Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2709 and Pf1N1B4_2710 are separated by 5 nucleotides Pf1N1B4_2710 and Pf1N1B4_2711 overlap by 8 nucleotides Pf1N1B4_2711 and Pf1N1B4_2712 are separated by 214 nucleotides
Pf1N1B4_2709 - Magnesium and cobalt efflux protein CorC, at 3,047,156 to 3,047,995
_2709
Pf1N1B4_2710 - Metal-dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly, at 3,048,001 to 3,048,495
_2710
Pf1N1B4_2711 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 3,048,488 to 3,049,504
_2711
Pf1N1B4_2712 - tRNA-i(6)A37 methylthiotransferase, at 3,049,719 to 3,051,095
_2712
Position (kb)
3048
3049
3050 Strain fitness (log2 ratio)
-1
0
1
2 at 3047.807 kb on + strand, within Pf1N1B4_2709 at 3048.023 kb on - strand at 3048.838 kb on - strand, within Pf1N1B4_2711 at 3048.838 kb on - strand, within Pf1N1B4_2711 at 3048.889 kb on - strand, within Pf1N1B4_2711 at 3049.072 kb on - strand, within Pf1N1B4_2711 at 3049.072 kb on - strand, within Pf1N1B4_2711 at 3049.072 kb on - strand, within Pf1N1B4_2711 at 3049.138 kb on - strand at 3049.533 kb on - strand at 3049.579 kb on - strand at 3049.580 kb on + strand at 3049.660 kb on + strand at 3049.660 kb on + strand at 3050.048 kb on - strand, within Pf1N1B4_2712 at 3050.048 kb on - strand, within Pf1N1B4_2712 at 3050.192 kb on - strand, within Pf1N1B4_2712
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,047,807 + Pf1N1B4_2709 0.78 -0.6 3,048,023 - +1.0 3,048,838 - Pf1N1B4_2711 0.34 -0.8 3,048,838 - Pf1N1B4_2711 0.34 -1.4 3,048,889 - Pf1N1B4_2711 0.39 -0.1 3,049,072 - Pf1N1B4_2711 0.57 -0.6 3,049,072 - Pf1N1B4_2711 0.57 +0.4 3,049,072 - Pf1N1B4_2711 0.57 -0.4 3,049,138 - +0.2 3,049,533 - -0.4 3,049,579 - -0.0 3,049,580 + -1.6 3,049,660 + -1.1 3,049,660 + -0.8 3,050,048 - Pf1N1B4_2712 0.24 +1.8 3,050,048 - Pf1N1B4_2712 0.24 +0.9 3,050,192 - Pf1N1B4_2712 0.34 +1.2
Or see this region's nucleotide sequence