Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3814 and Pf1N1B4_3815 are separated by 228 nucleotides Pf1N1B4_3815 and Pf1N1B4_3816 overlap by 1 nucleotides
Pf1N1B4_3814 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85), at 4,224,615 to 4,225,952
_3814
Pf1N1B4_3815 - FIG002781: Alpha-L-glutamate ligase family protein, at 4,226,181 to 4,227,167
_3815
Pf1N1B4_3816 - FIG139976: hypothetical protein, at 4,227,167 to 4,228,702
_3816
Position (kb)
4226
4227
4228 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4225.375 kb on + strand, within Pf1N1B4_3814 at 4225.376 kb on - strand, within Pf1N1B4_3814 at 4225.458 kb on + strand, within Pf1N1B4_3814 at 4225.458 kb on + strand, within Pf1N1B4_3814 at 4225.582 kb on + strand, within Pf1N1B4_3814 at 4225.582 kb on + strand, within Pf1N1B4_3814 at 4225.582 kb on + strand, within Pf1N1B4_3814 at 4225.663 kb on + strand, within Pf1N1B4_3814 at 4225.663 kb on + strand, within Pf1N1B4_3814 at 4225.663 kb on + strand, within Pf1N1B4_3814 at 4225.664 kb on - strand at 4225.664 kb on - strand, within Pf1N1B4_3814 at 4225.664 kb on - strand, within Pf1N1B4_3814 at 4225.664 kb on - strand, within Pf1N1B4_3814 at 4225.664 kb on - strand, within Pf1N1B4_3814 at 4225.664 kb on - strand at 4225.664 kb on - strand at 4225.826 kb on - strand at 4225.901 kb on - strand at 4225.992 kb on + strand at 4225.992 kb on + strand at 4225.992 kb on + strand at 4225.992 kb on + strand at 4226.015 kb on + strand at 4226.015 kb on + strand at 4226.016 kb on - strand at 4226.036 kb on + strand at 4226.036 kb on + strand at 4226.037 kb on - strand at 4226.174 kb on + strand at 4226.202 kb on - strand at 4226.413 kb on + strand, within Pf1N1B4_3815 at 4226.414 kb on - strand, within Pf1N1B4_3815 at 4226.414 kb on - strand, within Pf1N1B4_3815 at 4226.414 kb on - strand, within Pf1N1B4_3815 at 4226.414 kb on - strand, within Pf1N1B4_3815 at 4226.434 kb on + strand, within Pf1N1B4_3815 at 4226.435 kb on - strand, within Pf1N1B4_3815 at 4226.435 kb on - strand, within Pf1N1B4_3815 at 4226.435 kb on - strand, within Pf1N1B4_3815 at 4226.435 kb on - strand, within Pf1N1B4_3815 at 4226.435 kb on - strand, within Pf1N1B4_3815 at 4226.435 kb on - strand, within Pf1N1B4_3815 at 4226.698 kb on + strand, within Pf1N1B4_3815 at 4226.699 kb on - strand, within Pf1N1B4_3815 at 4226.699 kb on - strand, within Pf1N1B4_3815 at 4226.699 kb on - strand, within Pf1N1B4_3815 at 4226.699 kb on - strand, within Pf1N1B4_3815 at 4226.699 kb on - strand, within Pf1N1B4_3815 at 4226.699 