Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3131 and Pf1N1B4_3132 overlap by 13 nucleotides Pf1N1B4_3132 and Pf1N1B4_3133 are separated by 11 nucleotides
Pf1N1B4_3131 - Phage FluMu protein gp47, at 3,515,997 to 3,517,040
_3131
Pf1N1B4_3132 - FIG121501: Prophage tail protein, at 3,517,028 to 3,517,627
_3132
Pf1N1B4_3133 - Prophage tail fiber protein, at 3,517,639 to 3,518,787
_3133
Position (kb)
3517
3518 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3516.324 kb on + strand, within Pf1N1B4_3131 at 3516.324 kb on + strand, within Pf1N1B4_3131 at 3516.324 kb on + strand, within Pf1N1B4_3131 at 3516.325 kb on - strand, within Pf1N1B4_3131 at 3516.325 kb on - strand, within Pf1N1B4_3131 at 3516.465 kb on - strand, within Pf1N1B4_3131 at 3516.465 kb on - strand, within Pf1N1B4_3131 at 3516.585 kb on + strand, within Pf1N1B4_3131 at 3516.585 kb on + strand, within Pf1N1B4_3131 at 3516.586 kb on - strand, within Pf1N1B4_3131 at 3516.617 kb on - strand, within Pf1N1B4_3131 at 3516.669 kb on + strand, within Pf1N1B4_3131 at 3516.669 kb on + strand at 3516.669 kb on + strand, within Pf1N1B4_3131 at 3516.669 kb on + strand, within Pf1N1B4_3131 at 3516.669 kb on + strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.670 kb on - strand, within Pf1N1B4_3131 at 3516.735 kb on + strand at 3516.735 kb on + strand, within Pf1N1B4_3131 at 3516.735 kb on + strand, within Pf1N1B4_3131 at 3516.736 kb on - strand, within Pf1N1B4_3131 at 3516.736 kb on - strand, within Pf1N1B4_3131 at 3516.736 kb on - strand, within Pf1N1B4_3131 at 3516.736 kb on - strand, within Pf1N1B4_3131 at 3516.769 kb on + strand, within Pf1N1B4_3131 at 3516.769 kb on + strand, within Pf1N1B4_3131 at 3516.769 kb on + strand, within Pf1N1B4_3131 at 3516.769 kb on + strand, within Pf1N1B4_3131 at 3516.769 kb on + strand at 3516.770 kb on - strand, within Pf1N1B4_3131 at 3516.770 kb on - strand, within Pf1N1B4_3131 at 3516.770 kb on - strand, within Pf1N1B4_3131 at 3516.770 kb on - strand, within Pf1N1B4_3131 at 3516.804 kb on + strand, within Pf1N1B4_3131 at 3516.805 kb on - strand, within Pf1N1B4_3131 at 3516.805 kb on - strand, within Pf1N1B4_3131 at 3516.838 kb on + strand, within Pf1N1B4_3131 at 3517.028 kb on - strand at 3517.028 kb on - strand at 3517.028 kb on - strand at 3517.028 kb on - strand at 3517.028 kb on - strand at 3517.028 kb on - strand at 3517.038 kb on + strand at 3517.038 kb on + strand at 3517.038 kb on + strand at 3517.038 kb on + strand