47 -0.8 4,244,129 + Pf1N1B4_3829 0.55 -0.6 4,244,129 + Pf1N1B4_3829 0.55 -1.7 4,244,129 + Pf1N1B4_3829 0.55 +0.7 4,244,130 - Pf1N1B4_3829 0.55 -2.1 4,244,130 - Pf1N1B4_3829 0.55 -0.8 4,244,130 - Pf1N1B4_3829 0.55 -0.6 4,244,130 - Pf1N1B4_3829 0.55 -0.5 4,244,130 - Pf1N1B4_3829 0.55 -1.3 4,244,130 - Pf1N1B4_3829 0.55 -0.7 4,244,130 - +0.2 4,244,130 - Pf1N1B4_3829 0.55 -1.6 4,244,130 - Pf1N1B4_3829 0.55 +0.2 4,244,130 - Pf1N1B4_3829 0.55 -1.3 4,244,207 + Pf1N1B4_3829 0.63 +0.3 4,244,208 - Pf1N1B4_3829 0.63 -1.3 4,244,230 + -0.3 4,244,230 + Pf1N1B4_3829 0.65 -0.6 4,244,231 - Pf1N1B4_3829 0.66 -1.0 4,244,231 - Pf1N1B4_3829 0.66 -1.3 4,244,231 - Pf1N1B4_3829 0.66 -2.0 4,244,231 - Pf1N1B4_3829 0.66 -1.1 4,244,231 - Pf1N1B4_3829 0.66 -2.3 4,244,237 - Pf1N1B4_3829 0.66 -0.8 4,244,392 - Pf1N1B4_3829 0.82 -1.6 4,244,392 - Pf1N1B4_3829 0.82 -2.1 4,244,392 - Pf1N1B4_3829 0.82 -0.0 4,244,392 - Pf1N1B4_3829 0.82 -1.1 4,244,469 - Pf1N1B4_3829 0.89 -0.6 4,244,549 - -0.8 4,244,549 - -3.7 4,244,620 + -1.6 4,244,620 + -1.7 4,244,620 + +0.0 4,244,621 - -2.2 4,244,621 - +1.0 4,244,621 - -0.3 4,244,621 - -1.3 4,244,621 - -1.6 4,244,621 - -1.1 4,244,621 - -0.3 4,244,636 + +0.7 4,244,636 + -0.8 4,244,636 + -0.6 4,244,636 + -1.1 4,244,636 + +1.5 4,244,637 - -0.4 4,244,637 - -2.1 4,244,825 + Pf1N1B4_3830 0.76 -1.3 4,244,825 + Pf1N1B4_3830 0.76 -0.8 4,244,849 + Pf1N1B4_3830 0.85 +0.1 4,244,850 - Pf1N1B4_3830 0.86 +1.4 4,244,850 - Pf1N1B4_3830 0.86 +0.5 4,244,850 - Pf1N1B4_3830 0.86 +0.7 4,244,855 + Pf1N1B4_3830 0.88 +0.4 4,244,855 + Pf1N1B4_3830 0.88 -0.3 4,244,873 + -1.4 4,244,873 + +0.7 4,244,874 - -2.7 4,245,124 + Pf1N1B4_3831 0.29 +1.7 4,245,143 - Pf1N1B4_3831 0.31 -1.3 4,245,171 + Pf1N1B4_3831 0.34 -0.6 4,245,171 + Pf1N1B4_3831 0.34 -0.7 4,245,171 + Pf1N1B4_3831 0.34 -0.7 4,245,172 - Pf1N1B4_3831 0.34 -0.8 4,245,242 + Pf1N1B4_3831 0.43 -1.6 4,245,242 + Pf1N1B4_3831 0.43 -1.8 4,245,242 + Pf1N1B4_3831 0.43 -1.4 4,245,242 + Pf1N1B4_3831 0.43 -0.5 4,245,242 + Pf1N1B4_3831 0.43 -1.3 4,245,243 - Pf1N1B4_3831 0.43 -3.4 4,245,256 - Pf1N1B4_3831 0.45 -0.5 4,245,400 + Pf1N1B4_3831 0.62 -0.8 4,245,400 + Pf1N1B4_3831 0.62 -0.5 4,245,400 + Pf1N1B4_3831 0.62 +0.2 4,245,400 + Pf1N1B4_3831 0.62 +0.1 4,245,401 - Pf1N1B4_3831 0.62 -2.6 4,245,548 + Pf1N1B4_3831 0.80 +0.7 4,245,548 + Pf1N1B4_3831 0.80 +0.7 4,245,548 + Pf1N1B4_3831 0.80 -0.5 4,245,548 + Pf1N1B4_3831 0.80 -0.3 4,245,548 + Pf1N1B4_3831 0.80 -1.3 4,245,548 + Pf1N1B4_3831 0.80 +0.2 4,245,548 + Pf1N1B4_3831 0.80 -0.4 4,245,548 + Pf1N1B4_3831 0.80 +0.2 4,245,548 + +1.7 4,245,549 - Pf1N1B4_3831 0.80 -1.3 4,245,549 - Pf1N1B4_3831 0.80 -1.4 4,245,563 + Pf1N1B4_3831 0.82 -1.6 4,245,563 + -0.3 4,245,563 + Pf1N1B4_3831 0.82 -0.8 4,245,563 + Pf1N1B4_3831 0.82 -1.4 4,245,564 - Pf1N1B4_3831 0.82 -0.8 4,245,564 - +1.3 4,245,728 + -0.3 4,245,733 + +0.7 4,245,733 + -2.7 4,245,733 + +0.5 4,245,733 + -0.3 4,245,733 + -0.9 4,245,733 + -1.3 4,245,733 + +0.3 4,245,733 + +1.5 4,245,733 + +0.4 4,245,733 + +0.1 4,245,733 + -1.3 4,245,733 + -0.7 4,245,733 + -1.8 4,245,804 + +0.3 4,246,387 + -1.7 4,246,387 + -1.4 4,246,387 + -2.7 4,246,387 + -2.4 4,246,387 + -0.7 4,246,387 + -3.2 4,246,387 + -1.8 4,246,387 + +0.0 4,246,387 + -2.6 4,246,401 + -2.3 4,246,401 + -1.1 4,246,401 + +0.7 4,246,401 + -2.1 4,246,402 - -2.4 4,246,408 + -0.5 4,246,408 + -2.0 4,246,408 + -2.5 4,246,408 + -2.1 4,246,408 + -1.5 4,246,408 + -0.7 4,246,408 + -2.1 4,246,408 + -0.7 4,246,408 + -1.8 4,246,409 - -2.1 4,246,414 + -1.6 4,246,414 + -2.9 4,246,674 + Pf1N1B4_3833 0.17 -1.6
Or see this region's nucleotide sequence