Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3750 and Pf1N1B4_3751 are separated by 120 nucleotides Pf1N1B4_3751 and Pf1N1B4_3752 are separated by 206 nucleotides Pf1N1B4_3752 and Pf1N1B4_3753 are separated by 209 nucleotides
Pf1N1B4_3750 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 4,161,583 to 4,163,670
_3750
Pf1N1B4_3751 - FIG00953060: hypothetical protein, at 4,163,791 to 4,163,967
_3751
Pf1N1B4_3752 - Aspartyl aminopeptidase, at 4,164,174 to 4,165,463
_3752
Pf1N1B4_3753 - Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70), at 4,165,673 to 4,166,308
_3753
Position (kb) 4164
4165
4166 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4163.515 kb on + strand at 4163.516 kb on - strand at 4163.751 kb on + strand at 4163.752 kb on - strand at 4163.752 kb on - strand at 4163.792 kb on + strand at 4163.792 kb on + strand at 4163.792 kb on + strand at 4163.819 kb on + strand at 4163.819 kb on + strand, within Pf1N1B4_3751 at 4163.819 kb on + strand, within Pf1N1B4_3751 at 4163.820 kb on - strand, within Pf1N1B4_3751 at 4163.820 kb on - strand at 4163.991 kb on + strand at 4164.002 kb on + strand at 4164.002 kb on + strand at 4164.104 kb on + strand at 4164.104 kb on + strand at 4164.110 kb on + strand at 4164.111 kb on - strand at 4164.140 kb on + strand at 4164.175 kb on + strand at 4164.196 kb on + strand at 4164.196 kb on + strand at 4164.196 kb on + strand at 4164.196 kb on + strand at 4164.197 kb on - strand at 4164.197 kb on - strand at 4164.197 kb on - strand at 4164.197 kb on - strand at 4164.197 kb on - strand at 4164.335 kb on + strand, within Pf1N1B4_3752 at 4164.335 kb on + strand, within Pf1N1B4_3752 at 4164.335 kb on + strand, within Pf1N1B4_3752 at 4164.335 kb on + strand, within Pf1N1B4_3752 at 4164.336 kb on - strand, within Pf1N1B4_3752 at 4164.336 kb on - strand, within Pf1N1B4_3752 at 4164.336 kb on - strand, within Pf1N1B4_3752 at 4164.336 kb on - strand, within Pf1N1B4_3752 at 4164.381 kb on + strand, within Pf1N1B4_3752 at 4164.381 kb on + strand, within Pf1N1B4_3752 at 4164.381 kb on + strand, within Pf1N1B4_3752 at 4164.382 kb on - strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.518 kb on - strand at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.579 kb on + strand, within Pf1N1B4_3752 at 4164.740 kb on - strand, within Pf1N1B4_3752 at 4164.755 kb on + strand, within Pf1N1B4_3752 at 4164.756 kb on - strand, within Pf1N1B4_3752 at 4164.820 kb on + strand, within Pf1N1B4_3752 at 4164.820 kb on + strand, within Pf1N1B4_3752 at 4164.820 kb on + strand, within Pf1N1B4_3752 at 4164.820 kb on + strand, within Pf1N1B4_3752 at 4164.820 kb on + strand, within Pf1N1B4_3752 at 4164.821 kb on - strand, within Pf1N1B4_3752 at 4164.821 kb on - strand, within Pf1N1B4_3752 at 