Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_343 and Pf1N1B4_344 are separated by 226 nucleotides Pf1N1B4_344 and Pf1N1B4_345 are separated by 27 nucleotides Pf1N1B4_345 and Pf1N1B4_346 are separated by 645 nucleotides
Pf1N1B4_343 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 406,870 to 407,946
_343
Pf1N1B4_344 - hypothetical protein, at 408,173 to 408,412
_344
Pf1N1B4_345 - Integration host factor beta subunit, at 408,440 to 408,736
_345
Pf1N1B4_346 - SSU ribosomal protein S1p, at 409,382 to 411,073
_346
Position (kb) 408
409 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 407.472 kb on - strand, within Pf1N1B4_343 at 407.557 kb on - strand, within Pf1N1B4_343 at 407.557 kb on - strand, within Pf1N1B4_343 at 407.841 kb on - strand at 407.963 kb on - strand at 408.095 kb on - strand at 408.101 kb on + strand at 408.102 kb on - strand at 408.113 kb on + strand at 408.113 kb on + strand at 408.114 kb on - strand at 408.341 kb on + strand, within Pf1N1B4_344 at 408.380 kb on + strand, within Pf1N1B4_344 at 408.380 kb on + strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.392 kb on + strand at 408.392 kb on + strand at 408.393 kb on - strand at 408.393 kb on - strand at 408.393 kb on - strand at 408.574 kb on - strand, within Pf1N1B4_345 at 408.825 kb on + strand at 408.825 kb on + strand at 408.825 kb on + strand at 408.826 kb on - strand at 408.826 kb on - strand at 408.892 kb on + strand at 408.893 kb on - strand at 408.950 kb on + strand at 408.950 kb on + strand at 408.950 kb on + strand at 408.950 kb on + strand at 408.951 kb on - strand at 408.992 kb on - strand at 408.992 kb on - strand at 409.074 kb on - strand at 409.136 kb on + strand at 409.137 kb on - strand at 409.175 kb on + strand at 409.176 kb on - strand at 409.214 kb on + strand at 409.230 kb on + strand at 409.230 kb on + strand at 409.230 kb on + strand at 409.230 kb on + strand at 409.230 kb on + strand at 409.268 kb on + strand at 409.268 kb on + strand at 409.268 kb on + strand at 409.268 kb on + strand at 409.269 kb on - strand at 409.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 407,472 - Pf1N1B4_343 0.56 -1.5 407,557 - Pf1N1B4_343 0.64 +1.1 407,557 - Pf1N1B4_343 0.64 -0.3 407,841 - -0.1 407,963 - -0.6 408,095 - -1.9 408,101 + +0.9 408,102 - +0.9 408,113 + +0.3 408,113 + -1.9 408,114 - +0.9 408,341 + Pf1N1B4_344 0.70 -1.1 408,380 + Pf1N1B4_344 0.86 +0.1 408,380 + Pf1N1B4_344 0.86 +0.1 408,381 - Pf1N1B4_344 0.87 -0.4 408,381 - Pf1N1B4_344 0.87 +0.2 408,381 - Pf1N1B4_344 0.87 +1.4 408,381 - -0.1 408,381 - Pf1N1B4_344 0.87 +1.5 408,381 - Pf1N1B4_344 0.87 +0.3 408,381 - Pf1N1B4_344 0.87 +0.9 408,381 - Pf1N1B4_344 0.87 +1.4 408,392 + -0.1 408,392 + +0.2 408,393 - -0.5 408,393 - +0.9 408,393 - +1.3 408,574 - Pf1N1B4_345 0.45 -1.1 408,825 + -1.6 408,825 + +0.4 408,825 + -0.6 408,826 - -0.5 408,826 - +1.2 408,892 + -0.7 408,893 - -0.3 408,950 + +0.1 408,950 + -0.5 408,950 + -0.8 408,950 + +0.9 408,951 - -0.7 408,992 - +0.9 408,992 - +0.1 409,074 - -0.4 409,136 + +1.4 409,137 - +1.4 409,175 + -0.1 409,176 - +2.4 409,214 + -1.5 409,230 + -0.7 409,230 + +2.9 409,230 + +1.3 409,230 + -1.7 409,230 + +2.1 409,268 + +0.9 409,268 + +1.3 409,268 + -1.4 409,268 + +0.3 409,269 - +0.9 409,269 - +0.1
Or see this region's nucleotide sequence