Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3465 and Pf1N1B4_3466 are separated by 63 nucleotides Pf1N1B4_3466 and Pf1N1B4_3467 are separated by 88 nucleotides Pf1N1B4_3467 and Pf1N1B4_3468 are separated by 47 nucleotides Pf1N1B4_3468 and Pf1N1B4_3469 are separated by 169 nucleotides
Pf1N1B4_3465 - Transporter, MFS superfamily, at 3,871,796 to 3,873,178
_3465
Pf1N1B4_3466 - FIG00955605: hypothetical protein, at 3,873,242 to 3,873,988
_3466
Pf1N1B4_3467 - Flagellar biosynthesis protein FlgN, at 3,874,077 to 3,874,544
_3467
Pf1N1B4_3468 - Negative regulator of flagellin synthesis FlgM, at 3,874,592 to 3,874,918
_3468
Pf1N1B4_3469 - Flagellar basal-body P-ring formation protein FlgA, at 3,875,088 to 3,875,786
_3469
Position (kb)
3874
3875 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3873.240 kb on + strand at 3873.288 kb on + strand at 3873.288 kb on + strand at 3873.289 kb on - strand at 3873.289 kb on - strand at 3873.289 kb on - strand at 3873.385 kb on - strand, within Pf1N1B4_3466 at 3873.624 kb on + strand at 3873.625 kb on - strand, within Pf1N1B4_3466 at 3873.654 kb on + strand, within Pf1N1B4_3466 at 3873.716 kb on + strand, within Pf1N1B4_3466 at 3873.837 kb on + strand, within Pf1N1B4_3466 at 3873.838 kb on - strand, within Pf1N1B4_3466 at 3873.934 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.011 kb on + strand at 3874.012 kb on - strand at 3874.012 kb on - strand at 3874.012 kb on - strand at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.135 kb on + strand, within Pf1N1B4_3467 at 3874.136 kb on - strand, within Pf1N1B4_3467 at 3874.136 kb on - strand, within Pf1N1B4_3467 at 3874.220 kb on + strand, within Pf1N1B4_3467 at 3874.527 kb on + strand at 3874.527 kb on + strand at 3874.532 kb on + strand at 3874.532 kb on + strand at 3874.533 kb on - strand at 3874.649 kb on - strand, within Pf1N1B4_3468 at 3874.650 kb on + strand, within Pf1N1B4_3468 at 3874.650 kb on + strand, within Pf1N1B4_3468 at 3874.861 kb on + strand, within Pf1N1B4_3468 at 3874.936 kb on + strand at 3874.991 kb on - strand at 3875.089 kb on + strand at 3875.089 kb on + strand at 3875.089 kb on + strand at 3875.089 kb on + strand at 3875.090 kb on - strand at 3875.090 kb on - strand at 3875.297 kb on + strand, within Pf1N1B4_3469 at 3875.297 kb on + strand, within Pf1N1B4_3469 at 3875.297 kb on + strand, within Pf1N1B4_3469 at 3875.297 kb on + strand, within Pf1N1B4_3469 at 3875.298 kb on - strand, within Pf1N1B4_3469 at 3875.298 kb on - strand, within Pf1N1B4_3469 at 3875.407 kb on - strand, within Pf1N1B4_3469 at 3875.478 kb on + strand, within Pf1N1B4_3469 at 3875.478 kb on + strand, within Pf1N1B4_3469 at 3875.478 kb on + strand, within Pf1N1B4_3469 at 3875.478 kb on + strand, within Pf1N1B4_3469 at 3875.479 kb on - strand, within Pf1N1B4_3469 at 3875.479 kb on - strand, within Pf1N1B4_3469 at 3875.490 kb on + strand, within Pf1N1B4_3469
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,873,240 + -0.9 3,873,288 + +0.9 3,873,288 + +0.9 3,873,289 - +0.3 3,873,289 - -0.3 3,873,289 - -0.7 3,873,385 - Pf1N1B4_3466 0.19 -2.3 3,873,624 + +0.9 3,873,625 - Pf1N1B4_3466 0.51 +0.9 3,873,654 + Pf1N1B4_3466 0.55 -1.1 3,873,716 + Pf1N1B4_3466 0.63 -0.7 3,873,837 + Pf1N1B4_3466 0.80 -0.0 3,873,838 - Pf1N1B4_3466 0.80 +1.1 3,873,934 + -0.1 3,874,011 + +0.3 3,874,011 + +1.4 3,874,011 + +0.5 3,874,011 + +0.3 3,874,011 + +0.6 3,874,011 + +0.5 3,874,011 + +1.9 3,874,011 + -1.1 3,874,011 + +0.1 3,874,011 + -0.2 3,874,011 + +2.0 3,874,011 + -2.4 3,874,011 + -0.5 3,874,011 + +0.9 3,874,011 + -0.6 3,874,011 + +0.5 3,874,011 + +0.2 3,874,011 + +0.5 3,874,011 + +0.6 3,874,011 + +0.5 3,874,011 + +0.1 3,874,012 - -0.1 3,874,012 - +0.1 3,874,012 - +2.5 3,874,135 + Pf1N1B4_3467 0.12 +0.3 3,874,135 + Pf1N1B4_3467 0.12 -0.4 3,874,135 + Pf1N1B4_3467 0.12 -1.7 3,874,135 + Pf1N1B4_3467 0.12 -0.6 3,874,136 - Pf1N1B4_3467 0.13 +0.1 3,874,136 - Pf1N1B4_3467 0.13 -2.3 3,874,220 + Pf1N1B4_3467 0.31 -0.3 3,874,527 + +0.2 3,874,527 + -0.6 3,874,532 + -1.7 3,874,532 + -0.5 3,874,533 - +0.1 3,874,649 - Pf1N1B4_3468 0.17 -0.1 3,874,650 + Pf1N1B4_3468 0.18 -1.9 3,874,650 + Pf1N1B4_3468 0.18 -0.5 3,874,861 + Pf1N1B4_3468 0.82 -0.5 3,874,936 + -1.1 3,874,991 - -0.7 3,875,089 + -0.1 3,875,089 + -1.7 3,875,089 + -1.1 3,875,089 + +0.1 3,875,090 - +0.9 3,875,090 - -0.7 3,875,297 + Pf1N1B4_3469 0.30 -0.1 3,875,297 + Pf1N1B4_3469 0.30 +0.7 3,875,297 + Pf1N1B4_3469 0.30 -0.5 3,875,297 + Pf1N1B4_3469 0.30 +0.5 3,875,298 - Pf1N1B4_3469 0.30 +1.3 3,875,298 - Pf1N1B4_3469 0.30 -0.7 3,875,407 - Pf1N1B4_3469 0.46 +2.5 3,875,478 + Pf1N1B4_3469 0.56 -0.5 3,875,478 + Pf1N1B4_3469 0.56 +1.4 3,875,478 + Pf1N1B4_3469 0.56 +0.0 3,875,478 + Pf1N1B4_3469 0.56 +1.3 3,875,479 - Pf1N1B4_3469 0.56 +0.5 3,875,479 - Pf1N1B4_3469 0.56 +0.1 3,875,490 + Pf1N1B4_3469 0.58 +0.9
Or see this region's nucleotide sequence