Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_239 and Pf1N1B4_240 are separated by 73 nucleotides Pf1N1B4_240 and Pf1N1B4_241 are separated by 163 nucleotides Pf1N1B4_241 and Pf1N1B4_242 are separated by 192 nucleotides
Pf1N1B4_239 - Similar to ribosomal large subunit pseudouridine synthase A, at 298,039 to 298,929
_239
Pf1N1B4_240 - Transcriptional regulators, at 299,003 to 299,323
_240
Pf1N1B4_241 - Thiol-disulfide isomerase and thioredoxins, at 299,487 to 299,744
_241
Pf1N1B4_242 - Glutathione-regulated potassium-efflux system protein KefB, at 299,937 to 301,649
_242
Position (kb) 299
300 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 298.011 kb on - strand at 298.011 kb on - strand at 298.140 kb on - strand, within Pf1N1B4_239 at 298.140 kb on - strand, within Pf1N1B4_239 at 298.140 kb on - strand, within Pf1N1B4_239 at 298.151 kb on - strand, within Pf1N1B4_239 at 298.251 kb on - strand, within Pf1N1B4_239 at 298.360 kb on - strand, within Pf1N1B4_239 at 298.372 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.713 kb on - strand, within Pf1N1B4_239 at 298.736 kb on + strand, within Pf1N1B4_239 at 298.737 kb on - strand at 298.880 kb on + strand at 298.880 kb on + strand at 298.881 kb on - strand at 298.881 kb on - strand at 298.953 kb on - strand at 298.990 kb on + strand at 298.990 kb on + strand at 298.991 kb on - strand at 298.991 kb on - strand at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.103 kb on + strand, within Pf1N1B4_240 at 299.189 kb on - strand, within Pf1N1B4_240 at 299.357 kb on + strand at 299.411 kb on - strand at 299.576 kb on + strand, within Pf1N1B4_241 at 299.587 kb on + strand, within Pf1N1B4_241 at 299.750 kb on + strand at 299.750 kb on + strand at 299.750 kb on + strand at 299.751 kb on - strand at 299.751 kb on - strand at 299.763 kb on + strand at 299.764 kb on - strand at 299.798 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.900 kb on + strand at 299.901 kb on - strand at 299.901 kb on - strand at 299.901 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 298,011 - +2.4 298,011 - +0.4 298,140 - Pf1N1B4_239 0.11 +0.3 298,140 - Pf1N1B4_239 0.11 +0.1 298,140 - Pf1N1B4_239 0.11 +0.2 298,151 - Pf1N1B4_239 0.13 -1.4 298,251 - Pf1N1B4_239 0.24 -0.1 298,360 - Pf1N1B4_239 0.36 +0.4 298,372 - Pf1N1B4_239 0.37 +1.8 298,713 - Pf1N1B4_239 0.76 -1.8 298,713 - Pf1N1B4_239 0.76 -2.4 298,713 - Pf1N1B4_239 0.76 +0.2 298,736 + Pf1N1B4_239 0.78 -2.4 298,737 - -0.8 298,880 + +0.8 298,880 + -0.8 298,881 - +0.3 298,881 - -2.2 298,953 - +1.4 298,990 + +1.8 298,990 + +0.5 298,991 - -0.2 298,991 - -0.4 299,103 + Pf1N1B4_240 0.31 -0.8 299,103 + Pf1N1B4_240 0.31 -0.2 299,103 + Pf1N1B4_240 0.31 -0.7 299,189 - Pf1N1B4_240 0.58 -1.8 299,357 + -1.2 299,411 - -2.0 299,576 + Pf1N1B4_241 0.34 +1.0 299,587 + Pf1N1B4_241 0.39 -1.2 299,750 + +1.3 299,750 + +0.8 299,750 + -0.1 299,751 - +4.0 299,751 - -2.0 299,763 + +1.8 299,764 - -0.6 299,798 + +0.8 299,900 + +0.4 299,900 + +0.4 299,900 + +1.3 299,900 + -1.8 299,900 + -0.7 299,901 - +0.6 299,901 - +1.8 299,901 - +2.1
Or see this region's nucleotide sequence