Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3762 and Pf1N1B4_3763 are separated by 65 nucleotides Pf1N1B4_3763 and Pf1N1B4_3764 are separated by 120 nucleotides Pf1N1B4_3764 and Pf1N1B4_3765 are separated by 88 nucleotides Pf1N1B4_3765 and Pf1N1B4_3766 overlap by 4 nucleotides
Pf1N1B4_3762 - FIG137887: membrane protein related to purine degradation, at 4,173,333 to 4,174,562
_3762
Pf1N1B4_3763 - Ureidoglycolate hydrolase (EC 3.5.3.19), at 4,174,628 to 4,175,131
_3763
Pf1N1B4_3764 - Allantoicase (EC 3.5.3.4), at 4,175,252 to 4,176,247
_3764
Pf1N1B4_3765 - 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) decarboxylase, at 4,176,336 to 4,176,851
_3765
Pf1N1B4_3766 - Uricase (urate oxidase) (EC 1.7.3.3), at 4,176,848 to 4,177,774
_3766
Position (kb)
4175
4176
4177 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4174.282 kb on + strand, within Pf1N1B4_3762 at 4174.282 kb on + strand, within Pf1N1B4_3762 at 4174.327 kb on + strand, within Pf1N1B4_3762 at 4174.328 kb on - strand, within Pf1N1B4_3762 at 4174.328 kb on - strand, within Pf1N1B4_3762 at 4174.328 kb on - strand, within Pf1N1B4_3762 at 4174.399 kb on + strand at 4174.400 kb on - strand, within Pf1N1B4_3762 at 4174.490 kb on - strand at 4174.630 kb on - strand at 4174.630 kb on - strand at 4174.630 kb on - strand at 4174.687 kb on + strand, within Pf1N1B4_3763 at 4174.688 kb on - strand, within Pf1N1B4_3763 at 4174.773 kb on + strand, within Pf1N1B4_3763 at 4174.773 kb on + strand, within Pf1N1B4_3763 at 4174.773 kb on + strand, within Pf1N1B4_3763 at 4174.773 kb on + strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.774 kb on - strand, within Pf1N1B4_3763 at 4174.777 kb on + strand, within Pf1N1B4_3763 at 4174.777 kb on + strand, within Pf1N1B4_3763 at 4174.777 kb on + strand, within Pf1N1B4_3763 at 4174.777 kb on + strand, within Pf1N1B4_3763 at 4174.778 kb on - strand, within Pf1N1B4_3763 at 4174.778 kb on - strand, within Pf1N1B4_3763 at 4174.778 kb on - strand, within Pf1N1B4_3763 at 4174.778 kb on - strand, within Pf1N1B4_3763 at 4175.188 kb on + strand at 4175.253 kb on + strand at 4175.253 kb on + strand at 4175.253 kb on + strand at 4175.253 kb on + strand at 4175.254 kb on - strand at 4175.254 kb on - strand at 4175.257 kb on + strand at 4175.258 kb on - strand at 4175.362 kb on + strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.363 kb on - strand, within Pf1N1B4_3764 at 4175.488 kb on - strand, within Pf1N1B4_3764 at 4175.579 kb on - strand at 4175.845 kb on - strand, within Pf1N1B4_3764 at 4176.237 kb on + strand at 4176.237 kb on + strand at 4176.264 kb on + strand at 4176.265 kb on - strand at 4176.268 kb on + strand at 4176.268 kb on + strand at 4176.268 kb on + strand at 4176.268 kb on + strand at 4176.269 kb on - strand at 4176.339 kb on + strand at 4176.339 kb on + strand at 4176.340 kb on - strand at 4176.466 kb on - strand, within Pf1N1B4_3765 at 4176.502 kb on + strand, within Pf1N1B4_3765 at 4176.503 kb on - strand, within Pf1N1B4_3765 at 4176.503 kb on - strand, within Pf1N1B4_3765 at 4176.503 kb on - strand at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.778 kb on + strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.779 kb on - strand, within Pf1N1B4_3765 at 4176.937 kb on + strand at 4177.044 kb on + strand, within Pf1N1B4_3766 at 4177.044 kb on + strand, within Pf1N1B4_3766 at 4177.045 kb on - strand, within Pf1N1B4_3766 at 4177.144 kb on - strand at 4177.209 kb on + strand, within Pf1N1B4_3766 at 4177.209 kb on + strand, within Pf1N1B4_3766 at 4177.209 kb on + strand, within Pf1N1B4_3766 at 4177.210 kb on - strand, within Pf1N1B4_3766 at 4177.210 kb on - strand, within Pf1N1B4_3766
