Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2169 and Pf1N1B4_2170 are separated by 162 nucleotides Pf1N1B4_2170 and Pf1N1B4_2171 overlap by 4 nucleotides Pf1N1B4_2171 and Pf1N1B4_2172 are separated by 2 nucleotides Pf1N1B4_2172 and Pf1N1B4_2173 are separated by 13 nucleotides
Pf1N1B4_2169 - FIG00953808: hypothetical protein, at 2,483,135 to 2,483,458
_2169
Pf1N1B4_2170 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 2,483,621 to 2,484,322
_2170
Pf1N1B4_2171 - Tyrosine recombinase XerC, at 2,484,319 to 2,485,218
_2171
Pf1N1B4_2172 - Protein of unknown function DUF484, at 2,485,221 to 2,485,946
_2172
Pf1N1B4_2173 - Diaminopimelate epimerase (EC 5.1.1.7), at 2,485,960 to 2,486,790
_2173
Position (kb)
2484
2485
2486 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2483.432 kb on - strand at 2483.438 kb on + strand at 2483.439 kb on - strand at 2483.439 kb on - strand at 2483.439 kb on - strand at 2483.452 kb on + strand at 2483.452 kb on + strand at 2483.452 kb on + strand at 2483.452 kb on + strand at 2483.453 kb on - strand at 2483.453 kb on - strand at 2483.453 kb on - strand at 2483.453 kb on - strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.456 kb on + strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.457 kb on - strand at 2483.553 kb on + strand at 2483.553 kb on + strand at 2483.553 kb on + strand at 2483.553 kb on + strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.554 kb on - strand at 2483.558 kb on + strand at 2483.558 kb on + strand at 2483.558 kb on + strand at 2483.559 kb on - strand at 2483.559 kb on - strand at 2483.569 kb on + strand at 2483.569 kb on + strand at 2483.569 kb on + strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.570 kb on - strand at 2483.577 kb on + strand at 2483.578 kb on - strand at 2483.623 kb on - strand at 2483.623 kb on - strand at 2483.623 kb on - strand at 2483.623 kb on - strand at 2483.629 kb on + strand at 2483.629 kb on + strand at 2483.630 kb on - strand at 2483.630 kb on - strand at 2483.668 kb on + strand at 2483.705 kb on + strand, within Pf1N1B4_2170 at 2483.706 kb on - strand, within Pf1N1B4_2170 at 2483.788 kb on + strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.789 kb on - strand, within Pf1N1B4_2170 at 2483.899 kb on - strand, within Pf1N1B4_2170 at 2483.951 kb on + strand, within Pf1N1B4_2170 at 2483.952 kb on - strand, within Pf1N1B4_2170 at 2483.952 kb on - strand, within Pf1N1B4_2170 at 2483.958 kb on + strand, within Pf1N1B4_2170 at 2483.958 kb on + strand, within Pf1N1B4_2170 at 2484.070 kb on + strand, within Pf1N1B4_2170 at 2484.070 kb on + strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.071 kb on - strand, within Pf1N1B4_2170 at 2484.336 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.405 kb on - strand at 2484.405 kb on - strand at 2484.405 kb on - strand at 2484.797 kb on + strand, within Pf1N1B4_2171 at 2484.798 kb on - strand, within Pf1N1B4_2171 at 2484.798 kb on - strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.977 kb on + strand, within Pf1N1B4_2171 at 2484.977 kb on + strand, within Pf1N1B4_2171 at 2484.978 kb on - strand, within Pf1N1B4_2171 at 2485.148 kb on + strand at 2485.227 kb on + strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.319 kb on - strand at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.626 kb on + strand, within Pf1N1B4_2172 at 2485.626 kb on + strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.918 kb on - strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.983 kb on - strand at 2485.983 kb on - strand at 2485.983 kb on - strand at 2486.071 kb on + strand, within Pf1N1B4_2173
