Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3311 and Pf1N1B4_3312 are separated by 1 nucleotides Pf1N1B4_3312 and Pf1N1B4_3313 are separated by 58 nucleotides
Pf1N1B4_3311 - Tricarboxylate transport membrane protein TctA, at 3,699,961 to 3,701,475
_3311
Pf1N1B4_3312 - Tricarboxylate transport protein TctB, at 3,701,477 to 3,701,935
_3312
Pf1N1B4_3313 - Tricarboxylate transport protein TctC, at 3,701,994 to 3,702,971
_3313
Position (kb)
3701
3702 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3700.617 kb on + strand, within Pf1N1B4_3311 at 3700.618 kb on - strand, within Pf1N1B4_3311 at 3700.618 kb on - strand, within Pf1N1B4_3311 at 3700.618 kb on - strand, within Pf1N1B4_3311 at 3700.634 kb on + strand at 3700.634 kb on + strand, within Pf1N1B4_3311 at 3700.635 kb on - strand, within Pf1N1B4_3311 at 3700.696 kb on + strand, within Pf1N1B4_3311 at 3700.881 kb on + strand, within Pf1N1B4_3311 at 3700.882 kb on - strand, within Pf1N1B4_3311 at 3700.898 kb on + strand, within Pf1N1B4_3311 at 3700.898 kb on + strand, within Pf1N1B4_3311 at 3700.899 kb on - strand, within Pf1N1B4_3311 at 3701.031 kb on - strand, within Pf1N1B4_3311 at 3701.031 kb on - strand, within Pf1N1B4_3311 at 3701.198 kb on - strand, within Pf1N1B4_3311 at 3701.253 kb on - strand, within Pf1N1B4_3311 at 3701.268 kb on - strand, within Pf1N1B4_3311 at 3701.268 kb on - strand, within Pf1N1B4_3311 at 3701.459 kb on + strand at 3701.460 kb on - strand at 3701.469 kb on + strand at 3701.469 kb on + strand at 3701.470 kb on - strand at 3701.475 kb on + strand at 3701.545 kb on - strand, within Pf1N1B4_3312 at 3701.578 kb on + strand, within Pf1N1B4_3312 at 3701.602 kb on - strand, within Pf1N1B4_3312 at 3701.778 kb on + strand, within Pf1N1B4_3312 at 3701.779 kb on - strand, within Pf1N1B4_3312 at 3701.779 kb on - strand, within Pf1N1B4_3312 at 3701.779 kb on - strand, within Pf1N1B4_3312 at 3701.779 kb on - strand at 3701.779 kb on - strand, within Pf1N1B4_3312 at 3701.779 kb on - strand, within Pf1N1B4_3312 at 3701.810 kb on + strand, within Pf1N1B4_3312 at 3701.927 kb on + strand at 3701.927 kb on + strand at 3701.927 kb on + strand at 3701.927 kb on + strand at 3701.927 kb on + strand at 3701.927 kb on + strand at 3701.927 kb on + strand at 3701.927 kb on + strand at 3701.927 kb on + strand at 3701.927 kb on + strand at 3701.928 kb on - strand at 3701.928 kb on - strand at 3701.928 kb on - strand at 3701.928 kb on - strand at 3701.928 kb on - strand at 3701.928 kb on - strand at 3701.928 kb on - strand at 3702.031 kb on + strand at 3702.031 kb on + strand at 3702.032 kb on - strand at 3702.055 kb on + strand at 3702.055 kb on + strand at 3702.055 kb on + strand at 3702.055 kb on + strand at 3702.055 kb on + strand at 3702.056 kb on - strand at 3702.056 kb on - strand at 3702.056 kb on - strand at 3702.056 kb on - strand at 3702.056 kb on - strand at 3702.169 kb on + strand, within Pf1N1B4_3313 at 3702.199 kb on + strand, within Pf1N1B4_3313 at 3702.199 kb on + strand, within Pf1N1B4_3313 at 3702.200 kb on - strand, within Pf1N1B4_3313 at 3702.232 kb on + strand, within Pf1N1B4_3313 at 3702.425 kb on - strand, within Pf1N1B4_3313 at 3702.425 kb on - strand, within Pf1N1B4_3313 at 3702.425 kb on - strand, within