Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3345 and Pf1N1B4_3346 overlap by 4 nucleotides Pf1N1B4_3346 and Pf1N1B4_3347 are separated by 120 nucleotides Pf1N1B4_3347 and Pf1N1B4_3348 are separated by 81 nucleotides Pf1N1B4_3348 and Pf1N1B4_3349 are separated by 277 nucleotides
Pf1N1B4_3345 - Ribonuclease D (EC 3.1.26.3), at 3,733,323 to 3,734,456
_3345
Pf1N1B4_3346 - Protein YcgL, at 3,734,453 to 3,734,746
_3346
Pf1N1B4_3347 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,734,867 to 3,735,799
_3347
Pf1N1B4_3348 - Nitroreductase family protein, at 3,735,881 to 3,736,474
_3348
Pf1N1B4_3349 - YcgN (Fragment), at 3,736,752 to 3,737,201
_3349
Position (kb)
3734
3735
3736 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3733.915 kb on - strand, within Pf1N1B4_3345 at 3733.915 kb on - strand, within Pf1N1B4_3345 at 3733.923 kb on + strand, within Pf1N1B4_3345 at 3733.924 kb on - strand, within Pf1N1B4_3345 at 3733.973 kb on - strand, within Pf1N1B4_3345 at 3734.107 kb on + strand, within Pf1N1B4_3345 at 3734.107 kb on + strand, within Pf1N1B4_3345 at 3734.107 kb on + strand, within Pf1N1B4_3345 at 3734.107 kb on + strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.108 kb on - strand, within Pf1N1B4_3345 at 3734.462 kb on - strand at 3734.462 kb on - strand at 3734.462 kb on - strand at 3734.462 kb on - strand at 3734.462 kb on - strand at 3734.475 kb on - strand at 3734.475 kb on - strand at 3734.475 kb on - strand at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.502 kb on - strand, within Pf1N1B4_3346 at 3734.507 kb on + strand, within Pf1N1B4_3346 at 3734.507 kb on + strand, within Pf1N1B4_3346 at 3734.507 kb on + strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.508 kb on - strand, within Pf1N1B4_3346 at 3734.697 kb on - strand, within Pf1N1B4_3346 at 3734.697 kb on - strand, within Pf1N1B4_3346 at 3734.745 kb on - strand at 3734.822 kb on + strand at 3734.903 kb on + strand at 3734.903 kb on + strand at 3734.904 kb on - strand at 3734.904 kb on - strand at 3734.943 kb on + strand at 3735.164 kb on + strand, within Pf1N1B4_3347 at 3735.164 kb on + strand, within Pf1N1B4_3347 at 3735.164 kb on + strand, within Pf1N1B4_3347 at 3735.164 kb on + strand at 3735.165 kb on - strand, within Pf1N1B4_3347 at 3735.165 kb on - strand, within Pf1N1B4_3347 at 3735.168 kb on + strand, within Pf1N1B4_3347 at 3735.168 kb on + strand, within Pf1N1B4_3347 at 3735.169 kb on - strand at 3735.169 kb on - strand, within Pf1N1B4_3347 at 3735.176 kb on + strand at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.176 kb on + strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.177 kb on - strand, within Pf1N1B4_3347 at 3735.183 kb on + strand, within Pf1N1B4_3347 at 3735.184 kb on - strand, within Pf1N1B4_3347 at 3735.348 kb on - strand, within Pf1N1B4_3347 at 3735.437 kb on + strand, within Pf1N1B4_3347 at 3735.437 kb on + strand, within Pf1N1B4_3347 at 3735.438 kb on - strand, within Pf1N1B4_3347 at 3735.438 kb on - strand, within Pf1N1B4_3347 at 3735.482 kb on + strand, within Pf1N1B4_3347 at 3736.302 kb on + strand, within Pf1N1B4_3348 at 3736.329 kb on + strand, within Pf1N1B4_3348 at 3736.329 kb on + strand, within Pf1N1B4_3348 at 3736.447 kb on - strand at 3736.465 kb on + strand at 3736.489 kb on - strand at 3736.564 kb on + strand at 3736.564 kb on + strand at 3736.633 kb on + strand at 3736.633 kb on + strand at 3736.633 kb on + strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.634 kb on - strand at 3736.646 kb on + strand at 3736.646 kb on + strand at 3736.648 kb on + strand at 3736.648 kb on + strand at 3736.648 kb on + strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.649 kb on - strand at 3736.706 kb on + strand at 3736.709 kb on + strand at 3736.709 kb on + strand at 3736.709 kb on + strand at 3736.710 kb on - strand at 3736.