Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_341 and Pf1N1B4_342 overlap by 4 nucleotides Pf1N1B4_342 and Pf1N1B4_343 overlap by 4 nucleotides Pf1N1B4_343 and Pf1N1B4_344 are separated by 226 nucleotides Pf1N1B4_344 and Pf1N1B4_345 are separated by 27 nucleotides
Pf1N1B4_341 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 405,093 to 405,983
_341
Pf1N1B4_342 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 405,980 to 406,873
_342
Pf1N1B4_343 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 406,870 to 407,946
_343
Pf1N1B4_344 - hypothetical protein, at 408,173 to 408,412
_344
Pf1N1B4_345 - Integration host factor beta subunit, at 408,440 to 408,736
_345
Position (kb) 406
407
408 Strain fitness (log2 ratio) -2
-1
0
1
2 at 405.966 kb on - strand at 405.985 kb on + strand at 405.985 kb on + strand at 406.896 kb on - strand at 406.943 kb on - strand at 406.943 kb on - strand at 407.055 kb on - strand, within Pf1N1B4_343 at 407.088 kb on - strand at 407.088 kb on - strand, within Pf1N1B4_343 at 407.158 kb on - strand, within Pf1N1B4_343 at 407.205 kb on - strand, within Pf1N1B4_343 at 407.205 kb on - strand, within Pf1N1B4_343 at 407.205 kb on - strand, within Pf1N1B4_343 at 407.205 kb on - strand, within Pf1N1B4_343 at 407.241 kb on - strand, within Pf1N1B4_343 at 407.241 kb on - strand, within Pf1N1B4_343 at 407.241 kb on - strand, within Pf1N1B4_343 at 407.241 kb on - strand, within Pf1N1B4_343 at 407.268 kb on - strand, within Pf1N1B4_343 at 407.268 kb on - strand, within Pf1N1B4_343 at 407.418 kb on - strand, within Pf1N1B4_343 at 407.418 kb on - strand, within Pf1N1B4_343 at 407.422 kb on - strand, within Pf1N1B4_343 at 407.472 kb on - strand, within Pf1N1B4_343 at 407.557 kb on - strand, within Pf1N1B4_343 at 407.557 kb on - strand, within Pf1N1B4_343 at 407.841 kb on - strand at 407.963 kb on - strand at 408.095 kb on - strand at 408.101 kb on + strand at 408.102 kb on - strand at 408.113 kb on + strand at 408.113 kb on + strand at 408.114 kb on - strand at 408.341 kb on + strand, within Pf1N1B4_344 at 408.380 kb on + strand, within Pf1N1B4_344 at 408.380 kb on + strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.392 kb on + strand at 408.392 kb on + strand at 408.393 kb on - strand at 408.393 kb on - strand at 408.393 kb on - strand at 408.574 kb on - strand, within Pf1N1B4_345 at 408.825 kb on + strand at 408.825 kb on + strand at 408.825 kb on + strand at 408.826 kb on - strand at 408.826 kb on - strand at 408.892 kb on + strand at 408.893 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 405,966 - +0.9 405,985 + +0.9 405,985 + +1.1 406,896 - +0.6 406,943 - -1.1 406,943 - +0.3 407,055 - Pf1N1B4_343 0.17 -1.5 407,088 - -0.7 407,088 - Pf1N1B4_343 0.20 +2.2 407,158 - Pf1N1B4_343 0.27 +1.1 407,205 - Pf1N1B4_343 0.31 +1.4 407,205 - Pf1N1B4_343 0.31 +0.4 407,205 - Pf1N1B4_343 0.31 +1.4 407,205 - Pf1N1B4_343 0.31 -0.5 407,241 - Pf1N1B4_343 0.34 -1.9 407,241 - Pf1N1B4_343 0.34 +0.9 407,241 - Pf1N1B4_343 0.34 +0.3 407,241 - Pf1N1B4_343 0.34 +0.9 407,268 - Pf1N1B4_343 0.37 -1.7 407,268 - Pf1N1B4_343 0.37 -1.5 407,418 - Pf1N1B4_343 0.51 +1.7 407,418 - Pf1N1B4_343 0.51 -0.1 407,422 - Pf1N1B4_343 0.51 +1.4 407,472 - Pf1N1B4_343 0.56 -1.5 407,557 - Pf1N1B4_343 0.64 +1.1 407,557 - Pf1N1B4_343 0.64 -0.3 407,841 - -0.1 407,963 - -0.6 408,095 - -1.9 408,101 + +0.9 408,102 - +0.9 408,113 + +0.3 408,113 + -1.9 408,114 - +0.9 408,341 + Pf1N1B4_344 0.70 -1.1 408,380 + Pf1N1B4_344 0.86 +0.1 408,380 + Pf1N1B4_344 0.86 +0.1 408,381 - Pf1N1B4_344 0.87 -0.4 408,381 - Pf1N1B4_344 0.87 +0.2 408,381 - Pf1N1B4_344 0.87 +1.4 408,381 - -0.1 408,381 - Pf1N1B4_344 0.87 +1.5 408,381 - Pf1N1B4_344 0.87 +0.3 408,381 - Pf1N1B4_344 0.87 +0.9 408,381 - Pf1N1B4_344 0.87 +1.4 408,392 + -0.1 408,392 + +0.2 408,393 - -0.5 408,393 - +0.9 408,393 - +1.3 408,574 - Pf1N1B4_345 0.45 -1.1 408,825 + -1.6 408,825 + +0.4 408,825 + -0.6 408,826 - -0.5 408,826 - +1.2 408,892 + -0.7 408,893 - -0.3
Or see this region's nucleotide sequence