at 3517.039 kb on - strand at 3517.039 kb on - strand at 3517.039 kb on - strand at 3517.349 kb on + strand, within Pf1N1B4_3132 at 3517.349 kb on + strand, within Pf1N1B4_3132 at 3517.349 kb on + strand, within Pf1N1B4_3132 at 3517.350 kb on - strand, within Pf1N1B4_3132 at 3517.350 kb on - strand, within Pf1N1B4_3132 at 3517.526 kb on + strand, within Pf1N1B4_3132 at 3517.527 kb on - strand, within Pf1N1B4_3132 at 3517.619 kb on + strand at 3517.619 kb on + strand at 3517.619 kb on + strand at 3517.619 kb on + strand at 3517.619 kb on + strand at 3517.619 kb on + strand at 3517.620 kb on - strand at 3517.620 kb on - strand at 3517.620 kb on - strand at 3517.620 kb on - strand at 3517.620 kb on - strand at 3517.813 kb on + strand at 3517.813 kb on + strand, within Pf1N1B4_3133 at 3517.813 kb on + strand, within Pf1N1B4_3133 at 3517.813 kb on + strand, within Pf1N1B4_3133 at 3517.814 kb on - strand, within Pf1N1B4_3133 at 3518.090 kb on - strand at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.132 kb on - strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.153 kb on - strand, within Pf1N1B4_3133 at 3518.290 kb on + strand at 3518.291 kb on - strand, within Pf1N1B4_3133 at 3518.291 kb on - strand, within Pf1N1B4_3133 at 3518.344 kb on + strand, within Pf1N1B4_3133 at 3518.344 kb on + strand, within Pf1N1B4_3133 at 3518.345 kb on - strand, within Pf1N1B4_3133 at 3518.345 kb on - strand, within Pf1N1B4_3133 at 3518.461 kb on + strand, within Pf1N1B4_3133 at 3518.461 kb on + strand, within Pf1N1B4_3133 at 3518.541 kb on + strand, within Pf1N1B4_3133 at 3518.548 kb on - strand, within Pf1N1B4_3133 at 3518.627 kb on - strand, within Pf1N1B4_3133
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,516,324 + Pf1N1B4_3131 0.31 -1.3 3,516,324 + Pf1N1B4_3131 0.31 +1.0 3,516,324 + Pf1N1B4_3131 0.31 +0.2 3,516,325 - Pf1N1B4_3131 0.31 +0.3 3,516,325 - Pf1N1B4_3131 0.31 +0.4 3,516,465 - Pf1N1B4_3131 0.45 +1.0 3,516,465 - Pf1N1B4_3131 0.45 +0.1 3,516,585 + Pf1N1B4_3131 0.56 +2.3 3,516,585 + Pf1N1B4_3131 0.56 -0.2 3,516,586 - Pf1N1B4_3131 0.56 -0.2 3,516,617 - Pf1N1B4_3131 0.59 +1.0 3,516,669 + Pf1N1B4_3131 0.64 -0.1 3,516,669 + +0.0 3,516,669 + Pf1N1B4_3131 0.64 +0.0 3,516,669 + Pf1N1B4_3131 0.64 -2.2 3,516,669 + Pf1N1B4_3131 0.64 +0.0 3,516,670 - Pf1N1B4_3131 0.64 +0.0 3,516,670 - Pf1N1B4_3131 0.64 -1.4 3,516,670 - Pf1N1B4_3131 0.64 -1.0 3,516,670 - Pf1N1B4_3131 0.64 -0.3 3,516,670 - Pf1N1B4_3131 0.64 +0.9 3,516,735 + +0.0 