Pf1N1B4_3313 at 3702.425 kb on - strand, within Pf1N1B4_3313 at 3702.572 kb on - strand, within Pf1N1B4_3313 at 3702.572 kb on - strand, within Pf1N1B4_3313 at 3702.604 kb on + strand, within Pf1N1B4_3313 at 3702.605 kb on - strand, within Pf1N1B4_3313 at 3702.605 kb on - strand, within Pf1N1B4_3313 at 3702.749 kb on - strand, within Pf1N1B4_3313 at 3702.778 kb on + strand, within Pf1N1B4_3313 at 3702.778 kb on + strand, within Pf1N1B4_3313 at 3702.778 kb on + strand, within Pf1N1B4_3313 at 3702.778 kb on + strand at 3702.778 kb on + strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313 at 3702.779 kb on - strand, within Pf1N1B4_3313
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,700,617 + Pf1N1B4_3311 0.43 +0.6 3,700,618 - Pf1N1B4_3311 0.43 -0.9 3,700,618 - Pf1N1B4_3311 0.43 -0.6 3,700,618 - Pf1N1B4_3311 0.43 -1.4 3,700,634 + -0.1 3,700,634 + Pf1N1B4_3311 0.44 -1.2 3,700,635 - Pf1N1B4_3311 0.44 -1.2 3,700,696 + Pf1N1B4_3311 0.49 -1.1 3,700,881 + Pf1N1B4_3311 0.61 -1.1 3,700,882 - Pf1N1B4_3311 0.61 -1.4 3,700,898 + Pf1N1B4_3311 0.62 -0.1 3,700,898 + Pf1N1B4_3311 0.62 -1.2 3,700,899 - Pf1N1B4_3311 0.62 -0.9 3,701,031 - Pf1N1B4_3311 0.71 -0.5 3,701,031 - Pf1N1B4_3311 0.71 +0.4 3,701,198 - Pf1N1B4_3311 0.82 +1.5 3,701,253 - Pf1N1B4_3311 0.85 +1.1 3,701,268 - Pf1N1B4_3311 0.86 -1.4 3,701,268 - Pf1N1B4_3311 0.86 -1.2 3,701,459 + +0.7 3,701,460 - -0.2 3,701,469 + -1.1 3,701,469 + -0.1 3,701,470 - -0.3 3,701,475 + -0.9 3,701,545 - Pf1N1B4_3312 0.15 -0.1 3,701,578 + Pf1N1B4_3312 0.22 -1.6 3,701,602 - Pf1N1B4_3312 0.27 -1.1 3,701,778 + Pf1N1B4_3312 0.66 -0.4 3,701,779 - Pf1N1B4_3312 0.66 -1.2 3,701,779 - Pf1N1B4_3312 0.66 -1.9 3,701,779 - Pf1N1B4_3312 0.66 +1.2 3,701,779 - +2.5 3,701,779 - Pf1N1B4_3312 0.66 -0.6 3,701,779 - Pf1N1B4_3312 0.66 -1.1 3,701,810 + Pf1N1B4_3312 0.73 -1.4 3,701,927 + -1.9 3,701,927 + -0.6 3,701,927 + +0.2 3,701,927 + +0.9 3,701,927 + -0.1 3,701,927 + -1.3 3,701,927 + +0.6 3,701,927 + -1.9 3,701,927 + +0.9 3,701,927 + -1.1 3,701,928 - +0.2 3,701,928 - +1.4 3,701,928 - -0.5 3,701,928 - -0.8 3,701,928 - +0.0 3,701,928 - -0.6 3,701,928 - -0.1 3,702,031 + -0.3 3,702,031 + +0.9 3,702,032 - -1.4 3,702,055 + +0.5 3,702,055 + -0.4 3,702,055 + -0.1 3,702,055 + +0.9 3,702,055 + +0.1 3,702,056 - -1.1 3,702,056 - -0.2 3,702,056 - +0.5 3,702,056 - -0.6 3,702,056 - +1.6 3,702,169 + Pf1N1B4_3313 0.18 +0.3 3,702,199 + Pf1N1B4_3313 0.21 +1.2 3,702,199 + Pf1N1B4_3313 0.21 -1.8 3,702,200 - Pf1N1B4_3313 0.21 -1.6 3,702,232 + Pf1N1B4_3313 0.24 +0.4 3,702,425 - Pf1N1B4_3313 0.44 -1.1 3,702,425 - Pf1N1B4_3313 0.44 -0.1 3,702,425 - Pf1N1B4_3313 0.44 -0.6 3,702,425 - Pf1N1B4_3313 0.44 -1.9 3,702,572 - Pf1N1B4_3313 0.59 +1.4 3,702,572 - Pf1N1B4_3313 0.59 -1.3 3,702,604 + Pf1N1B4_3313 0.62 +0.1 3,702,605 - Pf1N1B4_3313 0.62 +0.4 3,702,605 - Pf1N1B4_3313 0.62 +1.2 3,702,749 - Pf1N1B4_3313 0.77 +0.5 3,702,778 + Pf1N1B4_3313 0.80 -1.4 3,702,778 + Pf1N1B4_3313 0.80 +1.4 3,702,778 + Pf1N1B4_3313 0.80 -0.4 3,702,778 + -0.6 3,702,778 + Pf1N1B4_3313 0.80 -2.9 3,702,779 - Pf1N1B4_3313 0.80 +1.7 3,702,779 - Pf1N1B4_3313 0.80 -0.1 3,702,779 - Pf1N1B4_3313 0.80 -0.2 3,702,779 - Pf1N1B4_3313 0.80 -0.4 3,702,779 - Pf1N1B4_3313 0.80 +0.0 3,702,779 - Pf1N1B4_3313 0.80 -1.6
Or see this region's nucleotide sequence