Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2968 and Pf1N1B4_2969 are separated by 90 nucleotides Pf1N1B4_2969 and Pf1N1B4_2970 are separated by 268 nucleotides
Pf1N1B4_2968 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 3,328,963 to 3,330,318
_2968
Pf1N1B4_2969 - ATPase, AFG1 family, at 3,330,409 to 3,331,503
_2969
Pf1N1B4_2970 - FIG00959654: hypothetical protein, at 3,331,772 to 3,332,476
_2970
Position (kb)
3330
3331
3332 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3330.371 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.409 kb on - strand at 3330.419 kb on + strand at 3330.419 kb on + strand at 3330.419 kb on + strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.420 kb on - strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.505 kb on + strand at 3330.506 kb on - strand at 3330.506 kb on - strand at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.595 kb on + strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.596 kb on - strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.628 kb on + strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.629 kb on - strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.916 kb on + strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3330.917 kb on - strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.136 kb on + strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.137 kb on - strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.240 kb on + strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.241 kb on - strand, within Pf1N1B4_2969 at 3331.329 kb on + strand, within Pf1N1B4_2969 at 3331.329 kb on + strand, within Pf1N1B4_2969 at 3331.329 kb on + strand, within Pf1N1B4_2969 at 3331.329 kb on + strand, within Pf1N1B4_2969 at 3331.330 kb on - strand, within Pf1N1B4_2969 at 3331.351 kb on + strand, within Pf1N1B4_2969 at 3331.351 kb on + strand, within Pf1N1B4_2969 at 3331.351 kb on + strand, within Pf1N1B4_2969 at 3331.351 kb on + strand, within Pf1N1B4_2969 at 3331.352 kb on - strand, within Pf1N1B4_2969 at 3331.405 kb on + strand at 3331.405 kb on + strand at 3331.405 kb on + strand at 3331.405 kb on + strand at 3331.406 kb on - strand at 3331.406 kb on - strand at 3331.406 kb on - strand at 3331.406 kb on - strand at 3331.406 kb on - strand at 3331.406 kb on - strand at 3331.501 kb on + strand at 3331.501 kb on + strand at 3331.501 kb on + strand at 3331.501 kb on + strand at 3331.502 kb on - strand at 3331.502 kb on - strand at 3331.515 kb on + strand at 3331.515 kb on + strand at 3331.553 kb on - strand at 3331.568 kb on + strand at 3331.569 kb on - strand at 3331.569 kb on - strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.650 kb on - strand at 3331.650 kb on - strand at 3331.691 kb on + strand at 3331.691 kb on + strand at 3331.692 kb on - strand at 3331.709 kb on - strand at 3331.723 kb on + strand at 3331.724 kb on - strand at 3331.765 kb on + strand at 3331.765 kb on + strand at 3331.766 kb on - strand at 3331.766 kb on - strand at 3331.766 kb on - strand at 3331.766 kb on - strand at 3331.766 kb on - strand at 3331.767 kb on + strand at 3331.767 kb on + strand at 3331.768 kb on - strand at 3331.768 kb on - strand at 3331.774 kb on - strand at 3331.792 kb on - strand at 3332.102 kb on + strand, within Pf1N1B4_2970 at 3332.489 kb on - strand at 3332.497 kb on + strand at 3332.498 kb on - strand at 3332.