Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3448 and Pf1N1B4_3449 are separated by 573 nucleotides Pf1N1B4_3449 and Pf1N1B4_3450 are separated by 146 nucleotides
Pf1N1B4_3448 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 3,843,591 to 3,844,100
_3448
Pf1N1B4_3449 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 3,844,674 to 3,845,678
_3449
Pf1N1B4_3450 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 3,845,825 to 3,848,446
_3450
Position (kb)
3844
3845
3846 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3843.680 kb on + strand at 3843.680 kb on + strand, within Pf1N1B4_3448 at 3843.681 kb on - strand, within Pf1N1B4_3448 at 3843.681 kb on - strand at 3843.832 kb on + strand, within Pf1N1B4_3448 at 3843.832 kb on + strand, within Pf1N1B4_3448 at 3843.833 kb on - strand, within Pf1N1B4_3448 at 3843.833 kb on - strand, within Pf1N1B4_3448 at 3843.833 kb on - strand, within Pf1N1B4_3448 at 3843.833 kb on - strand, within Pf1N1B4_3448 at 3844.154 kb on - strand at 3844.159 kb on + strand at 3844.194 kb on + strand at 3844.194 kb on + strand at 3844.196 kb on + strand at 3844.196 kb on + strand at 3844.196 kb on + strand at 3844.239 kb on + strand at 3844.240 kb on - strand at 3844.240 kb on - strand at 3844.240 kb on - strand at 3844.240 kb on - strand at 3844.327 kb on + strand at 3844.417 kb on + strand at 3844.418 kb on - strand at 3844.424 kb on + strand at 3844.424 kb on + strand at 3844.424 kb on + strand at 3844.424 kb on + strand at 3844.425 kb on - strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.507 kb on + strand at 3844.508 kb on - strand at 3844.606 kb on + strand at 3844.627 kb on + strand at 3844.628 kb on - strand at 3844.767 kb on + strand at 3844.768 kb on - strand at 3844.768 kb on - strand at 3844.768 kb on - strand at 3844.768 kb on - strand at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.905 kb on + strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.906 kb on - strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.932 kb on + strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.933 kb on - strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.934 kb on + strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.935 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3844.939 kb on - strand, within Pf1N1B4_3449 at 3845.122 kb on - strand, within Pf1N1B4_3449 at 3845.122 kb on - strand, within Pf1N1B4_3449 at 3845.190 kb on + strand, within Pf1N1B4_3449 at 3845.190 kb on + strand, within Pf1N1B4_3449 at 3845.191 kb on - strand, within Pf1N1B4_3449 at 3845.191 kb on - strand, within Pf1N1B4_3449 at 3845.397 kb on + strand, within Pf1N1B4_3449 at 3845.398 kb on - strand, within Pf1N1B4_3449 at 3845.398 kb on - strand, within Pf1N1B4_3449 at 3845.514 kb on + strand, within Pf1N1B4_3449 at 3845.514 kb on + strand, within Pf1N1B4_3449 at 3845.514 kb on + strand, within Pf1N1B4_3449 at 3845.514 kb on + strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.515 kb on - strand at 3845.515 kb on - strand, within Pf1N1B4_3449 at 3845.707 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.725 kb on + strand at 3845.729 kb on + strand at 3845.729 kb on + strand at 3845.729 kb on + strand at 3845.729 kb on + strand at 3845.729 kb on + strand at 3845.745 kb on + strand at 3845.745 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand at 3845.747 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,843,680 + +0.3 3,843,680 + Pf1N1B4_3448 0.17 +0.9 3,843,681 - Pf1N1B4_3448 0.18 -0.1 3,843,681 - -0.1 3,843,832 + Pf1N1B4_3448 0.47 +0.7 3,843,832 + Pf1N1B4_3448 0.47 -0.3 3,843,833 - Pf1N1B4_3448 0.47 -0.4 3,843,833 - Pf1N1B4_3448 0.47 +1.2 3,843,833 - Pf1N1B4_3448 0.47 +0.6 3,843,833 - Pf1N1B4_3448 0.47 +2.2 3,844,154 - -0.1 3,844,159 + +1.6 3,844,194 + -0.1 3,844,194 + +1.9 3,844,196 + -0.5 3,844,196 + -0.4 3,844,196 + -0.3 3,844,239 + +0.7 3,844,240 - -1.1 3,844,240 - -0.3 3,844,240 - +1.9 3,844,240 - -0.1 3,844,327 + -0.7 3,844,417 + -0.4 3,844,418 - -1.4 3,844,424 + -0.0 3,844,424 + -1.4 3,844,424 + +0.9 3,844,424 + -1.4 3,844,425 - +0.7 3,844,507 + -0.7 3,844,507 + +1.1 3,844,507 + -1.1 3,844,507 + -1.4 3,844,507 + -0.7 3,844,507 + -0.9 3,844,507 + -0.1 3,844,508 - -0.4 3,844,606 + -1.7 3,844,627 + +0.9 3,844,628 - -1.1 3,844,767 + +1.8 3,844,768 - -0.7 3,844,768 - -0.7 3,844,768 - +0.1 3,844,768 - -0.1 3,844,905 + Pf1N1B4_3449 0.23 -0.1 3,844,905 + Pf1N1B4_3449 0.23 -0.8 3,844,905 + Pf1N1B4_3449 0.23 +0.1 3,844,905 + Pf1N1B4_3449 0.23 +2.3 3,844,905 + Pf1N1B4_3449 0.23 -0.3 3,844,906 - Pf1N1B4_3449 0.23 +1.6 3,844,906 - Pf1N1B4_3449 0.23 -0.1 3,844,906 - Pf1N1B4_3449 0.23 +3.7 3,844,906 - Pf1N1B4_3449 0.23 -1.1 3,844,906 - Pf1N1B4_3449 0.23 -0.4 3,844,906 - Pf1N1B4_3449 0.23 -0.1 3,844,906 - Pf1N1B4_3449 0.23 -0.6 3,844,932 + Pf1N1B4_3449 0.26 +0.6 3,844,932 + Pf1N1B4_3449 0.26 +1.0 3,844,932 + +1.9 3,844,932 + Pf1N1B4_3449 0.26 -0.4 3,844,932 + Pf1N1B4_3449 0.26 +1.3 3,844,932 + Pf1N1B4_3449 0.26 +0.9 3,844,932 + Pf1N1B4_3449 0.26 +1.3 3,844,933 - Pf1N1B4_3449 0.26 +0.6 3,844,933 - Pf1N1B4_3449 0.26 +1.9 3,844,933 - +0.3 3,844,933 - Pf1N1B4_3449 0.26 -0.9 3,844,933 - Pf1N1B4_3449 0.26 +1.6 3,844,933 - Pf1N1B4_3449 0.26 -0.1 3,844,933 - Pf1N1B4_3449 0.26 +1.3 3,844,933 - Pf1N1B4_3449 0.26 -0.7 3,844,933 - Pf1N1B4_3449 0.26 -1.1 3,844,933 - Pf1N1B4_3449 0.26 -0.1 3,844,933 - Pf1N1B4_3449 0.26 -0.8 3,844,933 - Pf1N1B4_3449 0.26 -1.4 3,844,934 + Pf1N1B4_3449 0.26 +0.3 3,844,934 + Pf1N1B4_3449 0.26 +0.2 3,844,934 + Pf1N1B4_3449 0.26 +0.1 3,844,934 + Pf1N1B4_3449 0.26 +0.5 3,844,934 + Pf1N1B4_3449 0.26 +0.3 3,844,934 + Pf1N1B4_3449 0.26 +0.4 3,844,934 + Pf1N1B4_3449 0.26 -1.4 3,844,935 - Pf1N1B4_3449 0.26 +0.2 3,844,935 - Pf1N1B4_3449 0.26 +1.3 3,844,935 - Pf1N1B4_3449 0.26 -0.5 3,844,935 - Pf1N1B4_3449 0.26 -0.1 3,844,935 - Pf1N1B4_3449 0.26 +2.2 3,844,935 - Pf1N1B4_3449 0.26 -0.5 3,844,935 - Pf1N1B4_3449 0.26 -0.3 3,844,939 - -0.7 3,844,939 - Pf1N1B4_3449 0.26 +0.3 3,844,939 - Pf1N1B4_3449 0.26 +0.4 3,844,939 - Pf1N1B4_3449 0.26 +0.2 3,844,939 - Pf1N1B4_3449 0.26 +0.0 3,844,939 - Pf1N1B4_3449 0.26 +0.8 3,844,939 - Pf1N1B4_3449 0.26 -1.3 3,844,939 - Pf1N1B4_3449 0.26 -0.1 3,845,122 - Pf1N1B4_3449 0.45 +1.1 3,845,122 - Pf1N1B4_3449 0.45 +0.2 3,845,190 + Pf1N1B4_3449 0.51 +0.7 3,845,190 + Pf1N1B4_3449 0.51 +0.1 3,845,191 - Pf1N1B4_3449 0.51 +0.0 3,845,191 - Pf1N1B4_3449 0.51 +2.5 3,845,397 + Pf1N1B4_3449 0.72 +0.9 3,845,398 - Pf1N1B4_3449 0.72 +0.6 3,845,398 - Pf1N1B4_3449 0.72 +3.2 3,845,514 + Pf1N1B4_3449 0.84 -0.3 3,845,514 + Pf1N1B4_3449 0.84 +1.1 3,845,514 + Pf1N1B4_3449 0.84 +0.5 3,845,514 + Pf1N1B4_3449 0.84 +0.5 3,845,515 - Pf1N1B4_3449 0.84 -0.4 3,845,515 - Pf1N1B4_3449 0.84 -1.7 3,845,515 - Pf1N1B4_3449 0.84 +0.4 3,845,515 - Pf1N1B4_3449 0.84 -0.1 3,845,515 - Pf1N1B4_3449 0.84 -1.4 3,845,515 - -0.7 3,845,515 - Pf1N1B4_3449 0.84 -0.2 3,845,707 + +0.2 3,845,725 + +1.9 3,845,725 + -0.7 3,845,725 + +0.9 3,845,725 + -1.4 3,845,725 + -2.4 3,845,725 + +1.4 3,845,729 + -0.3 3,845,729 + -0.7 3,845,729 + -0.1 3,845,729 + +1.1 3,845,729 + -1.9 3,845,745 + -1.0 3,845,745 + +0.3 3,845,747 + -2.6 3,845,747 + -0.3 3,845,747 + -1.1 3,845,747 + -1.9 3,845,747 + -1.9 3,845,747 + -0.1 3,845,747 + -1.1
Or see this region's nucleotide sequence