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,174,282 + Pf1N1B4_3762 0.77 +0.2 4,174,282 + Pf1N1B4_3762 0.77 +0.7 4,174,327 + Pf1N1B4_3762 0.81 -0.4 4,174,328 - Pf1N1B4_3762 0.81 -1.1 4,174,328 - Pf1N1B4_3762 0.81 +0.1 4,174,328 - Pf1N1B4_3762 0.81 +0.5 4,174,399 + -0.2 4,174,400 - Pf1N1B4_3762 0.87 -0.2 4,174,490 - -0.4 4,174,630 - +0.4 4,174,630 - -1.2 4,174,630 - +1.3 4,174,687 + Pf1N1B4_3763 0.12 +0.3 4,174,688 - Pf1N1B4_3763 0.12 +1.2 4,174,773 + Pf1N1B4_3763 0.29 +0.5 4,174,773 + Pf1N1B4_3763 0.29 +0.2 4,174,773 + Pf1N1B4_3763 0.29 -2.8 4,174,773 + Pf1N1B4_3763 0.29 +0.4 4,174,774 - Pf1N1B4_3763 0.29 -1.2 4,174,774 - Pf1N1B4_3763 0.29 -0.8 4,174,774 - Pf1N1B4_3763 0.29 +0.0 4,174,774 - Pf1N1B4_3763 0.29 -1.0 4,174,774 - Pf1N1B4_3763 0.29 +1.5 4,174,774 - Pf1N1B4_3763 0.29 -2.0 4,174,777 + Pf1N1B4_3763 0.30 -1.2 4,174,777 + Pf1N1B4_3763 0.30 -1.5 4,174,777 + Pf1N1B4_3763 0.30 +1.0 4,174,777 + Pf1N1B4_3763 0.30 +0.8 4,174,778 - Pf1N1B4_3763 0.30 +0.5 4,174,778 - Pf1N1B4_3763 0.30 -0.8 4,174,778 - Pf1N1B4_3763 0.30 -0.1 4,174,778 - Pf1N1B4_3763 0.30 +0.1 4,175,188 + +0.8 4,175,253 + +0.8 4,175,253 + +0.8 4,175,253 + +0.8 4,175,253 + -0.8 4,175,254 - +0.2 4,175,254 - +0.1 4,175,257 + +0.2 4,175,258 - +1.1 4,175,362 + Pf1N1B4_3764 0.11 -0.4 4,175,363 - Pf1N1B4_3764 0.11 -2.2 4,175,363 - Pf1N1B4_3764 0.11 +0.0 4,175,363 - Pf1N1B4_3764 0.11 -1.0 4,175,363 - Pf1N1B4_3764 0.11 -1.5 4,175,363 - Pf1N1B4_3764 0.11 -3.0 4,175,488 - Pf1N1B4_3764 0.24 -0.2 4,175,579 - -0.2 4,175,845 - Pf1N1B4_3764 0.60 -0.0 4,176,237 + +1.3 4,176,237 + -1.5 4,176,264 + -0.8 4,176,265 - +1.8 4,176,268 + -0.6 4,176,268 + +0.5 4,176,268 + +1.3 4,176,268 + -0.4 4,176,269 - -1.2 4,176,339 + -0.4 4,176,339 + -0.5 4,176,340 - +0.6 4,176,466 - Pf1N1B4_3765 0.25 -2.5 4,176,502 + Pf1N1B4_3765 0.32 +0.8 4,176,503 - Pf1N1B4_3765 0.32 +0.8 4,176,503 - Pf1N1B4_3765 0.32 +0.0 4,176,503 - +0.8 4,176,778 + Pf1N1B4_3765 0.86 -0.5 4,176,778 + Pf1N1B4_3765 0.86 +0.8 4,176,778 + Pf1N1B4_3765 0.86 +0.1 4,176,778 + Pf1N1B4_3765 0.86 +1.1 4,176,778 + Pf1N1B4_3765 0.86 +0.2 4,176,778 + Pf1N1B4_3765 0.86 -0.0 4,176,778 + Pf1N1B4_3765 0.86 -0.4 4,176,778 + Pf1N1B4_3765 0.86 +0.8 4,176,778 + Pf1N1B4_3765 0.86 +0.2 4,176,779 - Pf1N1B4_3765 0.86 -1.0 4,176,779 - Pf1N1B4_3765 0.86 -0.4 4,176,779 - Pf1N1B4_3765 0.86 +0.1 4,176,779 - Pf1N1B4_3765 0.86 +1.1 4,176,779 - Pf1N1B4_3765 0.86 -1.2 4,176,937 + +0.2 4,177,044 + Pf1N1B4_3766 0.21 -0.2 4,177,044 + Pf1N1B4_3766 0.21 -0.4 4,177,045 - Pf1N1B4_3766 0.21 +0.5 4,177,144 - +0.5 4,177,209 + Pf1N1B4_3766 0.39 -0.4 4,177,209 + Pf1N1B4_3766 0.39 +1.0 4,177,209 + Pf1N1B4_3766 0.39 +0.2 4,177,210 - Pf1N1B4_3766 0.39 +0.4 4,177,210 - Pf1N1B4_3766 0.39 +2.8
Or see this region's nucleotide sequence