4164.821 kb on - strand at 4164.821 kb on - strand, within Pf1N1B4_3752 at 4164.821 kb on - strand, within Pf1N1B4_3752 at 4164.821 kb on - strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.846 kb on + strand, within Pf1N1B4_3752 at 4164.847 kb on - strand, within Pf1N1B4_3752 at 4164.847 kb on - strand, within Pf1N1B4_3752 at 4164.847 kb on - strand, within Pf1N1B4_3752 at 4164.847 kb on - strand, within Pf1N1B4_3752 at 4164.847 kb on - strand, within Pf1N1B4_3752 at 4164.978 kb on - strand, within Pf1N1B4_3752 at 4165.025 kb on + strand, within Pf1N1B4_3752 at 4165.025 kb on + strand, within Pf1N1B4_3752 at 4165.025 kb on + strand, within Pf1N1B4_3752 at 4165.138 kb on + strand, within Pf1N1B4_3752 at 4165.138 kb on + strand, within Pf1N1B4_3752 at 4165.138 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.306 kb on + strand, within Pf1N1B4_3752 at 4165.307 kb on - strand, within Pf1N1B4_3752 at 4165.307 kb on - strand, within Pf1N1B4_3752 at 4165.307 kb on - strand, within Pf1N1B4_3752 at 4165.307 kb on - strand, within Pf1N1B4_3752 at 4165.307 kb on - strand, within Pf1N1B4_3752 at 4165.307 kb on - strand, within Pf1N1B4_3752 at 4165.307 kb on - strand, within Pf1N1B4_3752 at 4165.309 kb on + strand, within Pf1N1B4_3752 at 4165.309 kb on + strand, within Pf1N1B4_3752 at 4165.358 kb on + strand at 4165.358 kb on + strand at 4165.359 kb on - strand at 4165.359 kb on - strand at 4165.463 kb on + strand at 4165.463 kb on + strand at 4165.494 kb on + strand at 4165.499 kb on + strand at 4165.499 kb on + strand at 4165.499 kb on + strand at 4165.499 kb on + strand at 4165.499 kb on + strand at 4165.499 kb on + strand at 4165.499 kb on + strand at 4165.500 kb on - strand at 4165.500 kb on - strand at 4165.545 kb on + strand at 4165.545 kb on + strand at 4165.546 kb on - strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.609 kb on + strand at 4165.610 kb on - strand at 4165.610 kb on - strand at 4165.610 kb on - strand at 4165.610 kb on - strand at 4165.610 kb on - strand at 4165.610 kb on - strand at 4165.791 kb on + strand, within Pf1N1B4_3753 at 4165.791 kb on + strand, within Pf1N1B4_3753 at 4165.791 kb on + strand, within Pf1N1B4_3753 at 4165.806 kb on - strand, within Pf1N1B4_3753 at 4165.821 kb on - strand, within Pf1N1B4_3753 at 4165.821 kb on - strand, within Pf1N1B4_3753 at 4165.831 kb on + strand, within Pf1N1B4_3753 at 4165.831 kb on + strand, within Pf1N1B4_3753 at 4165.831 kb on + strand, within Pf1N1B4_3753 at 4165.831 kb on + strand, within Pf1N1B4_3753 at 4165.831 kb on + strand, within Pf1N1B4_3753 at 4165.832 kb on - strand, within Pf1N1B4_3753 at 4165.832 kb on - strand, within Pf1N1B4_3753 at 4165.832 kb on - strand, within Pf1N1B4_3753 at 4165.832 kb on - strand, within Pf1N1B4_3753 at 4166.043 kb on + strand, within Pf1N1B4_3753 at 4166.309 kb on + strand at 4166.323 kb on - strand at 4166.443 kb on - strand at 4166.