Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_220 and Pf1N1B4_221 are separated by 12 nucleotides Pf1N1B4_221 and Pf1N1B4_222 are separated by 2 nucleotides
Pf1N1B4_220 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 279,272 to 281,074
_220
Pf1N1B4_221 - Citronellol and citronellal dehydrogenase, at 281,087 to 282,001
_221
Pf1N1B4_222 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 282,004 to 283,620
_222
Position (kb) 281
282
283 Strain fitness (log2 ratio) -2
-1
0
1
2 at 280.094 kb on + strand, within Pf1N1B4_220 at 280.095 kb on - strand, within Pf1N1B4_220 at 280.202 kb on + strand at 280.203 kb on - strand, within Pf1N1B4_220 at 280.204 kb on + strand, within Pf1N1B4_220 at 280.205 kb on - strand, within Pf1N1B4_220 at 280.205 kb on - strand, within Pf1N1B4_220 at 280.395 kb on - strand, within Pf1N1B4_220 at 280.395 kb on - strand, within Pf1N1B4_220 at 280.666 kb on - strand, within Pf1N1B4_220 at 280.666 kb on - strand, within Pf1N1B4_220 at 280.666 kb on - strand, within Pf1N1B4_220 at 280.734 kb on + strand, within Pf1N1B4_220 at 280.817 kb on + strand, within Pf1N1B4_220 at 280.818 kb on - strand, within Pf1N1B4_220 at 280.913 kb on + strand at 280.914 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.073 kb on - strand at 281.093 kb on + strand at 281.093 kb on + strand at 281.394 kb on - strand, within Pf1N1B4_221 at 281.439 kb on + strand, within Pf1N1B4_221 at 281.439 kb on + strand, within Pf1N1B4_221 at 281.440 kb on - strand, within Pf1N1B4_221 at 281.693 kb on + strand, within Pf1N1B4_221 at 281.693 kb on + strand, within Pf1N1B4_221 at 281.693 kb on + strand at 281.693 kb on + strand, within Pf1N1B4_221 at 281.694 kb on - strand, within Pf1N1B4_221 at 281.694 kb on - strand, within Pf1N1B4_221 at 281.740 kb on - strand, within Pf1N1B4_221 at 281.740 kb on - strand, within Pf1N1B4_221 at 281.903 kb on + strand, within Pf1N1B4_221 at 281.904 kb on - strand, within Pf1N1B4_221 at 281.904 kb on - strand, within Pf1N1B4_221 at 281.924 kb on + strand at 281.925 kb on - strand at 281.925 kb on - strand at 281.928 kb on + strand at 281.937 kb on + strand at 281.938 kb on - strand at 282.181 kb on - strand, within Pf1N1B4_222 at 282.181 kb on - strand, within Pf1N1B4_222 at 282.463 kb on + strand, within Pf1N1B4_222 at 282.464 kb on - strand at 282.601 kb on + strand, within Pf1N1B4_222 at 282.602 kb on - strand, within Pf1N1B4_222 at 282.739 kb on + strand, within Pf1N1B4_222 at 282.739 kb on + strand, within Pf1N1B4_222 at 282.739 kb on + strand, within Pf1N1B4_222
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 280,094 + Pf1N1B4_220 0.46 +1.3 280,095 - Pf1N1B4_220 0.46 +0.3 280,202 + +1.5 280,203 - Pf1N1B4_220 0.52 +2.4 280,204 + Pf1N1B4_220 0.52 +2.0 280,205 - Pf1N1B4_220 0.52 +1.0 280,205 - Pf1N1B4_220 0.52 +0.5 280,395 - Pf1N1B4_220 0.62 -0.8 280,395 - Pf1N1B4_220 0.62 -0.8 280,666 - Pf1N1B4_220 0.77 -0.2 280,666 - Pf1N1B4_220 0.77 +0.9 280,666 - Pf1N1B4_220 0.77 +0.8 280,734 + Pf1N1B4_220 0.81 +0.2 280,817 + Pf1N1B4_220 0.86 -1.4 280,818 - Pf1N1B4_220 0.86 -0.1 280,913 + -1.2 280,914 - +1.4 281,073 - +1.4 281,073 - +1.4 281,073 - -0.2 281,073 - -1.2 281,073 - -0.8 281,093 + +0.8 281,093 + -1.2 281,394 - Pf1N1B4_221 0.34 +0.2 281,439 + Pf1N1B4_221 0.38 +0.8 281,439 + Pf1N1B4_221 0.38 +0.4 281,440 - Pf1N1B4_221 0.39 +1.5 281,693 + Pf1N1B4_221 0.66 +1.2 281,693 + Pf1N1B4_221 0.66 +0.3 281,693 + -0.2 281,693 + Pf1N1B4_221 0.66 +0.8 281,694 - Pf1N1B4_221 0.66 -1.8 281,694 - Pf1N1B4_221 0.66 +0.3 281,740 - Pf1N1B4_221 0.71 +0.5 281,740 - Pf1N1B4_221 0.71 +0.3 281,903 + Pf1N1B4_221 0.89 -0.5 281,904 - Pf1N1B4_221 0.89 +1.4 281,904 - Pf1N1B4_221 0.89 +1.4 281,924 + -0.4 281,925 - -0.4 281,925 - +0.8 281,928 + -0.8 281,937 + -1.4 281,938 - -2.2 282,181 - Pf1N1B4_222 0.11 -0.2 282,181 - Pf1N1B4_222 0.11 +0.6 282,463 + Pf1N1B4_222 0.28 +0.8 282,464 - -0.8 282,601 + Pf1N1B4_222 0.37 +1.2 282,602 - Pf1N1B4_222 0.37 -0.1 282,739 + Pf1N1B4_222 0.45 +0.8 282,739 + Pf1N1B4_222 0.45 +1.8 282,739 + Pf1N1B4_222 0.45 +0.1
Or see this region's nucleotide sequence