3,516,735 + Pf1N1B4_3131 0.71 +0.3 3,516,735 + Pf1N1B4_3131 0.71 -1.0 3,516,736 - Pf1N1B4_3131 0.71 +1.8 3,516,736 - Pf1N1B4_3131 0.71 +1.5 3,516,736 - Pf1N1B4_3131 0.71 +0.3 3,516,736 - Pf1N1B4_3131 0.71 +0.5 3,516,769 + Pf1N1B4_3131 0.74 -0.4 3,516,769 + Pf1N1B4_3131 0.74 -0.8 3,516,769 + Pf1N1B4_3131 0.74 +1.9 3,516,769 + Pf1N1B4_3131 0.74 +0.0 3,516,769 + +1.0 3,516,770 - Pf1N1B4_3131 0.74 +2.2 3,516,770 - Pf1N1B4_3131 0.74 -1.0 3,516,770 - Pf1N1B4_3131 0.74 -0.6 3,516,770 - Pf1N1B4_3131 0.74 +1.8 3,516,804 + Pf1N1B4_3131 0.77 +1.0 3,516,805 - Pf1N1B4_3131 0.77 +1.5 3,516,805 - Pf1N1B4_3131 0.77 -1.1 3,516,838 + Pf1N1B4_3131 0.81 +0.7 3,517,028 - +0.6 3,517,028 - -0.4 3,517,028 - +0.0 3,517,028 - -1.0 3,517,028 - +1.3 3,517,028 - +0.2 3,517,038 + -0.3 3,517,038 + +0.6 3,517,038 + -1.7 3,517,038 + +1.0 3,517,039 - -1.8 3,517,039 - +1.0 3,517,039 - +0.9 3,517,349 + Pf1N1B4_3132 0.54 -1.8 3,517,349 + Pf1N1B4_3132 0.54 -1.8 3,517,349 + Pf1N1B4_3132 0.54 -1.2 3,517,350 - Pf1N1B4_3132 0.54 -0.5 3,517,350 - Pf1N1B4_3132 0.54 -0.6 3,517,526 + Pf1N1B4_3132 0.83 +1.0 3,517,527 - Pf1N1B4_3132 0.83 -1.0 3,517,619 + -0.5 3,517,619 + -0.2 3,517,619 + +2.6 3,517,619 + +1.7 3,517,619 + -1.3 3,517,619 + +0.3 3,517,620 - -0.3 3,517,620 - +2.4 3,517,620 - -0.7 3,517,620 - +2.6 3,517,620 - +1.7 3,517,813 + +2.0 3,517,813 + Pf1N1B4_3133 0.15 +0.0 3,517,813 + Pf1N1B4_3133 0.15 -1.3 3,517,813 + Pf1N1B4_3133 0.15 +1.0 3,517,814 - Pf1N1B4_3133 0.15 -0.3 3,518,090 - +1.0 3,518,131 + Pf1N1B4_3133 0.43 -0.3 3,518,131 + Pf1N1B4_3133 0.43 -0.3 3,518,131 + Pf1N1B4_3133 0.43 +0.0 3,518,131 + Pf1N1B4_3133 0.43 -0.7 3,518,131 + Pf1N1B4_3133 0.43 +0.2 3,518,131 + Pf1N1B4_3133 0.43 -0.6 3,518,131 + Pf1N1B4_3133 0.43 -0.4 3,518,132 - Pf1N1B4_3133 0.43 +1.8 3,518,152 + Pf1N1B4_3133 0.45 -1.6 3,518,152 + Pf1N1B4_3133 0.45 +0.2 3,518,152 + Pf1N1B4_3133 0.45 -0.1 3,518,152 + Pf1N1B4_3133 0.45 +0.1 3,518,152 + Pf1N1B4_3133 0.45 -1.0 3,518,152 + Pf1N1B4_3133 0.45 +0.0 3,518,152 + Pf1N1B4_3133 0.45 +0.4 3,518,153 - Pf1N1B4_3133 0.45 -0.6 3,518,290 + -0.6 3,518,291 - Pf1N1B4_3133 0.57 +0.3 3,518,291 - Pf1N1B4_3133 0.57 +1.6 3,518,344 + Pf1N1B4_3133 0.61 +1.6 3,518,344 + Pf1N1B4_3133 0.61 -2.3 3,518,345 - Pf1N1B4_3133 0.61 +0.2 3,518,345 - Pf1N1B4_3133 0.61 +1.0 3,518,461 + Pf1N1B4_3133 0.72 +0.3 3,518,461 + Pf1N1B4_3133 0.72 +0.4 3,518,541 + Pf1N1B4_3133 0.79 -1.0 3,518,548 - Pf1N1B4_3133 0.79 -0.3 3,518,627 - Pf1N1B4_3133 0.86 -0.2
Or see this region's nucleotide sequence