Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3271 and Pf1N1B4_3272 are separated by 19 nucleotides Pf1N1B4_3272 and Pf1N1B4_3273 are separated by 79 nucleotides Pf1N1B4_3273 and Pf1N1B4_3274 are separated by 307 nucleotides Pf1N1B4_3274 and Pf1N1B4_3275 are separated by 64 nucleotides Pf1N1B4_3275 and Pf1N1B4_3276 are separated by 30 nucleotides Pf1N1B4_3276 and Pf1N1B4_3277 overlap by 135 nucleotides
Pf1N1B4_3271 - Bicyclomycin resistance protein, at 3,661,251 to 3,662,417
_3271
Pf1N1B4_3272 - hypothetical protein, at 3,662,437 to 3,662,553
_3272
Pf1N1B4_3273 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 3,662,633 to 3,662,893
_3273
Pf1N1B4_3274 - Fosfomycin resistance protein FosA, at 3,663,201 to 3,663,617
_3274
Pf1N1B4_3275 - L-lysine permease, at 3,663,682 to 3,664,320
_3275
Pf1N1B4_3276 - hypothetical protein, at 3,664,351 to 3,664,500
_3276
Pf1N1B4_3277 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 3,664,366 to 3,665,772
_3277
Position (kb)
3663
3664 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3662.272 kb on + strand, within Pf1N1B4_3271 at 3662.272 kb on + strand, within Pf1N1B4_3271 at 3662.272 kb on + strand, within Pf1N1B4_3271 at 3662.273 kb on - strand, within Pf1N1B4_3271 at 3662.273 kb on - strand, within Pf1N1B4_3271 at 3662.273 kb on - strand, within Pf1N1B4_3271 at 3662.314 kb on - strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.347 kb on + strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.348 kb on - strand at 3662.460 kb on - strand, within Pf1N1B4_3272 at 3662.499 kb on + strand, within Pf1N1B4_3272 at 3662.707 kb on + strand, within Pf1N1B4_3273 at 3662.707 kb on + strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3662.708 kb on - strand, within Pf1N1B4_3273 at 3663.139 kb on + strand at 3663.139 kb on + strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.140 kb on - strand at 3663.156 kb on - strand at 3663.156 kb on - strand at 3663.162 kb on + strand at 3663.162 kb on + strand at 3663.163 kb on - strand at 3663.163 kb on - strand at 3663.163 kb on - strand at 3663.184 kb on + strand at 3663.184 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.186 kb on + strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.187 kb on - strand at 3663.191 kb on + strand at 3663.289 kb on + strand, within Pf1N1B4_3274 at 3663.289 kb on + strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.290 kb on - strand, within Pf1N1B4_3274 at 3663.315 kb on + strand, within Pf1N1B4_3274 at 3663.316 kb on - strand, within Pf1N1B4_3274 at 3663.387 kb on + strand, within Pf1N1B4_3274 at 3663.387 kb on + strand, within Pf1N1B4_3274 at 3663.388 kb on - strand, within Pf1N1B4_3274 at 3663.502 kb on - strand, within Pf1N1B4_3274 at 3663.502 kb on - strand at 3663.586 kb on - strand at 3663.640 kb on + strand at 3663.640 kb on + strand at 3663.640 kb on + strand at 3663.641 kb on - strand at 3663.641 kb on - strand at 3663.727 kb on + strand at 3663.727 kb on + strand at 3663.727 kb on + strand at 3663.728 kb on - strand at 3663.728 kb on - strand at 3663.866 kb on + strand, within Pf1N1B4_3275 at 3663.925 kb on + strand, within Pf1N1B4_3275 at 3663.925 kb on + strand, within Pf1N1B4_3275 at 3663.926 kb on - strand, within Pf1N1B4_3275 at 3664.072 kb on - strand, within Pf1N1B4_3275 at 3664.072 kb on - strand, within Pf1N1B4_3275 at 3664.162 kb on + strand, within Pf1N1B4_3275 at 3664.167 kb on + strand, within Pf1N1B4_3275 at 3664.175 kb on + strand, within Pf1N1B4_3275 at 3664.176 kb on - strand, within Pf1N1B4_3275 at 3664.210 kb on + strand, within Pf1N1B4_3275 at 3664.211 kb on - strand, within Pf1N1B4_3275 at 3664.211 kb on - strand, within