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,163,515 + +3.1 4,163,516 - +0.1 4,163,751 + -1.0 4,163,752 - -0.2 4,163,752 - +0.8 4,163,792 + +0.8 4,163,792 + -1.2 4,163,792 + +1.8 4,163,819 + +1.8 4,163,819 + Pf1N1B4_3751 0.16 +1.2 4,163,819 + Pf1N1B4_3751 0.16 +1.3 4,163,820 - Pf1N1B4_3751 0.16 -1.4 4,163,820 - -0.8 4,163,991 + -0.5 4,164,002 + -0.9 4,164,002 + +0.3 4,164,104 + -0.1 4,164,104 + +2.3 4,164,110 + -2.0 4,164,111 - -0.3 4,164,140 + -1.1 4,164,175 + -0.5 4,164,196 + -1.5 4,164,196 + -0.1 4,164,196 + -0.8 4,164,196 + +0.4 4,164,197 - -0.2 4,164,197 - +0.4 4,164,197 - +0.8 4,164,197 - -2.2 4,164,197 - +1.0 4,164,335 + Pf1N1B4_3752 0.12 +0.1 4,164,335 + Pf1N1B4_3752 0.12 +1.2 4,164,335 + Pf1N1B4_3752 0.12 +0.1 4,164,335 + Pf1N1B4_3752 0.12 -0.5 4,164,336 - Pf1N1B4_3752 0.13 -0.1 4,164,336 - Pf1N1B4_3752 0.13 +0.4 4,164,336 - Pf1N1B4_3752 0.13 +1.3 4,164,336 - Pf1N1B4_3752 0.13 +0.8 4,164,381 + Pf1N1B4_3752 0.16 +0.2 4,164,381 + Pf1N1B4_3752 0.16 -1.9 4,164,381 + Pf1N1B4_3752 0.16 +0.4 4,164,382 - Pf1N1B4_3752 0.16 -0.3 4,164,517 + Pf1N1B4_3752 0.27 -0.2 4,164,517 + Pf1N1B4_3752 0.27 +0.6 4,164,517 + Pf1N1B4_3752 0.27 +1.4 4,164,517 + Pf1N1B4_3752 0.27 +1.9 4,164,517 + Pf1N1B4_3752 0.27 -0.4 4,164,517 + Pf1N1B4_3752 0.27 -0.2 4,164,518 - -0.2 4,164,518 - Pf1N1B4_3752 0.27 -0.4 4,164,518 - Pf1N1B4_3752 0.27 -0.3 4,164,518 - Pf1N1B4_3752 0.27 +1.5 4,164,518 - Pf1N1B4_3752 0.27 +0.4 4,164,518 - Pf1N1B4_3752 0.27 -0.1 4,164,518 - Pf1N1B4_3752 0.27 +0.9 4,164,518 - Pf1N1B4_3752 0.27 +0.3 4,164,579 + Pf1N1B4_3752 0.31 +2.4 4,164,740 - Pf1N1B4_3752 0.44 +0.9 4,164,755 + Pf1N1B4_3752 0.45 +0.9 4,164,756 - Pf1N1B4_3752 0.45 +1.3 4,164,820 + Pf1N1B4_3752 0.50 +1.7 4,164,820 + Pf1N1B4_3752 0.50 +1.0 4,164,820 + Pf1N1B4_3752 0.50 +0.3 4,164,820 + Pf1N1B4_3752 0.50 -0.5 4,164,820 + Pf1N1B4_3752 0.50 +0.5 4,164,821 - Pf1N1B4_3752 0.50 +0.5 4,164,821 - Pf1N1B4_3752 0.50 +0.1 4,164,821 - +0.2 4,164,821 - Pf1N1B4_3752 0.50 +0.5 4,164,821 - Pf1N1B4_3752 0.50 -0.1 4,164,821 - Pf1N1B4_3752 0.50 -0.2 4,164,846 + Pf1N1B4_3752 0.52 +0.5 4,164,846 + Pf1N1B4_3752 0.52 +0.4 4,164,846 + Pf1N1B4_3752 0.52 -1.5 4,164,846 + Pf1N1B4_3752 0.52 -0.6 4,164,846 + Pf1N1B4_3752 0.52 +0.6 4,164,846 + Pf1N1B4_3752 0.52 -0.8 4,164,846 + Pf1N1B4_3752 0.52 -0.2 4,164,846 + Pf1N1B4_3752 0.52 +0.4 4,164,846 + Pf1N1B4_3752 0.52 -2.0 4,164,846 + Pf1N1B4_3752 0.52 +0.8 4,164,847 - Pf1N1B4_3752 0.52 +0.3 4,164,847 - Pf1N1B4_3752 0.52 -0.5 4,164,847 - Pf1N1B4_3752 0.52 -0.5 4,164,847 - Pf1N1B4_3752 0.52 +1.5 4,164,847 - Pf1N1B4_3752 0.52 -0.8 4,164,978 - Pf1N1B4_3752 0.62 -0.8 4,165,025 + Pf1N1B4_3752 0.66 -0.6 4,165,025 + Pf1N1B4_3752 