Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_24 and Pf1N1B4_25 overlap by 30 nucleotides Pf1N1B4_25 and Pf1N1B4_26 are separated by 257 nucleotides Pf1N1B4_26 and Pf1N1B4_27 are separated by 337 nucleotides
Pf1N1B4_24 - Glutamyl-tRNA synthetase (EC 6.1.1.17), at 26,536 to 27,849
_24
Pf1N1B4_25 - hypothetical protein, at 27,820 to 27,942
_25
Pf1N1B4_26 - Excinuclease ABC subunit B, at 28,200 to 30,215
_26
Pf1N1B4_27 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 30,553 to 31,749
_27
Position (kb)
28
29
30
31 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 27.941 kb on - strand at 28.007 kb on + strand at 28.007 kb on + strand at 28.007 kb on + strand at 28.011 kb on + strand at 28.012 kb on - strand at 28.029 kb on - strand at 28.064 kb on + strand at 28.064 kb on + strand at 28.064 kb on + strand at 28.122 kb on - strand at 28.355 kb on - strand at 29.023 kb on + strand, within Pf1N1B4_26 at 29.024 kb on - strand, within Pf1N1B4_26 at 29.166 kb on - strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.167 kb on + strand, within Pf1N1B4_26 at 29.168 kb on - strand, within Pf1N1B4_26 at 29.168 kb on - strand at 29.168 kb on - strand, within Pf1N1B4_26 at 29.168 kb on - strand, within Pf1N1B4_26 at 29.236 kb on + strand, within Pf1N1B4_26 at 29.401 kb on + strand, within Pf1N1B4_26 at 29.488 kb on + strand, within Pf1N1B4_26 at 29.616 kb on - strand at 29.677 kb on + strand, within Pf1N1B4_26 at 29.677 kb on + strand, within Pf1N1B4_26 at 29.677 kb on + strand, within Pf1N1B4_26 at 29.678 kb on - strand, within Pf1N1B4_26 at 29.758 kb on + strand, within Pf1N1B4_26 at 29.759 kb on - strand, within Pf1N1B4_26 at 29.782 kb on + strand, within Pf1N1B4_26 at 29.918 kb on - strand, within Pf1N1B4_26 at 29.936 kb on - strand, within Pf1N1B4_26 at 29.944 kb on + strand, within Pf1N1B4_26 at 29.945 kb on - strand, within Pf1N1B4_26 at 29.957 kb on - strand, within Pf1N1B4_26 at 29.998 kb on + strand, within Pf1N1B4_26 at 29.999 kb on - strand, within Pf1N1B4_26 at 29.999 kb on - strand, within Pf1N1B4_26 at 30.198 kb on + strand at 30.215 kb on + strand at 30.215 kb on + strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.216 kb on - strand at 30.219 kb on + strand at 30.219 kb on + strand at 30.219 kb on + strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.220 kb on - strand at 30.355 kb on - strand at 30.464 kb on + strand at 30.465 kb on - strand at 30.466 kb on + strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.467 kb on - strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.469 kb on + strand at 30.470 kb on - strand at 30.494 kb on - strand at 30.551 kb on + strand at 30.664 kb on + strand at 30.778 kb on + strand, within Pf1N1B4_27 at 31.006 kb on + strand, within Pf1N1B4_27 at 31.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 27,941 - +0.0 28,007 + +2.3 28,007 + -0.2 28,007 + -0.8 28,011 + -0.8 28,012 - -0.2 28,029 - +0.4 28,064 + -0.2 28,064 + +0.9 28,064 + -0.7 28,122 - -1.2 28,355 - +2.3 29,023 + Pf1N1B4_26 0.41 -0.9 29,024 - Pf1N1B4_26 0.41 +0.6 29,166 - Pf1N1B4_26 0.48 +0.5 29,167 + Pf1N1B4_26 0.48 +0.7 29,167 + Pf1N1B4_26 0.48 -1.6 29,167 + Pf1N1B4_26 0.48 +1.8 29,167 + Pf1N1B4_26 0.48 -0.6 29,167 + Pf1N1B4_26 0.48 +0.4 29,168 - Pf1N1B4_26 0.48 +0.3 29,168 - -1.2 29,168 - Pf1N1B4_26 0.48 +1.4 29,168 - Pf1N1B4_26 0.48 -1.6 29,236 + Pf1N1B4_26 0.51 +2.0 29,401 + Pf1N1B4_26 0.60 -0.2 29,488 + Pf1N1B4_26 0.64 -1.2 29,616 - -0.8 29,677 + Pf1N1B4_26 0.73 -0.2 29,677 + Pf1N1B4_26 0.73 +2.0 29,677 + Pf1N1B4_26 0.73 -1.8 29,678 - Pf1N1B4_26 0.73 +0.4 29,758 + Pf1N1B4_26 0.77 +0.8 29,759 - Pf1N1B4_26 0.77 +0.1 29,782 + Pf1N1B4_26 0.78 +1.0 29,918 - Pf1N1B4_26 0.85 +1.7 29,936 - Pf1N1B4_26 0.86 +0.5 29,944 + Pf1N1B4_26 0.87 +1.3 29,945 - Pf1N1B4_26 0.87 -0.1 29,957 - Pf1N1B4_26 0.87 +3.3 29,998 + Pf1N1B4_26 0.89 +1.0 29,999 - Pf1N1B4_26 0.89 +1.3 29,999 - Pf1N1B4_26 0.89 +1.3 30,198 + -1.2 30,215 + +1.2 30,215 + +2.2 30,216 - -0.2 30,216 - -0.1 30,216 - -0.2 30,216 - +1.2 30,216 - +2.6 30,216 - +0.0 30,216 - +0.3 30,216 - -0.2 30,216 - +1.3 30,219 + +1.0 30,219 + +1.8 30,219 + -0.9 30,220 - -0.4 30,220 - +1.3 30,220 - -1.1 30,220 - +0.2 30,220 - -0.8 30,220 - +1.3 30,220 - -2.1 30,220 - -0.9 30,220 - +1.3 30,355 - -1.2 30,464 + -1.6 30,465 - +0.8 30,466 + -0.6 30,467 - +1.0 30,467 - +1.0 30,467 - -0.0 30,467 - +0.5 30,469 + +0.8 30,469 + +1.9 30,469 + +1.3 30,469 + +0.2 30,469 + -2.4 30,469 + -0.2 30,469 + -0.4 30,470 - +0.8 30,494 - -1.2 30,551 + -0.2 30,664 + -0.8 30,778 + Pf1N1B4_27 0.19 +0.5 31,006 + Pf1N1B4_27 0.38 +0.0 31,007 - -0.2
Or see this region's nucleotide sequence