Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2954 and Pf1N1B4_2955 are separated by 271 nucleotides Pf1N1B4_2955 and Pf1N1B4_2956 are separated by 7 nucleotides Pf1N1B4_2956 and Pf1N1B4_2957 are separated by 142 nucleotides
Pf1N1B4_2954 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,308,384 to 3,309,349
_2954
Pf1N1B4_2955 - L-lysine permease, at 3,309,621 to 3,310,250
_2955
Pf1N1B4_2956 - hypothetical protein, at 3,310,258 to 3,310,386
_2956
Pf1N1B4_2957 - Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-), at 3,310,529 to 3,311,830
_2957
Position (kb)
3309
3310
3311 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3309.006 kb on - strand, within Pf1N1B4_2954 at 3309.356 kb on + strand at 3309.357 kb on - strand at 3309.397 kb on + strand at 3309.397 kb on + strand at 3309.398 kb on - strand at 3309.398 kb on - strand at 3309.443 kb on + strand at 3309.443 kb on + strand at 3309.443 kb on + strand at 3309.444 kb on - strand at 3309.481 kb on + strand at 3309.481 kb on + strand at 3309.481 kb on + strand at 3309.482 kb on - strand at 3309.527 kb on + strand at 3309.528 kb on - strand at 3309.539 kb on + strand at 3309.539 kb on + strand at 3309.540 kb on - strand at 3309.624 kb on + strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.625 kb on - strand at 3309.864 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.873 kb on + strand, within Pf1N1B4_2955 at 3309.874 kb on - strand, within Pf1N1B4_2955 at 3309.874 kb on - strand, within Pf1N1B4_2955 at 3310.086 kb on + strand, within Pf1N1B4_2955 at 3310.086 kb on + strand, within Pf1N1B4_2955 at 3310.087 kb on - strand, within Pf1N1B4_2955 at 3310.087 kb on - strand, within Pf1N1B4_2955 at 3310.087 kb on - strand, within Pf1N1B4_2955 at 3310.087 kb on - strand, within Pf1N1B4_2955 at 3310.259 kb on + strand at 3310.259 kb on + strand at 3310.259 kb on + strand at 3310.259 kb on + strand at 3310.259 kb on + strand at 3310.260 kb on - strand at 3310.382 kb on + strand at 3310.382 kb on + strand at 3310.382 kb on + strand at 3310.382 kb on + strand at 3310.382 kb on + strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.383 kb on - strand at 3310.478 kb on - strand at 3310.478 kb on - strand at 3310.478 kb on - strand at 3310.478 kb on - strand at 3310.486 kb on + strand at 3310.486 kb on + strand at 3310.486 kb on + strand at 3310.486 kb on + strand at 3310.486 kb on + strand at 3310.487 kb on - strand at 3310.487 kb on - strand at 3310.487 kb on - strand at 3310.487 kb on - strand at 3310.492 kb on + strand at 3310.493 kb on - strand at 3310.528 kb on + strand at 3310.528 kb on + strand at 3310.528 kb on + strand at 3310.528 kb on + strand at 3310.528 kb on + strand at 3310.529 kb on - strand at 3310.529 kb on - strand at 3310.529 kb on - strand at 3310.529 kb on - strand at 3310.529 kb on - strand at 3310.569 kb on - strand at 3310.640 kb on + strand at 3310.853 kb on + strand, within Pf1N1B4_2957 at 3310.853 kb on + strand, within Pf1N1B4_2957 at 3310.853 kb on + strand, within Pf1N1B4_2957 at 3310.913 kb on + strand, within Pf1N1B4_2957 at 3310.913 kb on + strand, within Pf1N1B4_2957 at 3310.913 kb on + strand, within Pf1N1B4_2957 at 3310.913 kb on + strand, within Pf1N1B4_2957 at 3310.914 kb on - strand, within Pf1N1B4_2957 at 3310.914 kb on - strand at 3311.012 kb on + strand, within Pf1N1B4_2957 at 3311.013 kb on - strand, within Pf1N1B4_2957 at 3311.013 kb on - strand, within Pf1N1B4_2957 at 3311.045 kb on + strand, within Pf1N1B4_2957 at 3311.046 kb on - strand, within Pf1N1B4_2957 at 3311.090 kb on + strand, within Pf1N1B4_2957 at 3311.090 kb on + strand, within Pf1N1B4_2957 at 3311.090 kb on + strand, within Pf1N1B4_2957 at 3311.091 kb on - strand, within Pf1N1B4_2957 at 3311.126 kb on + strand, within Pf1N1B4_2957 at 3311.126 kb on + strand, within Pf1N1B4_2957 at 3311.126 kb on + strand, within Pf1N1B4_2957 at 3311.127 kb on - strand, within Pf1N1B4_2957 at 3311.127 kb on - strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.222 kb on + strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957 at 3311.223 kb on - strand, within Pf1N1B4_2957