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,733,915 - Pf1N1B4_3345 0.52 +0.5 3,733,915 - Pf1N1B4_3345 0.52 +0.9 3,733,923 + Pf1N1B4_3345 0.53 -0.3 3,733,924 - Pf1N1B4_3345 0.53 +0.1 3,733,973 - Pf1N1B4_3345 0.57 +0.8 3,734,107 + Pf1N1B4_3345 0.69 +0.2 3,734,107 + Pf1N1B4_3345 0.69 -1.3 3,734,107 + Pf1N1B4_3345 0.69 -1.4 3,734,107 + Pf1N1B4_3345 0.69 +0.7 3,734,108 - Pf1N1B4_3345 0.69 +2.1 3,734,108 - Pf1N1B4_3345 0.69 +0.1 3,734,108 - Pf1N1B4_3345 0.69 -1.4 3,734,108 - Pf1N1B4_3345 0.69 -0.4 3,734,108 - Pf1N1B4_3345 0.69 +1.2 3,734,462 - -0.1 3,734,462 - +0.4 3,734,462 - -0.1 3,734,462 - +0.4 3,734,462 - +0.8 3,734,475 - +0.3 3,734,475 - -1.1 3,734,475 - +1.1 3,734,502 - Pf1N1B4_3346 0.17 +1.1 3,734,502 - Pf1N1B4_3346 0.17 +0.0 3,734,502 - Pf1N1B4_3346 0.17 +0.9 3,734,502 - Pf1N1B4_3346 0.17 -0.5 3,734,502 - Pf1N1B4_3346 0.17 -0.1 3,734,502 - Pf1N1B4_3346 0.17 -1.1 3,734,507 + Pf1N1B4_3346 0.18 -0.4 3,734,507 + Pf1N1B4_3346 0.18 -0.1 3,734,507 + Pf1N1B4_3346 0.18 -1.8 3,734,508 - Pf1N1B4_3346 0.19 -1.9 3,734,508 - Pf1N1B4_3346 0.19 -0.7 3,734,508 - Pf1N1B4_3346 0.19 +0.9 3,734,508 - Pf1N1B4_3346 0.19 +0.6 3,734,508 - Pf1N1B4_3346 0.19 +0.7 3,734,508 - Pf1N1B4_3346 0.19 +1.1 3,734,508 - Pf1N1B4_3346 0.19 +0.3 3,734,508 - Pf1N1B4_3346 0.19 -1.9 3,734,508 - Pf1N1B4_3346 0.19 +0.5 3,734,508 - Pf1N1B4_3346 0.19 +0.5 3,734,508 - Pf1N1B4_3346 0.19 -1.1 3,734,697 - Pf1N1B4_3346 0.83 +0.2 3,734,697 - Pf1N1B4_3346 0.83 -0.5 3,734,745 - -0.1 3,734,822 + +0.2 3,734,903 + -0.1 3,734,903 + -1.2 3,734,904 - +0.9 3,734,904 - -0.7 3,734,943 + +0.9 3,735,164 + Pf1N1B4_3347 0.32 +0.4 3,735,164 + Pf1N1B4_3347 0.32 +0.3 3,735,164 + Pf1N1B4_3347 0.32 +0.9 3,735,164 + -1.1 3,735,165 - Pf1N1B4_3347 0.32 +1.1 3,735,165 - Pf1N1B4_3347 0.32 +1.9 3,735,168 + Pf1N1B4_3347 0.32 +0.9 3,735,168 + Pf1N1B4_3347 0.32 +1.5 3,735,169 - +2.9 3,735,169 - Pf1N1B4_3347 0.32 +0.2 3,735,176 + -0.1 3,735,176 + Pf1N1B4_3347 0.33 -0.1 3,735,176 + Pf1N1B4_3347 0.33 -0.4 3,735,176 + Pf1N1B4_3347 0.33 +0.3 3,735,176 + Pf1N1B4_3347 0.33 +0.9 3,735,176 + Pf1N1B4_3347 0.33 +1.8 3,735,176 + Pf1N1B4_3347 0.33 +0.5 3,735,176 + Pf1N1B4_3347 0.33 -1.2 3,735,176 + Pf1N1B4_3347 0.33 -0.7 3,735,176 + Pf1N1B4_3347 0.33 +2.5 3,735,176 + Pf1N1B4_3347 0.33 +1.2 3,735,177 - Pf1N1B4_3347 0.33 +0.9 3,735,177 - Pf1N1B4_3347 0.33 -1.4 3,735,177 - +2.5 3,735,177 - Pf1N1B4_3347 0.33 +1.1 3,735,177 - Pf1N1B4_3347 0.33 -0.7 3,735,177 - Pf1N1B4_3347 0.33 +0.9 3,735,177 - Pf1N1B4_3347 0.33 -0.4 3,735,177 - Pf1N1B4_3347 0.33 -0.1 3,735,177 - Pf1N1B4_3347 0.33 +0.6 3,735,177 - +0.9 3,735,177 - Pf1N1B4_3347 0.33 -0.1 3,735,177 - Pf1N1B4_3347 0.33 +0.9 3,735,177 - Pf1N1B4_3347 0.33 -0.8 3,735,183 + Pf1N1B4_3347 0.34 -0.0 3,735,184 - Pf1N1B4_3347 0.34 +0.1 3,735,348 - Pf1N1B4_3347 0.52 +2.5 3,735,437 + Pf1N1B4_3347 0.61 +1.2 3,735,437 + Pf1N1B4_3347 0.61 -0.3 3,735,438 - Pf1N1B4_3347 0.61 +1.1 3,735,438 - Pf1N1B4_3347 0.61 -0.8 3,735,482 + Pf1N1B4_3347 0.66 +0.7 3,736,302 + Pf1N1B4_3348 0.71 +0.3 3,736,329 + Pf1N1B4_3348 0.75 +0.1 3,736,329 + Pf1N1B4_3348 0.75 +0.1 3,736,447 - +1.7 3,736,465 + -0.1 3,736,489 - +0.9 3,736,564 + -1.4 3,736,564 + -0.7 3,736,633 + -1.9 3,736,633 + +0.9 3,736,633 + +0.2 3,736,634 - +0.9 3,736,634 - -1.4 3,736,634 - -0.7 3,736,634 - -0.9 3,736,646 + +0.4 3,736,646 + +1.3 3,736,648 + +0.2 3,736,648 + -0.1 3,736,648 + -0.7 3,736,649 - +1.3 3,736,649 - -1.4 3,736,649 - -1.2 3,736,649 - +0.4 3,736,649 - +0.5 3,736,706 + -1.3 3,736,709 + -1.9 3,736,709 + -2.1 3,736,709 + -1.1 3,736,710 - -0.7 3,736,710 - -0.9
Or see this region's nucleotide sequence