kb on - strand, within Pf1N1B4_3815 at 4226.776 kb on + strand, within Pf1N1B4_3815 at 4226.776 kb on + strand at 4227.079 kb on + strand at 4227.079 kb on + strand at 4227.080 kb on - strand at 4227.080 kb on - strand at 4227.080 kb on - strand at 4227.080 kb on - strand at 4227.091 kb on + strand at 4227.091 kb on + strand at 4227.091 kb on + strand at 4227.091 kb on + strand at 4227.091 kb on + strand at 4227.092 kb on - strand at 4227.092 kb on - strand at 4227.092 kb on - strand at 4227.092 kb on - strand at 4227.092 kb on - strand at 4227.297 kb on + strand at 4227.297 kb on + strand at 4227.297 kb on + strand at 4227.297 kb on + strand at 4227.297 kb on + strand at 4227.298 kb on - strand at 4227.298 kb on - strand at 4227.576 kb on + strand, within Pf1N1B4_3816 at 4227.576 kb on + strand at 4227.576 kb on + strand, within Pf1N1B4_3816 at 4227.576 kb on + strand, within Pf1N1B4_3816 at 4227.576 kb on + strand, within Pf1N1B4_3816 at 4227.577 kb on - strand, within Pf1N1B4_3816 at 4227.577 kb on - strand, within Pf1N1B4_3816 at 4227.577 kb on - strand at 4227.577 kb on - strand at 4227.577 kb on - strand at 4227.613 kb on + strand, within Pf1N1B4_3816 at 4227.613 kb on + strand at 4227.613 kb on + strand, within Pf1N1B4_3816 at 4227.614 kb on - strand, within Pf1N1B4_3816 at 4227.614 kb on - strand, within Pf1N1B4_3816 at 4227.614 kb on - strand, within Pf1N1B4_3816 at 4227.614 kb on - strand, within Pf1N1B4_3816 at 4227.614 kb on - strand, within Pf1N1B4_3816 at 4227.617 kb on + strand, within Pf1N1B4_3816 at 4227.617 kb on + strand, within Pf1N1B4_3816 at 4227.617 kb on + strand, within Pf1N1B4_3816 at 4227.617 kb on + strand, within Pf1N1B4_3816 at 4227.617 kb on + strand, within Pf1N1B4_3816 at 4227.792 kb on + strand, within Pf1N1B4_3816 at 4227.793 kb on - strand, within Pf1N1B4_3816 at 4227.801 kb on + strand, within Pf1N1B4_3816 at 4227.993 kb on + strand, within Pf1N1B4_3816 at 4227.994 kb on - strand, within Pf1N1B4_3816 at 4228.106 kb on + strand at 4228.107 kb on - strand, within Pf1N1B4_3816 at 4228.107 kb on - strand, within Pf1N1B4_3816
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,225,375 + Pf1N1B4_3814 0.57 +0.5 4,225,376 - Pf1N1B4_3814 0.57 +1.2 4,225,458 + Pf1N1B4_3814 0.63 +1.6 4,225,458 + Pf1N1B4_3814 0.63 -0.6 4,225,582 + Pf1N1B4_3814 0.72 +0.8 4,225,582 + Pf1N1B4_3814 0.72 -0.0 4,225,582 + Pf1N1B4_3814 0.72 -1.6 4,225,663 + Pf1N1B4_3814 0.78 +0.0 4,225,663 + Pf1N1B4_3814 0.78 -1.4 4,225,663 + Pf1N1B4_3814 0.78 -1.6 4,225,664 - -0.2 4,225,664 - Pf1N1B4_3814 0.78 -0.3 4,225,664 - Pf1N1B4_3814 0.78 -0.1 4,225,664 - Pf1N1B4_3814 0.78 +0.3 4,225,664 - Pf1N1B4_3814 0.78 -1.8 4,225,664 - -0.2 4,225,664 - -0.2 4,225,826 - -0.4 4,225,901 - +1.8 4,225,992 + +0.5 4,225,992 + +0.3 4,225,992 + -0.6 4,225,992 + -0.1 4,226,015 + +0.0 4,226,015 + -0.5 4,226,016 - +1.2 4,226,036 + -0.6 4,226,036 + +0.0 4,226,037 - -1.8 4,226,174 + +1.8 4,226,202 - +0.8 4,226,413 + Pf1N1B4_3815 0.24 -0.2 4,226,414 - Pf1N1B4_3815 0.24 -2.2 4,226,414 - Pf1N1B4_3815 0.24 -0.2 4,226,414 - Pf1N1B4_3815 0.24 -1.1 4,226,414 - Pf1N1B4_3815 0.24 -1.2 4,226,434 + Pf1N1B4_3815 0.26 +0.8 4,226,435 - Pf1N1B4_3815 0.26 -1.6 4,226,435 - Pf1N1B4_3815 0.26 -1.2 4,226,435 - Pf1N1B4_3815 0.26 +1.3 4,226,435 - Pf1N1B4_3815 0.26 -0.8 4,226,435 - Pf1N1B4_3815 0.26 +0.4 4,226,435 - Pf1N1B4_3815 0.26 +0.8 4,226,698 + Pf1N1B4_3815 0.52 -1.8 4,226,699 - Pf1N1B4_3815 0.52 -0.8 4,226,699 - Pf1N1B4_3815 0.52 -0.8 4,226,699 - Pf1N1B4_3815 0.52 -1.2 4,226,699 - Pf1N1B4_3815 0.52 -0.6 4,226,699 - Pf1N1B4_3815 0.52 +0.8 4,226,699 - Pf1N1B4_3815 0.52 -0.8 4,226,776 + Pf1N1B4_3815 0.60 -0.2 4,226,776 + +0.8 4,227,079 + +1.2 4,227,079 + -2.1 4,227,080 - -1.9 4,227,080 - -2.2 4,227,080 - -0.6 4,227,080 - -0.8 4,227,091 + -0.2 4,227,091 + -2.2 4,227,091 + -1.8 4,227,091 + -1.2 4,227,091 + -0.2 4,227,092 - -1.5 4,227,092 - -2.4 4,227,092 - -1.6 4,227,092 - -1.8 4,227,092 - -0.8 4,227,297 + -0.8 4,227,297 + -1.6 4,227,297 + -1.6 4,227,297 + -2.2 4,227,297 + -2.2 4,227,298 - -1.8 4,227,298 - -2.4 4,227,576 + Pf1N1B4_3816 0.27 +0.7 4,227,576 + +0.7 4,227,576 + Pf1N1B4_3816 0.27 +0.7 4,227,576 + Pf1N1B4_3816 0.27 -1.1 4,227,576 + Pf1N1B4_3816 0.27 -0.5 4,227,577 - Pf1N1B4_3816 0.27 -1.8 4,227,577 - Pf1N1B4_3816 0.27 -0.8 4,227,577 - -0.3 4,227,577 - -1.3 4,227,577 - -1.3 4,227,613 + Pf1N1B4_3816 0.29 -0.7 4,227,613 + -1.6 4,227,613 + Pf1N1B4_3816 0.29 -0.8 4,227,614 - Pf1N1B4_3816 0.29 -0.1 4,227,614 - Pf1N1B4_3816 0.29 -1.3 4,227,614 - Pf1N1B4_3816 0.29 -0.3 4,227,614 - Pf1N1B4_3816 0.29 -0.8 4,227,614 - Pf1N1B4_3816 0.29 -1.8 4,227,617 + Pf1N1B4_3816 0.29 -0.7 4,227,617 + Pf1N1B4_3816 0.29 -1.1 4,227,617 + Pf1N1B4_3816 0.29 -1.8 4,227,617 + Pf1N1B4_3816 0.29 -2.3 4,227,617 + Pf1N1B4_3816 0.29 -0.7 4,227,792 + Pf1N1B4_3816 0.41 -1.4 4,227,793 - Pf1N1B4_3816 0.41 -1.6 4,227,801 + Pf1N1B4_3816 0.41 +0.2 4,227,993 + Pf1N1B4_3816 0.54 -2.3 4,227,994 - Pf1N1B4_3816 0.54 -2.3 4,228,106 + -0.8 4,228,107 - Pf1N1B4_3816 0.61 -0.3 4,228,107 - Pf1N1B4_3816 0.61 -2.5
Or see this region's nucleotide sequence