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,309,006 - Pf1N1B4_2954 0.64 -0.2 3,309,356 + -0.7 3,309,357 - +0.3 3,309,397 + +0.9 3,309,397 + +0.1 3,309,398 - +0.3 3,309,398 - -1.9 3,309,443 + +0.5 3,309,443 + +1.4 3,309,443 + +0.6 3,309,444 - -0.2 3,309,481 + +0.8 3,309,481 + -0.4 3,309,481 + +0.9 3,309,482 - +0.1 3,309,527 + +0.1 3,309,528 - -0.8 3,309,539 + -0.9 3,309,539 + -0.3 3,309,540 - +0.5 3,309,624 + +1.5 3,309,625 - -0.9 3,309,625 - +0.5 3,309,625 - +1.5 3,309,625 - +0.6 3,309,625 - -0.5 3,309,625 - -0.4 3,309,864 + Pf1N1B4_2955 0.39 -0.0 3,309,873 + Pf1N1B4_2955 0.40 +1.8 3,309,873 + Pf1N1B4_2955 0.40 +1.1 3,309,873 + Pf1N1B4_2955 0.40 +0.9 3,309,873 + Pf1N1B4_2955 0.40 +2.3 3,309,873 + Pf1N1B4_2955 0.40 +1.0 3,309,874 - Pf1N1B4_2955 0.40 -0.7 3,309,874 - Pf1N1B4_2955 0.40 +1.1 3,310,086 + Pf1N1B4_2955 0.74 +0.7 3,310,086 + Pf1N1B4_2955 0.74 -0.2 3,310,087 - Pf1N1B4_2955 0.74 -0.1 3,310,087 - Pf1N1B4_2955 0.74 +0.8 3,310,087 - Pf1N1B4_2955 0.74 +0.1 3,310,087 - Pf1N1B4_2955 0.74 +0.1 3,310,259 + +0.8 3,310,259 + +0.3 3,310,259 + +0.2 3,310,259 + -0.3 3,310,259 + -1.1 3,310,260 - +0.1 3,310,382 + +1.1 3,310,382 + -0.2 3,310,382 + -0.9 3,310,382 + +0.1 3,310,382 + -0.9 3,310,383 - +0.7 3,310,383 - -0.9 3,310,383 - +0.5 3,310,383 - +0.0 3,310,383 - +2.1 3,310,383 - -0.7 3,310,383 - +0.2 3,310,383 - -1.7 3,310,383 - -2.5 3,310,383 - -1.1 3,310,383 - -0.3 3,310,478 - -0.4 3,310,478 - -0.2 3,310,478 - -2.1 3,310,478 - +0.4 3,310,486 + +1.7 3,310,486 + +1.1 3,310,486 + +0.5 3,310,486 + +0.9 3,310,486 + +0.3 3,310,487 - +2.4 3,310,487 - -1.7 3,310,487 - +1.7 3,310,487 - +2.1 3,310,492 + +0.1 3,310,493 - +0.7 3,310,528 + +0.3 3,310,528 + +0.1 3,310,528 + -1.1 3,310,528 + -0.1 3,310,528 + +2.3 3,310,529 - -0.7 3,310,529 - +0.0 3,310,529 - -0.5 3,310,529 - +3.4 3,310,529 - +1.1 3,310,569 - +1.4 3,310,640 + +0.1 3,310,853 + Pf1N1B4_2957 0.25 +0.1 3,310,853 + Pf1N1B4_2957 0.25 -0.7 3,310,853 + Pf1N1B4_2957 0.25 +0.5 3,310,913 + Pf1N1B4_2957 0.29 -1.2 3,310,913 + Pf1N1B4_2957 0.29 +0.2 3,310,913 + Pf1N1B4_2957 0.29 +1.1 3,310,913 + Pf1N1B4_2957 0.29 +0.7 3,310,914 - Pf1N1B4_2957 0.30 -1.5 3,310,914 - +1.4 3,311,012 + Pf1N1B4_2957 0.37 +0.3 3,311,013 - Pf1N1B4_2957 0.37 -0.1 3,311,013 - Pf1N1B4_2957 0.37 -0.1 3,311,045 + Pf1N1B4_2957 0.40 -1.5 3,311,046 - Pf1N1B4_2957 0.40 +0.3 3,311,090 + Pf1N1B4_2957 0.43 -1.9 3,311,090 + Pf1N1B4_2957 0.43 -0.5 3,311,090 + Pf1N1B4_2957 0.43 +0.6 3,311,091 - Pf1N1B4_2957 0.43 +0.4 3,311,126 + Pf1N1B4_2957 0.46 +0.4 3,311,126 + Pf1N1B4_2957 0.46 +2.1 3,311,126 + Pf1N1B4_2957 0.46 +1.9 3,311,127 - Pf1N1B4_2957 0.46 +0.5 3,311,127 - Pf1N1B4_2957 0.46 +1.9 3,311,222 + Pf1N1B4_2957 0.53 -0.4 3,311,222 + Pf1N1B4_2957 0.53 +0.3 3,311,222 + Pf1N1B4_2957 0.53 +0.5 3,311,222 + Pf1N1B4_2957 0.53 +0.1 3,311,222 + Pf1N1B4_2957 0.53 +0.7 3,311,222 + Pf1N1B4_2957 0.53 +0.1 3,311,222 + Pf1N1B4_2957 0.53 -0.2 3,311,222 + Pf1N1B4_2957 0.53 -0.1 3,311,222 + Pf1N1B4_2957 0.53 -0.1 3,311,222 + Pf1N1B4_2957 0.53 -1.5 3,311,222 + Pf1N1B4_2957 0.53 +0.1 3,311,223 - Pf1N1B4_2957 0.53 +1.1 3,311,223 - Pf1N1B4_2957 0.53 -0.5 3,311,223 - Pf1N1B4_2957 0.53 -1.7 3,311,223 - Pf1N1B4_2957 0.53 +0.1 3,311,223 - Pf1N1B4_2957 0.53 +2.4 3,311,223 - Pf1N1B4_2957 0.53 -0.5 3,311,223 - Pf1N1B4_2957 0.53 +1.1 3,311,223 - Pf1N1B4_2957 0.53 -1.7 3,311,223 - Pf1N1B4_2957 0.53 +0.5 3,311,223 - Pf1N1B4_2957 0.53 -0.5 3,311,223 - Pf1N1B4_2957 0.53 +1.1 3,311,223 - Pf1N1B4_2957 0.53 +1.3
Or see this region's nucleotide sequence