Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2479 and Pf1N1B4_2480 are separated by 214 nucleotides Pf1N1B4_2480 and Pf1N1B4_2481 are separated by 162 nucleotides
Pf1N1B4_2479 - Glycine cleavage system transcriptional antiactivator GcvR, at 2,813,174 to 2,813,692
_2479
Pf1N1B4_2480 - Protein rarD, at 2,813,907 to 2,814,794
_2480
Pf1N1B4_2481 - YihE protein, required for LPS synthesis, at 2,814,957 to 2,815,931
_2481
Position (kb)
2813
2814
2815 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2812.925 kb on + strand at 2812.925 kb on + strand at 2812.926 kb on - strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.931 kb on + strand at 2812.932 kb on - strand at 2813.017 kb on - strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.128 kb on + strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.129 kb on - strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.130 kb on + strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.131 kb on - strand at 2813.143 kb on - strand at 2813.143 kb on - strand at 2813.188 kb on - strand at 2813.339 kb on + strand, within Pf1N1B4_2479 at 2813.352 kb on + strand, within Pf1N1B4_2479 at 2813.353 kb on - strand, within Pf1N1B4_2479 at 2813.541 kb on + strand, within Pf1N1B4_2479 at 2813.541 kb on + strand, within Pf1N1B4_2479 at 2813.542 kb on - strand, within Pf1N1B4_2479 at 2813.699 kb on - strand at 2813.707 kb on + strand at 2813.709 kb on + strand at 2813.709 kb on + strand at 2813.709 kb on + strand at 2813.709 kb on + strand at 2813.710 kb on - strand at 2813.710 kb on - strand at 2813.710 kb on - strand at 2813.929 kb on - strand at 2813.948 kb on - strand at 2813.948 kb on - strand at 2813.969 kb on + strand at 2813.969 kb on + strand at 2813.969 kb on + strand at 2813.970 kb on - strand at 2814.056 kb on - strand, within Pf1N1B4_2480 at 2814.056 kb on - strand, within Pf1N1B4_2480 at 2814.056 kb on - strand, within Pf1N1B4_2480 at 2814.079 kb on + strand, within Pf1N1B4_2480 at 2814.079 kb on + strand, within Pf1N1B4_2480 at 2814.080 kb on - strand, within Pf1N1B4_2480 at 2814.305 kb on - strand at 2814.305 kb on - strand, within Pf1N1B4_2480 at 2814.305 kb on - strand, within Pf1N1B4_2480 at 2814.363 kb on + strand, within Pf1N1B4_2480 at 2814.364 kb on - strand, within Pf1N1B4_2480 at 2814.364 kb on - strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.475 kb on + strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.476 kb on - strand, within Pf1N1B4_2480 at 2814.479 kb on - strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.526 kb on + strand, within Pf1N1B4_2480 at 2814.527 kb on - strand at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.527 kb on - strand, within Pf1N1B4_2480 at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.719 kb on - strand at 