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 2,483,432 - -2.2 2,483,438 + +0.7 2,483,439 - +1.4 2,483,439 - +1.4 2,483,439 - +2.1 2,483,452 + +1.0 2,483,452 + -0.6 2,483,452 + -0.6 2,483,452 + +1.5 2,483,453 - +1.4 2,483,453 - -0.2 2,483,453 - -0.1 2,483,453 - -0.5 2,483,456 + -0.6 2,483,456 + +1.4 2,483,456 + -0.2 2,483,456 + +0.4 2,483,456 + +0.0 2,483,456 + -0.2 2,483,456 + +0.8 2,483,456 + +0.8 2,483,456 + +1.4 2,483,456 + +0.5 2,483,456 + +0.3 2,483,456 + +1.0 2,483,457 - +0.8 2,483,457 - +0.2 2,483,457 - +1.8 2,483,457 - +1.4 2,483,457 - -1.2 2,483,457 - -0.2 2,483,457 - +0.9 2,483,457 - +0.9 2,483,457 - +2.1 2,483,457 - -1.1 2,483,457 - -0.6 2,483,553 + -0.6 2,483,553 + +0.4 2,483,553 + +1.4 2,483,553 + +0.4 2,483,554 - +0.2 2,483,554 - +0.0 2,483,554 - +0.6 2,483,554 - -0.6 2,483,554 - +2.2 2,483,554 - -0.2 2,483,554 - -0.8 2,483,558 + -0.4 2,483,558 + -0.2 2,483,558 + +0.6 2,483,559 - -0.9 2,483,559 - -0.2 2,483,569 + +0.4 2,483,569 + -2.1 2,483,569 + -1.4 2,483,570 - -0.2 2,483,570 - -1.0 2,483,570 - -1.1 2,483,570 - +0.4 2,483,570 - +0.6 2,483,570 - -1.4 2,483,570 - -0.6 2,483,577 + +0.2 2,483,578 - -0.5 2,483,623 - +0.1 2,483,623 - -0.9 2,483,623 - -2.2 2,483,623 - -0.0 2,483,629 + -1.3 2,483,629 + +0.8 2,483,630 - -2.3 2,483,630 - -0.6 2,483,668 + +1.4 2,483,705 + Pf1N1B4_2170 0.12 -1.0 2,483,706 - Pf1N1B4_2170 0.12 -0.1 2,483,788 + Pf1N1B4_2170 0.24 +0.4 2,483,789 - Pf1N1B4_2170 0.24 -0.2 2,483,789 - Pf1N1B4_2170 0.24 -0.2 2,483,789 - Pf1N1B4_2170 0.24 +0.0 2,483,789 - Pf1N1B4_2170 0.24 +1.4 2,483,899 - Pf1N1B4_2170 0.40 +1.1 2,483,951 + Pf1N1B4_2170 0.47 +0.1 2,483,952 - Pf1N1B4_2170 0.47 +0.2 2,483,952 - Pf1N1B4_2170 0.47 +0.7 2,483,958 + Pf1N1B4_2170 0.48 +1.1 2,483,958 + Pf1N1B4_2170 0.48 +0.4 2,484,070 + Pf1N1B4_2170 0.64 -0.2 2,484,070 + Pf1N1B4_2170 0.64 -1.0 2,484,071 - Pf1N1B4_2170 0.64 -1.4 2,484,071 - Pf1N1B4_2170 0.64 +0.5 2,484,071 - Pf1N1B4_2170 0.64 +1.6 2,484,071 - Pf1N1B4_2170 0.64 -0.7 2,484,071 - Pf1N1B4_2170 0.64 +0.4 2,484,336 + +0.5 2,484,368 + +0.1 2,484,368 + -0.6 2,484,368 + -0.7 2,484,404 + +0.6 2,484,404 + -0.8 2,484,404 + -0.2 2,484,404 + -0.6 2,484,404 + +2.7 2,484,405 - -1.0 2,484,405 - +0.6 2,484,405 - -1.4 2,484,797 + Pf1N1B4_2171 0.53 -1.6 2,484,798 - Pf1N1B4_2171 0.53 +1.4 2,484,798 - Pf1N1B4_2171 0.53 -1.2 2,484,967 + Pf1N1B4_2171 0.72 +0.6 2,484,967 + Pf1N1B4_2171 0.72 -0.6 2,484,967 + Pf1N1B4_2171 0.72 -1.2 2,484,967 + Pf1N1B4_2171 0.72 -1.0 2,484,977 + Pf1N1B4_2171 0.73 -2.6 2,484,977 + Pf1N1B4_2171 0.73 +1.4 2,484,978 - Pf1N1B4_2171 0.73 +0.0 2,485,148 + +0.4 2,485,227 + -2.2 2,485,286 - +0.1 2,485,286 - -0.8 2,485,286 - +0.8 2,485,286 - +0.1 2,485,286 - -0.2 2,485,318 + Pf1N1B4_2172 0.13 -0.4 2,485,318 + Pf1N1B4_2172 0.13 -2.0 2,485,318 + Pf1N1B4_2172 0.13 +0.8 2,485,318 + Pf1N1B4_2172 0.13 -1.0 2,485,319 - +2.4 2,485,319 - Pf1N1B4_2172 0.13 -1.1 2,485,319 - Pf1N1B4_2172 0.13 -0.9 2,485,319 - Pf1N1B4_2172 0.13 +0.9 2,485,626 + Pf1N1B4_2172 0.56 +0.6 2,485,626 + Pf1N1B4_2172 0.56 -1.6 2,485,627 - Pf1N1B4_2172 0.56 +0.2 2,485,627 - Pf1N1B4_2172 0.56 -0.0 2,485,627 - Pf1N1B4_2172 0.56 +0.2 2,485,821 + Pf1N1B4_2172 0.83 -1.6 2,485,821 + Pf1N1B4_2172 0.83 +0.1 2,485,821 + Pf1N1B4_2172 0.83 -1.8 2,485,822 - Pf1N1B4_2172 0.83 -1.5 2,485,822 - Pf1N1B4_2172 0.83 +0.8 2,485,822 - Pf1N1B4_2172 0.83 +0.4 2,485,918 - -1.1 2,485,982 + -1.8 2,485,982 + -1.2 2,485,982 + +1.0 2,485,982 + -1.4 2,485,983 - +0.4 2,485,983 - -1.8 2,485,983 - +2.9 2,486,071 + Pf1N1B4_2173 0.13 -1.2
Or see this region's nucleotide sequence