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,330,371 - -0.7 3,330,409 - +1.1 3,330,409 - -0.5 3,330,409 - +0.3 3,330,409 - -0.4 3,330,409 - +0.8 3,330,409 - -0.9 3,330,409 - +1.7 3,330,419 + +0.9 3,330,419 + +0.7 3,330,419 + +0.7 3,330,420 - +0.5 3,330,420 - +0.7 3,330,420 - +2.3 3,330,420 - -0.1 3,330,420 - +0.2 3,330,420 - -0.2 3,330,505 + +0.1 3,330,505 + -0.1 3,330,505 + +1.7 3,330,505 + +0.7 3,330,505 + -0.1 3,330,506 - -0.3 3,330,506 - +1.9 3,330,595 + Pf1N1B4_2969 0.17 +1.0 3,330,595 + Pf1N1B4_2969 0.17 +0.3 3,330,595 + Pf1N1B4_2969 0.17 +0.7 3,330,595 + Pf1N1B4_2969 0.17 +1.1 3,330,595 + Pf1N1B4_2969 0.17 +0.6 3,330,595 + Pf1N1B4_2969 0.17 -0.0 3,330,595 + Pf1N1B4_2969 0.17 +1.8 3,330,595 + Pf1N1B4_2969 0.17 +2.7 3,330,596 - Pf1N1B4_2969 0.17 +1.3 3,330,596 - Pf1N1B4_2969 0.17 +1.5 3,330,596 - Pf1N1B4_2969 0.17 +0.8 3,330,596 - Pf1N1B4_2969 0.17 +1.7 3,330,596 - Pf1N1B4_2969 0.17 +0.8 3,330,596 - Pf1N1B4_2969 0.17 +0.4 3,330,596 - Pf1N1B4_2969 0.17 +1.7 3,330,596 - Pf1N1B4_2969 0.17 -0.1 3,330,596 - Pf1N1B4_2969 0.17 +0.5 3,330,596 - Pf1N1B4_2969 0.17 +1.2 3,330,596 - Pf1N1B4_2969 0.17 +1.5 3,330,596 - Pf1N1B4_2969 0.17 +0.8 3,330,628 + Pf1N1B4_2969 0.20 +0.5 3,330,628 + Pf1N1B4_2969 0.20 +1.6 3,330,628 + Pf1N1B4_2969 0.20 -0.5 3,330,628 + Pf1N1B4_2969 0.20 +1.1 3,330,628 + Pf1N1B4_2969 0.20 +1.1 3,330,628 + Pf1N1B4_2969 0.20 -0.0 3,330,628 + Pf1N1B4_2969 0.20 +1.3 3,330,628 + Pf1N1B4_2969 0.20 +0.3 3,330,628 + Pf1N1B4_2969 0.20 -0.5 3,330,628 + Pf1N1B4_2969 0.20 -0.9 3,330,628 + Pf1N1B4_2969 0.20 -0.5 3,330,628 + Pf1N1B4_2969 0.20 +0.7 3,330,628 + Pf1N1B4_2969 0.20 -0.7 3,330,628 + Pf1N1B4_2969 0.20 +2.2 3,330,629 - Pf1N1B4_2969 0.20 +0.1 3,330,629 - Pf1N1B4_2969 0.20 +1.4 3,330,629 - Pf1N1B4_2969 0.20 +0.5 3,330,629 - Pf1N1B4_2969 0.20 +0.4 3,330,629 - Pf1N1B4_2969 0.20 +0.1 3,330,629 - Pf1N1B4_2969 0.20 +0.8 3,330,629 - Pf1N1B4_2969 0.20 +0.1 3,330,629 - Pf1N1B4_2969 0.20 -0.0 3,330,629 - Pf1N1B4_2969 0.20 +1.7 3,330,629 - Pf1N1B4_2969 0.20 +1.0 3,330,629 - Pf1N1B4_2969 0.20 +2.2 3,330,629 - Pf1N1B4_2969 0.20 +0.7 3,330,629 - Pf1N1B4_2969 0.20 +1.4 3,330,629 - Pf1N1B4_2969 0.20 +1.0 3,330,629 - Pf1N1B4_2969 0.20 +1.1 3,330,629 - Pf1N1B4_2969 0.20 +0.7 3,330,629 - Pf1N1B4_2969 0.20 +1.5 3,330,916 + Pf1N1B4_2969 0.46 -0.6 3,330,916 + Pf1N1B4_2969 0.46 +1.1 3,330,916 + Pf1N1B4_2969 0.46 +0.9 3,330,916 + Pf1N1B4_2969 0.46 +1.5 3,330,916 + Pf1N1B4_2969 0.46 -0.6 3,330,916 + Pf1N1B4_2969 0.46 +0.3 3,330,916 + +2.9 3,330,916 + Pf1N1B4_2969 0.46 -1.3 3,330,916 + Pf1N1B4_2969 0.46 +0.4 3,330,916 + Pf1N1B4_2969 0.46 -0.3 3,330,916 + Pf1N1B4_2969 0.46 +0.6 3,330,917 - Pf1N1B4_2969 0.46 +0.3 3,330,917 - Pf1N1B4_2969 0.46 -0.3 3,330,917 - Pf1N1B4_2969 0.46 -1.3 3,330,917 - Pf1N1B4_2969 0.46 -0.0 3,330,917 - Pf1N1B4_2969 0.46 +1.3 3,330,917 - Pf1N1B4_2969 0.46 +1.1 3,330,917 - Pf1N1B4_2969 0.46 -0.7 3,331,136 + Pf1N1B4_2969 0.66 -0.1 3,331,136 + Pf1N1B4_2969 0.66 +0.9 3,331,136 + Pf1N1B4_2969 0.66 +0.1 3,331,136 + Pf1N1B4_2969 0.66 +0.8 3,331,136 + Pf1N1B4_2969 0.66 -0.3 3,331,136 + Pf1N1B4_2969 0.66 +1.4 3,331,136 + Pf1N1B4_2969 0.66 +0.5 3,331,136 + Pf1N1B4_2969 0.66 -0.7 3,331,136 + Pf1N1B4_2969 0.66 +0.4 3,331,136 + Pf1N1B4_2969 0.66 -0.0 3,331,136 + Pf1N1B4_2969 0.66 +1.7 3,331,136 + Pf1N1B4_2969 0.66 +1.4 3,331,136 + Pf1N1B4_2969 0.66 +1.2 3,331,136 + Pf1N1B4_2969 0.66 +0.5 3,331,136 + Pf1N1B4_2969 0.66 +0.6 3,331,136 + Pf1N1B4_2969 0.66 -0.9 3,331,136 + Pf1N1B4_2969 0.66 -2.3 3,331,136 + Pf1N1B4_2969 0.66 +0.6 3,331,136 + Pf1N1B4_2969 0.66 +0.1 3,331,136 + Pf1N1B4_2969 0.66 -0.4 3,331,136 + +1.1 3,331,136 + Pf1N1B4_2969 0.66 -0.2 3,331,136 + Pf1N1B4_2969 0.66 +2.2 3,331,137 - Pf1N1B4_2969 0.66 +0.1 3,331,137 - Pf1N1B4_2969 0.66 +1.1 3,331,137 - Pf1N1B4_2969 0.66 +0.9 3,331,137 - Pf1N1B4_2969 0.66 +0.4 3,331,137 - Pf1N1B4_2969 0.66 +0.8 3,331,137 - Pf1N1B4_2969 0.66 +0.9 3,331,137 - Pf1N1B4_2969 0.66 +0.1 3,331,137 - Pf1N1B4_2969 0.66 +0.1 3,331,137 - Pf1N1B4_2969 0.66 +1.1 3,331,137 - Pf1N1B4_2969 0.66 +0.5 3,331,137 - Pf1N1B4_2969 0.66 +1.2 3,331,137 - Pf1N1B4_2969 0.66 +0.7 3,331,240 + Pf1N1B4_2969 0.76 +0.8 3,331,240 + Pf1N1B4_2969 0.76 +0.7 3,331,240 + +0.5 3,331,240 + Pf1N1B4_2969 0.76 +0.2 3,331,240 + Pf1N1B4_2969 0.76 -1.3 3,331,240 + Pf1N1B4_2969 0.76 +0.9 3,331,240 + Pf1N1B4_2969 0.76 +1.8 3,331,240 + Pf1N1B4_2969 0.76 +2.1 3,331,240 + Pf1N1B4_2969 0.76 +1.2 3,331,240 + Pf1N1B4_2969 0.76 +1.4 3,331,241 - Pf1N1B4_2969 0.76 +0.1 3,331,241 - Pf1N1B4_2969 0.76 -0.3 3,331,241 - Pf1N1B4_2969 0.76 +2.1 3,331,241 - Pf1N1B4_2969 0.76 -0.2 3,331,241 - Pf1N1B4_2969 0.76 -0.5 3,331,241 - Pf1N1B4_2969 0.76 -0.5 3,331,241 - Pf1N1B4_2969 0.76 +1.1 3,331,329 + Pf1N1B4_2969 0.84 +1.4 3,331,329 + Pf1N1B4_2969 0.84 +2.3 3,331,329 + Pf1N1B4_2969 0.84 +0.6 3,331,329 + Pf1N1B4_2969 0.84 +0.2 3,331,330 - Pf1N1B4_2969 0.84 +0.8 3,331,351 + Pf1N1B4_2969 0.86 +0.4 3,331,351 + Pf1N1B4_2969 0.86 +1.1 3,331,351 + Pf1N1B4_2969 0.86 +0.3 3,331,351 + Pf1N1B4_2969 0.86 +1.3 3,331,352 - Pf1N1B4_2969 0.86 +1.1 3,331,405 + +0.2 3,331,405 + -1.3 3,331,405 + -0.3 3,331,405 + -0.2 3,331,406 - +1.8 3,331,406 - +0.4 3,331,406 - +1.7 3,331,406 - +1.1 3,331,406 - +0.2 3,331,406 - +0.3 3,331,501 + +0.1 3,331,501 + -0.7 3,331,501 + -1.3 3,331,501 + +0.2 3,331,502 - +3.4 3,331,502 - -1.4 3,331,515 + -0.3 3,331,515 + +2.2 3,331,553 - -0.3 3,331,568 + -0.9 3,331,569 - -0.7 3,331,569 - -0.9 3,331,649 + +0.1 3,331,649 + +0.1 3,331,649 + -0.2 3,331,649 + +0.4 3,331,649 + -1.8 3,331,649 + -0.5 3,331,649 + +1.1 3,331,649 + +1.1 3,331,650 - +0.7 3,331,650 - -1.6 3,331,691 + +0.1 3,331,691 + +0.7 3,331,692 - +0.7 3,331,709 - +1.7 3,331,723 + +1.1 3,331,724 - -0.7 3,331,765 + +0.8 3,331,765 + -0.9 3,331,766 - -2.3 3,331,766 - +0.1 3,331,766 - -3.0 3,331,766 - -2.6 3,331,766 - -1.6 3,331,767 + +0.7 3,331,767 + -2.1 3,331,768 - +1.1 3,331,768 - +0.7 3,331,774 - -1.5 3,331,792 - +1.1 3,332,102 + Pf1N1B4_2970 0.47 +0.7 3,332,489 - +1.1 3,332,497 + -1.5 3,332,498 - -1.1 3,332,498 - +2.7
Or see this region's nucleotide sequence