2814.745 kb on + strand at 2814.745 kb on + strand at 2814.745 kb on + strand at 2814.745 kb on + strand at 2814.745 kb on + strand at 2814.746 kb on - strand at 2814.746 kb on - strand at 2814.746 kb on - strand at 2814.766 kb on + strand at 2814.809 kb on - strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.833 kb on + strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.834 kb on - strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.835 kb on + strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.836 kb on - strand at 2814.856 kb on - strand at 2814.941 kb on - strand at 2814.959 kb on - strand at 2814.959 kb on - strand at 2815.106 kb on - strand, within Pf1N1B4_2481 at 2815.106 kb on - strand, within Pf1N1B4_2481 at 2815.106 kb on - strand, within Pf1N1B4_2481 at 2815.180 kb on + strand, within Pf1N1B4_2481 at 2815.180 kb on + strand, within Pf1N1B4_2481 at 2815.248 kb on - strand, within Pf1N1B4_2481 at 2815.248 kb on - strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.375 kb on + strand, within Pf1N1B4_2481 at 2815.376 kb on - strand, within Pf1N1B4_2481 at 2815.376 kb on - strand, within Pf1N1B4_2481 at 2815.574 kb on - strand, within Pf1N1B4_2481
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 2,812,925 + -0.8 2,812,925 + +1.5 2,812,926 - -1.1 2,812,931 + +0.9 2,812,931 + +0.8 2,812,931 + +0.4 2,812,931 + -0.6 2,812,932 - +1.8 2,813,017 - +0.9 2,813,128 + -0.9 2,813,128 + -1.6 2,813,128 + -1.4 2,813,128 + +1.2 2,813,129 - +1.2 2,813,129 - +0.7 2,813,129 - -1.1 2,813,129 - -0.3 2,813,129 - +0.2 2,813,129 - -2.1 2,813,129 - +0.0 2,813,129 - -1.1 2,813,130 + -0.3 2,813,130 + +1.1 2,813,130 + -1.6 2,813,130 + +2.2 2,813,130 + +1.1 2,813,130 + +0.4 2,813,130 + -0.5 2,813,130 + -0.4 2,813,130 + +0.8 2,813,131 - +0.2 2,813,131 - +0.2 2,813,131 - +1.2 2,813,131 - -0.2 2,813,131 - +1.1 2,813,131 - -0.8 2,813,131 - +0.4 2,813,131 - +1.1 2,813,143 - -0.2 2,813,143 - +1.6 2,813,188 - -1.4 2,813,339 + Pf1N1B4_2479 0.32 -0.6 2,813,352 + Pf1N1B4_2479 0.34 +1.2 2,813,353 - Pf1N1B4_2479 0.34 -1.4 2,813,541 + Pf1N1B4_2479 0.71 -0.6 2,813,541 + Pf1N1B4_2479 0.71 -0.4 2,813,542 - Pf1N1B4_2479 0.71 -0.8 2,813,699 - +0.5 2,813,707 + +0.0 2,813,709 + +0.2 2,813,709 + +0.2 2,813,709 + -1.5 2,813,709 + +0.2 2,813,710 - +0.1 2,813,710 - -0.4 2,813,710 - +1.8 2,813,929 - +1.2 2,813,948 - -1.1 2,813,948 - -2.4 2,813,969 + +0.6 2,813,969 + +0.2 2,813,969 + +1.2 2,813,970 - -1.8 2,814,056 - Pf1N1B4_2480 0.17 +1.0 2,814,056 - Pf1N1B4_2480 0.17 +0.1 2,814,056 - Pf1N1B4_2480 0.17 +1.2 2,814,079 + Pf1N1B4_2480 0.19 +0.2 2,814,079 + Pf1N1B4_2480 0.19 +2.0 2,814,080 - Pf1N1B4_2480 0.19 -0.5 2,814,305 - +0.2 2,814,305 - Pf1N1B4_2480 0.45 +0.2 2,814,305 - Pf1N1B4_2480 0.45 -0.3 2,814,363 + Pf1N1B4_2480 0.51 -0.8 2,814,364 - Pf1N1B4_2480 0.51 +1.9 2,814,364 - Pf1N1B4_2480 0.51 -0.8 2,814,475 + Pf1N1B4_2480 0.64 +0.4 2,814,475 + Pf1N1B4_2480 0.64 -0.7 2,814,475 + Pf1N1B4_2480 0.64 +0.2 2,814,475 + Pf1N1B4_2480 0.64 -0.4 2,814,475 + Pf1N1B4_2480 0.64 -0.2 2,814,476 - Pf1N1B4_2480 0.64 +0.2 2,814,476 - Pf1N1B4_2480 0.64 +1.6 2,814,476 - Pf1N1B4_2480 0.64 -0.8 2,814,476 - Pf1N1B4_2480 0.64 +0.2 2,814,476 - Pf1N1B4_2480 0.64 -0.1 2,814,476 - Pf1N1B4_2480 0.64 -1.1 2,814,476 - Pf1N1B4_2480 0.64 -1.1 2,814,479 - Pf1N1B4_2480 0.64 +0.7 2,814,526 + Pf1N1B4_2480 0.70 +0.5 2,814,526 + Pf1N1B4_2480 0.70 -0.8 2,814,526 + Pf1N1B4_2480 0.70 +0.2 2,814,526 + Pf1N1B4_2480 0.70 +1.0 2,814,526 + Pf1N1B4_2480 0.70 -2.1 2,814,526 + Pf1N1B4_2480 0.70 +0.8 2,814,526 + Pf1N1B4_2480 0.70 +0.1 2,814,526 + Pf1N1B4_2480 0.70 -0.1 2,814,526 + Pf1N1B4_2480 0.70 -0.4 2,814,526 + Pf1N1B4_2480 0.70 -1.9 2,814,527 - +0.2 2,814,527 - Pf1N1B4_2480 0.70 -1.1 2,814,527 - Pf1N1B4_2480 0.70 -1.1 2,814,527 - Pf1N1B4_2480 0.70 -1.8 2,814,527 - Pf1N1B4_2480 0.70 -0.8 2,814,527 - Pf1N1B4_2480 0.70 +0.2 2,814,527 - Pf1N1B4_2480 0.70 -0.2 2,814,527 - Pf1N1B4_2480 0.70 +0.8 2,814,527 - Pf1N1B4_2480 0.70 +1.2 2,814,527 - Pf1N1B4_2480 0.70 +1.0 2,814,527 - Pf1N1B4_2480 0.70 +0.2 2,814,719 - +0.2 2,814,719 - -1.5 2,814,719 - +0.4 2,814,719 - +2.0 2,814,719 - -1.1 2,814,719 - +0.0 2,814,745 + +0.6 2,814,745 + -0.8 2,814,745 + +0.8 2,814,745 + -1.2 2,814,745 + -1.1 2,814,746 - +0.7 2,814,746 - -0.8 2,814,746 - +0.8 2,814,766 + -0.1 2,814,809 - +0.0 2,814,833 + +1.2 2,814,833 + +1.8 2,814,833 + -0.6 2,814,833 + -0.2 2,814,833 + -0.8 2,814,833 + -0.4 2,814,834 - +0.2 2,814,834 - -0.5 2,814,834 - -0.2 2,814,834 - +1.5 2,814,834 - +1.2 2,814,834 - -0.7 2,814,834 - -1.4 2,814,834 - +0.8 2,814,834 - -0.8 2,814,834 - +2.2 2,814,835 + +0.2 2,814,835 + +0.0 2,814,835 + +1.0 2,814,835 + +0.5 2,814,835 + +1.6 2,814,835 + +0.6 2,814,835 + +1.6 2,814,835 + -0.5 2,814,836 - +0.5 2,814,836 - +2.0 2,814,836 - +1.0 2,814,836 - +1.2 2,814,836 - +0.7 2,814,836 - +0.2 2,814,836 - -0.4 2,814,836 - +1.6 2,814,836 - +2.5 2,814,836 - +0.6 2,814,836 - +0.8 2,814,836 - +0.4 2,814,836 - -0.8 2,814,856 - -0.5 2,814,941 - -0.9 2,814,959 - +0.3 2,814,959 - -1.1 2,815,106 - Pf1N1B4_2481 0.15 +0.2 2,815,106 - Pf1N1B4_2481 0.15 -0.4 2,815,106 - Pf1N1B4_2481 0.15 -1.4 2,815,180 + Pf1N1B4_2481 0.23 -0.0 2,815,180 + Pf1N1B4_2481 0.23 +0.0 2,815,248 - Pf1N1B4_2481 0.30 -0.3 2,815,248 - Pf1N1B4_2481 0.30 -1.6 2,815,375 + Pf1N1B4_2481 0.43 -1.1 2,815,375 + Pf1N1B4_2481 0.43 -0.2 2,815,375 + Pf1N1B4_2481 0.43 +1.2 2,815,375 + Pf1N1B4_2481 0.43 +0.2 2,815,375 + Pf1N1B4_2481 0.43 +3.2 2,815,375 + Pf1N1B4_2481 0.43 -1.5 2,815,375 + Pf1N1B4_2481 0.43 +0.2 2,815,376 - Pf1N1B4_2481 0.43 -0.4 2,815,376 - Pf1N1B4_2481 0.43 +0.5 2,815,574 - Pf1N1B4_2481 0.63 +0.2
Or see this region's nucleotide sequence