Pf1N1B4_3275 at 3664.211 kb on - strand, within Pf1N1B4_3275 at 3664.211 kb on - strand at 3664.251 kb on - strand, within Pf1N1B4_3275 at 3664.346 kb on + strand at 3664.346 kb on + strand at 3664.347 kb on - strand at 3664.347 kb on - strand at 3664.347 kb on - strand at 3664.347 kb on - strand at 3664.365 kb on + strand at 3664.366 kb on - strand at 3664.456 kb on + strand at 3664.456 kb on + strand at 3664.456 kb on + strand at 3664.456 kb on + strand at 3664.457 kb on - strand at 3664.457 kb on - strand at 3664.495 kb on - strand at 3664.511 kb on + strand, within Pf1N1B4_3277 at 3664.512 kb on - strand at 3664.589 kb on + strand, within Pf1N1B4_3277 at 3664.589 kb on + strand, within Pf1N1B4_3277 at 3664.590 kb on - strand, within Pf1N1B4_3277 at 3664.590 kb on - strand, within Pf1N1B4_3277
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,662,272 + Pf1N1B4_3271 0.87 +0.7 3,662,272 + Pf1N1B4_3271 0.87 -0.9 3,662,272 + Pf1N1B4_3271 0.87 -0.1 3,662,273 - Pf1N1B4_3271 0.88 -0.6 3,662,273 - Pf1N1B4_3271 0.88 +0.4 3,662,273 - Pf1N1B4_3271 0.88 -2.4 3,662,314 - -0.4 3,662,347 + -1.1 3,662,347 + -0.1 3,662,347 + +1.1 3,662,347 + +0.0 3,662,347 + -0.1 3,662,347 + -0.1 3,662,348 - -0.4 3,662,348 - +0.9 3,662,348 - -0.6 3,662,348 - +1.9 3,662,348 - +1.1 3,662,348 - +0.8 3,662,348 - -0.6 3,662,348 - -3.4 3,662,348 - -0.6 3,662,460 - Pf1N1B4_3272 0.20 +1.1 3,662,499 + Pf1N1B4_3272 0.53 -0.7 3,662,707 + Pf1N1B4_3273 0.28 -2.1 3,662,707 + Pf1N1B4_3273 0.28 +0.9 3,662,708 - Pf1N1B4_3273 0.29 +0.9 3,662,708 - Pf1N1B4_3273 0.29 -1.1 3,662,708 - Pf1N1B4_3273 0.29 -0.6 3,663,139 + +1.6 3,663,139 + -0.2 3,663,140 - -1.8 3,663,140 - +0.3 3,663,140 - +0.9 3,663,140 - +0.6 3,663,140 - -0.5 3,663,156 - +0.9 3,663,156 - -0.9 3,663,162 + +0.9 3,663,162 + -0.8 3,663,163 - -0.7 3,663,163 - +0.2 3,663,163 - +0.9 3,663,184 + -1.1 3,663,184 + +2.3 3,663,186 + -1.4 3,663,186 + -0.4 3,663,186 + +0.9 3,663,186 + -0.1 3,663,186 + -1.4 3,663,187 - +0.2 3,663,187 - +0.3 3,663,187 - +1.5 3,663,187 - +0.6 3,663,187 - -1.1 3,663,187 - +0.9 3,663,191 + +0.9 3,663,289 + Pf1N1B4_3274 0.21 -0.3 3,663,289 + Pf1N1B4_3274 0.21 +0.4 3,663,290 - Pf1N1B4_3274 0.21 -0.7 3,663,290 - Pf1N1B4_3274 0.21 -1.4 3,663,290 - Pf1N1B4_3274 0.21 -0.8 3,663,315 + Pf1N1B4_3274 0.27 +0.5 3,663,316 - Pf1N1B4_3274 0.28 -3.0 3,663,387 + Pf1N1B4_3274 0.45 -1.1 3,663,387 + Pf1N1B4_3274 0.45 -1.1 3,663,388 - Pf1N1B4_3274 0.45 -1.1 3,663,502 - Pf1N1B4_3274 0.72 -1.1 3,663,502 - -0.7 3,663,586 - -0.4 3,663,640 + +0.5 3,663,640 + -0.7 3,663,640 + +0.2 3,663,641 - +1.7 3,663,641 - -0.7 3,663,727 + -0.2 3,663,727 + +0.9 3,663,727 + +1.1 3,663,728 - -0.1 3,663,728 - +0.1 3,663,866 + Pf1N1B4_3275 0.29 -1.4 3,663,925 + Pf1N1B4_3275 0.38 -1.1 3,663,925 + Pf1N1B4_3275 0.38 -0.4 3,663,926 - Pf1N1B4_3275 0.38 -0.1 3,664,072 - Pf1N1B4_3275 0.61 +1.7 3,664,072 - Pf1N1B4_3275 0.61 -0.1 3,664,162 + Pf1N1B4_3275 0.75 +0.3 3,664,167 + Pf1N1B4_3275 0.76 +0.6 3,664,175 + Pf1N1B4_3275 0.77 -0.2 3,664,176 - Pf1N1B4_3275 0.77 -0.1 3,664,210 + Pf1N1B4_3275 0.83 +0.1 3,664,211 - Pf1N1B4_3275 0.83 +0.7 3,664,211 - Pf1N1B4_3275 0.83 +1.7 3,664,211 - Pf1N1B4_3275 0.83 -1.2 3,664,211 - -1.1 3,664,251 - Pf1N1B4_3275 0.89 +0.9 3,664,346 + -1.1 3,664,346 + -1.9 3,664,347 - -1.1 3,664,347 - -0.7 3,664,347 - +0.8 3,664,347 - +0.3 3,664,365 + +0.7 3,664,366 - -0.9 3,664,456 + -2.4 3,664,456 + +0.3 3,664,456 + +0.6 3,664,456 + -0.3 3,664,457 - +0.4 3,664,457 - -0.3 3,664,495 - -1.1 3,664,511 + Pf1N1B4_3277 0.10 +1.5 3,664,512 - +0.9 3,664,589 + Pf1N1B4_3277 0.16 -0.1 3,664,589 + Pf1N1B4_3277 0.16 +1.7 3,664,590 - Pf1N1B4_3277 0.16 -1.4 3,664,590 - Pf1N1B4_3277 0.16 +0.5
Or see this region's nucleotide sequence