0.66 -0.3 4,165,025 + Pf1N1B4_3752 0.66 -0.2 4,165,138 + Pf1N1B4_3752 0.75 +0.2 4,165,138 + Pf1N1B4_3752 0.75 +2.0 4,165,138 + Pf1N1B4_3752 0.75 -1.9 4,165,306 + Pf1N1B4_3752 0.88 +0.2 4,165,306 + Pf1N1B4_3752 0.88 +0.4 4,165,306 + Pf1N1B4_3752 0.88 +0.2 4,165,306 + Pf1N1B4_3752 0.88 -0.6 4,165,306 + Pf1N1B4_3752 0.88 -1.5 4,165,306 + Pf1N1B4_3752 0.88 -0.3 4,165,306 + Pf1N1B4_3752 0.88 -0.5 4,165,306 + Pf1N1B4_3752 0.88 +0.5 4,165,306 + Pf1N1B4_3752 0.88 -0.2 4,165,306 + Pf1N1B4_3752 0.88 +0.5 4,165,306 + Pf1N1B4_3752 0.88 +0.2 4,165,306 + Pf1N1B4_3752 0.88 -1.5 4,165,306 + Pf1N1B4_3752 0.88 +0.8 4,165,306 + Pf1N1B4_3752 0.88 +0.4 4,165,306 + Pf1N1B4_3752 0.88 -1.2 4,165,306 + Pf1N1B4_3752 0.88 +0.8 4,165,306 + Pf1N1B4_3752 0.88 +0.2 4,165,306 + Pf1N1B4_3752 0.88 +0.1 4,165,306 + Pf1N1B4_3752 0.88 +2.4 4,165,306 + Pf1N1B4_3752 0.88 +0.2 4,165,306 + Pf1N1B4_3752 0.88 +1.0 4,165,306 + Pf1N1B4_3752 0.88 +0.6 4,165,307 - Pf1N1B4_3752 0.88 -2.4 4,165,307 - Pf1N1B4_3752 0.88 +2.1 4,165,307 - Pf1N1B4_3752 0.88 -1.4 4,165,307 - Pf1N1B4_3752 0.88 +0.8 4,165,307 - Pf1N1B4_3752 0.88 -0.2 4,165,307 - Pf1N1B4_3752 0.88 -1.8 4,165,307 - Pf1N1B4_3752 0.88 +0.3 4,165,309 + Pf1N1B4_3752 0.88 -1.2 4,165,309 + Pf1N1B4_3752 0.88 -2.8 4,165,358 + -1.0 4,165,358 + +0.1 4,165,359 - -0.2 4,165,359 - +1.8 4,165,463 + -0.8 4,165,463 + +0.4 4,165,494 + +0.1 4,165,499 + -0.2 4,165,499 + +0.7 4,165,499 + -0.1 4,165,499 + -0.4 4,165,499 + -0.5 4,165,499 + -1.2 4,165,499 + +0.5 4,165,500 - -2.1 4,165,500 - +1.5 4,165,545 + -1.0 4,165,545 + -0.6 4,165,546 - -0.8 4,165,609 + +0.8 4,165,609 + -1.0 4,165,609 + +0.1 4,165,609 + +1.5 4,165,609 + +0.8 4,165,609 + -0.2 4,165,609 + -0.2 4,165,609 + +1.8 4,165,609 + -1.3 4,165,609 + +0.2 4,165,609 + +0.5 4,165,609 + +0.8 4,165,609 + -0.2 4,165,609 + -0.2 4,165,609 + -2.0 4,165,609 + -0.3 4,165,609 + -0.4 4,165,609 + -1.4 4,165,609 + -0.0 4,165,609 + -0.4 4,165,609 + +0.8 4,165,609 + -0.8 4,165,609 + +0.1 4,165,609 + -0.2 4,165,609 + +0.2 4,165,609 + -1.5 4,165,610 - +0.1 4,165,610 - +0.4 4,165,610 - +1.7 4,165,610 - +0.8 4,165,610 - +1.5 4,165,610 - +0.5 4,165,791 + Pf1N1B4_3753 0.19 -1.2 4,165,791 + Pf1N1B4_3753 0.19 +1.3 4,165,791 + Pf1N1B4_3753 0.19 +0.8 4,165,806 - Pf1N1B4_3753 0.21 +1.1 4,165,821 - Pf1N1B4_3753 0.23 +0.6 4,165,821 - Pf1N1B4_3753 0.23 -1.5 4,165,831 + Pf1N1B4_3753 0.25 +1.1 4,165,831 + Pf1N1B4_3753 0.25 -1.5 4,165,831 + Pf1N1B4_3753 0.25 +0.8 4,165,831 + Pf1N1B4_3753 0.25 +0.5 4,165,831 + Pf1N1B4_3753 0.25 -1.2 4,165,832 - Pf1N1B4_3753 0.25 -0.5 4,165,832 - Pf1N1B4_3753 0.25 -0.8 4,165,832 - Pf1N1B4_3753 0.25 -0.6 4,165,832 - Pf1N1B4_3753 0.25 +0.0 4,166,043 + Pf1N1B4_3753 0.58 -0.3 4,166,309 + +0.8 4,166,323 - -2.4 4,166,443 - -1.2 4,166,443 - +0.8
Or see this region's nucleotide sequence