Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_232

Experiment: Nalidixic 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_231 and Pf1N1B4_232 are separated by 47 nucleotidesPf1N1B4_232 and Pf1N1B4_233 overlap by 17 nucleotides Pf1N1B4_231 - Sensor histidine kinase, at 292,071 to 292,964 _231 Pf1N1B4_232 - DNA-binding response regulator, LuxR family, at 293,012 to 293,620 _232 Pf1N1B4_233 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 293,604 to 294,536 _233 Position (kb) 293 294Strain fitness (log2 ratio) -2 -1 0 1 2at 292.070 kb on + strandat 292.071 kb on - strandat 292.074 kb on + strandat 292.075 kb on - strandat 292.075 kb on - strandat 292.095 kb on - strandat 292.095 kb on - strandat 292.095 kb on - strandat 292.148 kb on + strandat 292.148 kb on + strandat 292.149 kb on - strandat 292.193 kb on - strand, within Pf1N1B4_231at 292.224 kb on - strand, within Pf1N1B4_231at 292.561 kb on + strand, within Pf1N1B4_231at 292.561 kb on + strandat 292.562 kb on - strand, within Pf1N1B4_231at 292.580 kb on + strand, within Pf1N1B4_231at 292.580 kb on + strand, within Pf1N1B4_231at 292.580 kb on + strand, within Pf1N1B4_231at 292.581 kb on - strand, within Pf1N1B4_231at 292.682 kb on + strand, within Pf1N1B4_231at 292.682 kb on + strand, within Pf1N1B4_231at 292.683 kb on - strandat 292.684 kb on + strand, within Pf1N1B4_231at 292.684 kb on + strand, within Pf1N1B4_231at 292.684 kb on + strand, within Pf1N1B4_231at 292.706 kb on + strand, within Pf1N1B4_231at 292.849 kb on + strand, within Pf1N1B4_231at 292.849 kb on + strand, within Pf1N1B4_231at 292.931 kb on - strandat 292.949 kb on + strandat 292.950 kb on - strandat 292.969 kb on + strandat 292.969 kb on + strandat 292.970 kb on - strandat 292.974 kb on + strandat 292.974 kb on + strandat 292.974 kb on + strandat 292.974 kb on + strandat 292.975 kb on - strandat 292.975 kb on - strandat 292.999 kb on + strandat 293.049 kb on + strandat 293.125 kb on + strandat 293.558 kb on + strand, within Pf1N1B4_232at 293.559 kb on - strand, within Pf1N1B4_232at 293.559 kb on - strand, within Pf1N1B4_232at 293.735 kb on + strand, within Pf1N1B4_233at 293.736 kb on - strand, within Pf1N1B4_233at 293.736 kb on - strandat 293.737 kb on + strandat 293.738 kb on - strandat 293.738 kb on - strand, within Pf1N1B4_233at 293.738 kb on - strand, within Pf1N1B4_233at 293.738 kb on - strand, within Pf1N1B4_233at 293.738 kb on - strand, within Pf1N1B4_233at 293.738 kb on - strandat 293.738 kb on - strand, within Pf1N1B4_233at 294.046 kb on + strand, within Pf1N1B4_233at 294.046 kb on + strand, within Pf1N1B4_233at 294.047 kb on - strand, within Pf1N1B4_233at 294.087 kb on - strand, within Pf1N1B4_233at 294.087 kb on - strand, within Pf1N1B4_233at 294.087 kb on - strand, within Pf1N1B4_233at 294.264 kb on + strand, within Pf1N1B4_233at 294.265 kb on - strandat 294.351 kb on - strand, within Pf1N1B4_233at 294.555 kb on + strandat 294.555 kb on + strandat 294.555 kb on + strandat 294.556 kb on - strandat 294.556 kb on - strandat 294.578 kb on + strandat 294.579 kb on - strandat 294.579 kb on - strandat 294.600 kb on + strandat 294.601 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml
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292,070 + +0.3
292,071 - -0.8
292,074 + -0.2
292,075 - -1.5
292,075 - +2.1
292,095 - +0.2
292,095 - +0.8
292,095 - -0.2
292,148 + +0.8
292,148 + -1.4
292,149 - +0.8
292,193 - Pf1N1B4_231 0.14 -1.8
292,224 - Pf1N1B4_231 0.17 +0.8
292,561 + Pf1N1B4_231 0.55 +1.0
292,561 + -0.2
292,562 - Pf1N1B4_231 0.55 +1.1
292,580 + Pf1N1B4_231 0.57 +0.4
292,580 + Pf1N1B4_231 0.57 +0.8
292,580 + Pf1N1B4_231 0.57 -1.8
292,581 - Pf1N1B4_231 0.57 +1.0
292,682 + Pf1N1B4_231 0.68 -0.5
292,682 + Pf1N1B4_231 0.68 -2.5
292,683 - -0.8
292,684 + Pf1N1B4_231 0.69 +0.1
292,684 + Pf1N1B4_231 0.69 +0.9
292,684 + Pf1N1B4_231 0.69 -0.2
292,706 + Pf1N1B4_231 0.71 -0.8
292,849 + Pf1N1B4_231 0.87 +0.3
292,849 + Pf1N1B4_231 0.87 -0.4
292,931 - +0.5
292,949 + -0.4
292,950 - -0.5
292,969 + -2.2
292,969 + +0.2
292,970 - +0.2
292,974 + +0.4
292,974 + +2.5
292,974 + -0.3
292,974 + +0.7
292,975 - -0.8
292,975 - -0.1
292,999 + -0.4
293,049 + -0.2
293,125 + -0.8
293,558 + Pf1N1B4_232 0.90 -0.2
293,559 - Pf1N1B4_232 0.90 -0.5
293,559 - Pf1N1B4_232 0.90 -1.8
293,735 + Pf1N1B4_233 0.14 -0.2
293,736 - Pf1N1B4_233 0.14 -1.2
293,736 - -0.2
293,737 + +0.8
293,738 - +0.8
293,738 - Pf1N1B4_233 0.14 +0.5
293,738 - Pf1N1B4_233 0.14 -0.5
293,738 - Pf1N1B4_233 0.14 +0.3
293,738 - Pf1N1B4_233 0.14 -0.5
293,738 - +1.4
293,738 - Pf1N1B4_233 0.14 -0.8
294,046 + Pf1N1B4_233 0.47 +1.0
294,046 + Pf1N1B4_233 0.47 +0.8
294,047 - Pf1N1B4_233 0.47 +0.8
294,087 - Pf1N1B4_233 0.52 +1.2
294,087 - Pf1N1B4_233 0.52 -0.8
294,087 - Pf1N1B4_233 0.52 -0.0
294,264 + Pf1N1B4_233 0.71 +0.8
294,265 - -0.2
294,351 - Pf1N1B4_233 0.80 +0.8
294,555 + -1.0
294,555 + -2.4
294,555 + +0.5
294,556 - -0.6
294,556 - +0.7
294,578 + -2.0
294,579 - +0.1
294,579 - +1.5
294,600 + +1.8
294,601 - +0.5

Or see this region's nucleotide sequence